Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach

Abstract Galactomannan is a polymer of high economic importance and is extracted from the seed endosperm of clusterbean (C. tetragonoloba). In the present study, we worked to reveal the stage-specific galactomannan biosynthesis and its regulation in clusterbean. Combined electron microscopy and bioc...

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Autores principales:	Sandhya Sharma, Anshika Tyagi, Harsha Srivastava, G. Ramakrishna, Priya Sharma, Amitha Mithra Sevanthi, Amolkumar U. Solanke, Ramavtar Sharma, Nagendra Kumar Singh, Tilak Raj Sharma, Kishor Gaikwad
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1f84aa2fd9547838112e79673b8fb6c
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spelling	oai:doaj.org-article:e1f84aa2fd9547838112e79673b8fb6c2021-12-02T14:21:53ZExploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach10.1038/s41598-021-83507-32045-2322https://doaj.org/article/e1f84aa2fd9547838112e79673b8fb6c2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83507-3https://doaj.org/toc/2045-2322Abstract Galactomannan is a polymer of high economic importance and is extracted from the seed endosperm of clusterbean (C. tetragonoloba). In the present study, we worked to reveal the stage-specific galactomannan biosynthesis and its regulation in clusterbean. Combined electron microscopy and biochemical analysis revealed high protein and gum content in RGC-936, while high oil bodies and low gum content in M-83. A comparative transcriptome study was performed between RGC-936 (high gum) and M-83 (low gum) varieties at three developmental stages viz. 25, 39, and 50 days after flowering (DAF). Total 209,525, 375,595 and 255,401 unigenes were found at 25, 39 and 50 DAF respectively. Differentially expressed genes (DEGs) analysis indicated a total of 5147 shared unigenes between the two genotypes. Overall expression levels of transcripts at 39DAF were higher than 50DAF and 25DAF. Besides, 691 (RGC-936) and 188 (M-83) candidate unigenes that encode for enzymes involved in the biosynthesis of galactomannan were identified and analyzed, and 15 key enzyme genes were experimentally validated by quantitative Real-Time PCR. Transcription factor (TF) WRKY was observed to be co-expressed with key genes of galactomannan biosynthesis at 39DAF. We conclude that WRKY might be a potential biotechnological target (subject to functional validation) for developing high gum content varieties.Sandhya SharmaAnshika TyagiHarsha SrivastavaG. RamakrishnaPriya SharmaAmitha Mithra SevanthiAmolkumar U. SolankeRamavtar SharmaNagendra Kumar SinghTilak Raj SharmaKishor GaikwadNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-12 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sandhya Sharma Anshika Tyagi Harsha Srivastava G. Ramakrishna Priya Sharma Amitha Mithra Sevanthi Amolkumar U. Solanke Ramavtar Sharma Nagendra Kumar Singh Tilak Raj Sharma Kishor Gaikwad Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
description	Abstract Galactomannan is a polymer of high economic importance and is extracted from the seed endosperm of clusterbean (C. tetragonoloba). In the present study, we worked to reveal the stage-specific galactomannan biosynthesis and its regulation in clusterbean. Combined electron microscopy and biochemical analysis revealed high protein and gum content in RGC-936, while high oil bodies and low gum content in M-83. A comparative transcriptome study was performed between RGC-936 (high gum) and M-83 (low gum) varieties at three developmental stages viz. 25, 39, and 50 days after flowering (DAF). Total 209,525, 375,595 and 255,401 unigenes were found at 25, 39 and 50 DAF respectively. Differentially expressed genes (DEGs) analysis indicated a total of 5147 shared unigenes between the two genotypes. Overall expression levels of transcripts at 39DAF were higher than 50DAF and 25DAF. Besides, 691 (RGC-936) and 188 (M-83) candidate unigenes that encode for enzymes involved in the biosynthesis of galactomannan were identified and analyzed, and 15 key enzyme genes were experimentally validated by quantitative Real-Time PCR. Transcription factor (TF) WRKY was observed to be co-expressed with key genes of galactomannan biosynthesis at 39DAF. We conclude that WRKY might be a potential biotechnological target (subject to functional validation) for developing high gum content varieties.
format	article
author	Sandhya Sharma Anshika Tyagi Harsha Srivastava G. Ramakrishna Priya Sharma Amitha Mithra Sevanthi Amolkumar U. Solanke Ramavtar Sharma Nagendra Kumar Singh Tilak Raj Sharma Kishor Gaikwad
author_facet	Sandhya Sharma Anshika Tyagi Harsha Srivastava G. Ramakrishna Priya Sharma Amitha Mithra Sevanthi Amolkumar U. Solanke Ramavtar Sharma Nagendra Kumar Singh Tilak Raj Sharma Kishor Gaikwad
author_sort	Sandhya Sharma
title	Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
title_short	Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
title_full	Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
title_fullStr	Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
title_full_unstemmed	Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
title_sort	exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e1f84aa2fd9547838112e79673b8fb6c
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Exploring the edible gum (galactomannan) biosynthesis and its regulation during pod developmental stages in clusterbean using comparative transcriptomic approach

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