Ion-dependent slow protein release from in vivo disintegrating micro-granules

Through the controlled addition of divalent cations, polyhistidine-tagged proteins can be clustered in form of chemically pure and mechanically stable micron-scale particles. Under physiological conditions, these materials act as self-disintegrating protein depots for the progressive release of the...

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Autores principales:	Patricia Álamo, Eloi Parladé, Hèctor López-Laguna, Eric Voltà-Durán, Ugutz Unzueta, Esther Vazquez, Ramon Mangues, Antonio Villaverde
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2021
Materias:	protein materials microparticles protein depots self-disintegrating materials tumor targeting Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/e1e64558485743c29d1660000687a2b5
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id	oai:doaj.org-article:e1e64558485743c29d1660000687a2b5
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spelling	oai:doaj.org-article:e1e64558485743c29d1660000687a2b52021-11-11T14:23:41ZIon-dependent slow protein release from in vivo disintegrating micro-granules1071-75441521-046410.1080/10717544.2021.1998249https://doaj.org/article/e1e64558485743c29d1660000687a2b52021-01-01T00:00:00Zhttp://dx.doi.org/10.1080/10717544.2021.1998249https://doaj.org/toc/1071-7544https://doaj.org/toc/1521-0464Through the controlled addition of divalent cations, polyhistidine-tagged proteins can be clustered in form of chemically pure and mechanically stable micron-scale particles. Under physiological conditions, these materials act as self-disintegrating protein depots for the progressive release of the forming polypeptide, with potential applications in protein drug delivery, diagnosis, or theragnosis. Here we have explored the in vivo disintegration pattern of a set of such depots, upon subcutaneous administration in mice. These microparticles were fabricated with cationic forms of either Zn, Ca, Mg, or Mn, which abound in the mammalian body. By using a CXCR4-targeted fluorescent protein as a reporter building block we categorized those cations regarding their ability to persist in the administration site and to sustain a slow release of functional protein. Ca2+ and specially Zn2+ have been observed as particularly good promoters of time-prolonged protein leakage. The released polypeptides result is available for selective molecular interactions, such as specific fluorescent labeling of tumor tissues, in which the protein reaches nearly steady levels.Patricia ÁlamoEloi ParladéHèctor López-LagunaEric Voltà-DuránUgutz UnzuetaEsther VazquezRamon ManguesAntonio VillaverdeTaylor & Francis Grouparticleprotein materialsmicroparticlesprotein depotsself-disintegrating materialstumor targetingTherapeutics. PharmacologyRM1-950ENDrug Delivery, Vol 28, Iss 1, Pp 2383-2391 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	protein materials microparticles protein depots self-disintegrating materials tumor targeting Therapeutics. Pharmacology RM1-950
spellingShingle	protein materials microparticles protein depots self-disintegrating materials tumor targeting Therapeutics. Pharmacology RM1-950 Patricia Álamo Eloi Parladé Hèctor López-Laguna Eric Voltà-Durán Ugutz Unzueta Esther Vazquez Ramon Mangues Antonio Villaverde Ion-dependent slow protein release from in vivo disintegrating micro-granules
description	Through the controlled addition of divalent cations, polyhistidine-tagged proteins can be clustered in form of chemically pure and mechanically stable micron-scale particles. Under physiological conditions, these materials act as self-disintegrating protein depots for the progressive release of the forming polypeptide, with potential applications in protein drug delivery, diagnosis, or theragnosis. Here we have explored the in vivo disintegration pattern of a set of such depots, upon subcutaneous administration in mice. These microparticles were fabricated with cationic forms of either Zn, Ca, Mg, or Mn, which abound in the mammalian body. By using a CXCR4-targeted fluorescent protein as a reporter building block we categorized those cations regarding their ability to persist in the administration site and to sustain a slow release of functional protein. Ca2+ and specially Zn2+ have been observed as particularly good promoters of time-prolonged protein leakage. The released polypeptides result is available for selective molecular interactions, such as specific fluorescent labeling of tumor tissues, in which the protein reaches nearly steady levels.
format	article
author	Patricia Álamo Eloi Parladé Hèctor López-Laguna Eric Voltà-Durán Ugutz Unzueta Esther Vazquez Ramon Mangues Antonio Villaverde
author_facet	Patricia Álamo Eloi Parladé Hèctor López-Laguna Eric Voltà-Durán Ugutz Unzueta Esther Vazquez Ramon Mangues Antonio Villaverde
author_sort	Patricia Álamo
title	Ion-dependent slow protein release from in vivo disintegrating micro-granules
title_short	Ion-dependent slow protein release from in vivo disintegrating micro-granules
title_full	Ion-dependent slow protein release from in vivo disintegrating micro-granules
title_fullStr	Ion-dependent slow protein release from in vivo disintegrating micro-granules
title_full_unstemmed	Ion-dependent slow protein release from in vivo disintegrating micro-granules
title_sort	ion-dependent slow protein release from in vivo disintegrating micro-granules
publisher	Taylor & Francis Group
publishDate	2021
url	https://doaj.org/article/e1e64558485743c29d1660000687a2b5
work_keys_str_mv	AT patriciaalamo iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT eloiparlade iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT hectorlopezlaguna iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT ericvoltaduran iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT ugutzunzueta iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT esthervazquez iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT ramonmangues iondependentslowproteinreleasefrominvivodisintegratingmicrogranules AT antoniovillaverde iondependentslowproteinreleasefrominvivodisintegratingmicrogranules
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Ion-dependent slow protein release from in vivo disintegrating micro-granules

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