Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells

Abstract Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Go Itakura, Masahiro Ozaki, Narihito Nagoshi, Soya Kawabata, Yuichiro Nishiyama, Keiko Sugai, Tsuyoshi Iida, Rei Kashiwagi, Toshiki Ookubo, Kaori Yastake, Kohei Matsubayashi, Jun Kohyama, Akio Iwanami, Morio Matsumoto, Masaya Nakamura, Hideyuki Okano
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1f32ce635554257b3877f4bba6341ec
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e1f32ce635554257b3877f4bba6341ec
record_format	dspace
spelling	oai:doaj.org-article:e1f32ce635554257b3877f4bba6341ec2021-12-02T15:05:11ZLow immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells10.1038/s41598-017-13522-w2045-2322https://doaj.org/article/e1f32ce635554257b3877f4bba6341ec2017-10-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-13522-whttps://doaj.org/toc/2045-2322Abstract Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenitor cells (iPSC-NSPCs) both in vitro and in vivo. Flow cytometry revealed the low expression of immunological surface antigens, and these cells survived in all mice when transplanted syngeneically into subcutaneous tissue and the spinal cord. In contrast, an allogeneic transplantation into subcutaneous tissue was rejected in all mice, and allogeneic cells transplanted into intact and injured spinal cords survived for 3 months in approximately 20% of mice. In addition, cell survival was increased after co-treatment with an immunosuppressive agent. Thus, the immunogenicity and post-transplantation immunological dynamics of iPSC-NSPCs resemble those of fetus-NSPCs.Go ItakuraMasahiro OzakiNarihito NagoshiSoya KawabataYuichiro NishiyamaKeiko SugaiTsuyoshi IidaRei KashiwagiToshiki OokuboKaori YastakeKohei MatsubayashiJun KohyamaAkio IwanamiMorio MatsumotoMasaya NakamuraHideyuki OkanoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-13 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Go Itakura Masahiro Ozaki Narihito Nagoshi Soya Kawabata Yuichiro Nishiyama Keiko Sugai Tsuyoshi Iida Rei Kashiwagi Toshiki Ookubo Kaori Yastake Kohei Matsubayashi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
description	Abstract Resolving the immunogenicity of cells derived from induced pluripotent stem cells (iPSCs) remains an important challenge for cell transplant strategies that use banked allogeneic cells. Thus, we evaluated the immunogenicity of mouse fetal neural stem/progenitor cells (fetus-NSPCs) and iPSC-derived neural stem/progenitor cells (iPSC-NSPCs) both in vitro and in vivo. Flow cytometry revealed the low expression of immunological surface antigens, and these cells survived in all mice when transplanted syngeneically into subcutaneous tissue and the spinal cord. In contrast, an allogeneic transplantation into subcutaneous tissue was rejected in all mice, and allogeneic cells transplanted into intact and injured spinal cords survived for 3 months in approximately 20% of mice. In addition, cell survival was increased after co-treatment with an immunosuppressive agent. Thus, the immunogenicity and post-transplantation immunological dynamics of iPSC-NSPCs resemble those of fetus-NSPCs.
format	article
author	Go Itakura Masahiro Ozaki Narihito Nagoshi Soya Kawabata Yuichiro Nishiyama Keiko Sugai Tsuyoshi Iida Rei Kashiwagi Toshiki Ookubo Kaori Yastake Kohei Matsubayashi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano
author_facet	Go Itakura Masahiro Ozaki Narihito Nagoshi Soya Kawabata Yuichiro Nishiyama Keiko Sugai Tsuyoshi Iida Rei Kashiwagi Toshiki Ookubo Kaori Yastake Kohei Matsubayashi Jun Kohyama Akio Iwanami Morio Matsumoto Masaya Nakamura Hideyuki Okano
author_sort	Go Itakura
title	Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
title_short	Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
title_full	Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
title_fullStr	Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
title_full_unstemmed	Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
title_sort	low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e1f32ce635554257b3877f4bba6341ec
work_keys_str_mv	AT goitakura lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT masahiroozaki lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT narihitonagoshi lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT soyakawabata lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT yuichironishiyama lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT keikosugai lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT tsuyoshiiida lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT reikashiwagi lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT toshikiookubo lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT kaoriyastake lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT koheimatsubayashi lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT junkohyama lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT akioiwanami lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT moriomatsumoto lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT masayanakamura lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells AT hideyukiokano lowimmunogenicityofmouseinducedpluripotentstemcellderivedneuralstemprogenitorcells
_version_	1718388927204687872

Low immunogenicity of mouse induced pluripotent stem cell-derived neural stem/progenitor cells

Ejemplares similares