The Effect of Mesenchymal Stem Cell-Enriched Scaffolds on MMP-8 and TGF-β Levels of Vertebrae Postlaminoplasty in Rabbit Model
Ahmad Jabir Rahyussalim,1– 3 Roni Eka Sahputra,4 Yanwirasti,5 Menkher Manjas,4 Yudan Whulanza,6 Tri Kurniawati,2 Dina Aprilya,1 Muhammad Luqman Labib Zufar1 1Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta...
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2021
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| Acceso en línea: | https://doaj.org/article/e1e2f5c7577b405eb31732b65cab4a69 |
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| Sumario: | Ahmad Jabir Rahyussalim,1– 3 Roni Eka Sahputra,4 Yanwirasti,5 Menkher Manjas,4 Yudan Whulanza,6 Tri Kurniawati,2 Dina Aprilya,1 Muhammad Luqman Labib Zufar1 1Department of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; 2Stem Cell and Tissue Engineering Cluster, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 3Stem Cell Medical Technology Integrated Service Unit, Cipto Mangunkusumo General Hospital, Jakarta, Indonesia; 4Department of Surgery, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; 5Department of Anatomy, Faculty of Medicine, Universitas Andalas-RSUP M. Djamil, Padang, Indonesia; 6Department of Mechanical Engineering, Faculty of Engineering, Universitas Indonesia, Jakarta, IndonesiaCorrespondence: Muhammad Luqman Labib ZufarDepartment of Orthopedics and Traumatology Clinics, Faculty of Medicine, Universitas of Indonesia-Cipto Mangunkusumo General Hospital, Jakarta, 10430, IndonesiaTel +6281385287727Email muhammad.luqman41@ui.ac.idIntroduction: Some laminoplasty procedures still have restenosis because of bony-bridging failure of the laminar hinge. The present study aimed to determine the effect of mesenchymal stem cell (MSC)-enriched scaffolds on vertebral regeneration after laminoplasty on the basis of the number of osteoblasts, matrix metalloproteinase-8 (MMP-8), and transforming growth factor-beta (TGF-β) levels.Methods: Laminoplasty procedure using the Hirabayashi technique was conducted at the lumbar level in 32 rabbits that were divided into four and three groups of the control (C) and treatment groups, respectively, with different types of laminoplasty spacer (T1, autograft; T2, scaffold; and T3, scaffold with MSCs). Histopathological studies were conducted to calculate the number of osteoblasts and enzyme-linked immunosorbent assay tests to detect MMP-8 and TGF-β 4 weeks after the surgery.Results: The results showed a significant decrease in MMP-8 level in the T3 group compared with that in the control group (p < 0.05). A significant difference exists between the average number of newly formed osteoblasts in the control group compared with that in the T3 group (p < 0.05) with a higher mean blood TGF-β level of all experimental groups compared with that of the control group (p = 0.58).Conclusion: The significant decrease in MMP-8 levels, increase in TGF-β levels, and increased number of osteoblasts on MSC-seeded polylactic acid scaffolds could be useful to support the laminoplasty procedure to prevent restenosis because it was biocompatible and promoted the bone healing process.Keywords: laminoplasty, Hirabayashi technique, mesenchymal stem cells, scaffolds |
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