Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis

Summary: Maintaining proteostasis is important for animal development. How proteostasis influences spermatogenesis that generates male gametes, spermatozoa, is not clear. We show that testis-specific paralog of ribosomal large subunit protein RPL39, RPL39L, is required for mouse spermatogenesis. Del...

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Autores principales:	Qianxing Zou, Lele Yang, Ruona Shi, Yuling Qi, Xiaofei Zhang, Huayu Qi
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Molecular biology Male reproductive endocrinology Developmental biology Science Q
Acceso en línea:	https://doaj.org/article/e1fab069b52f4af7a59cda8412ae2618
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spelling	oai:doaj.org-article:e1fab069b52f4af7a59cda8412ae26182021-11-18T04:51:15ZProteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis2589-004210.1016/j.isci.2021.103396https://doaj.org/article/e1fab069b52f4af7a59cda8412ae26182021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2589004221013675https://doaj.org/toc/2589-0042Summary: Maintaining proteostasis is important for animal development. How proteostasis influences spermatogenesis that generates male gametes, spermatozoa, is not clear. We show that testis-specific paralog of ribosomal large subunit protein RPL39, RPL39L, is required for mouse spermatogenesis. Deletion of Rpl39l in mouse caused reduced proliferation of spermatogonial stem cells, malformed sperm mitochondria and flagella, leading to sub-fertility in males. Biochemical analyses revealed that lack of RPL39L deteriorated protein synthesis and protein quality control in spermatogenic cells, partly due to reduced biogenesis of ribosomal subunits and ribosome homeostasis. RPL39/RPL39L is likely assembled into ribosomes via H/ACA domain containing NOP10 complex early in ribosome biogenesis pathway. Furthermore, Rpl39l null mice exhibited compromised regenerative spermatogenesis after chemical insult and early degenerative spermatogenesis in aging mice. These data demonstrate that maintaining proteostasis is important for spermatogenesis, of which ribosome homeostasis maintained by ribosomal proteins coordinates translation machinery to the regulation of cellular growth.Qianxing ZouLele YangRuona ShiYuling QiXiaofei ZhangHuayu QiElsevierarticleMolecular biologyMale reproductive endocrinologyDevelopmental biologyScienceQENiScience, Vol 24, Iss 12, Pp 103396- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Molecular biology Male reproductive endocrinology Developmental biology Science Q
spellingShingle	Molecular biology Male reproductive endocrinology Developmental biology Science Q Qianxing Zou Lele Yang Ruona Shi Yuling Qi Xiaofei Zhang Huayu Qi Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
description	Summary: Maintaining proteostasis is important for animal development. How proteostasis influences spermatogenesis that generates male gametes, spermatozoa, is not clear. We show that testis-specific paralog of ribosomal large subunit protein RPL39, RPL39L, is required for mouse spermatogenesis. Deletion of Rpl39l in mouse caused reduced proliferation of spermatogonial stem cells, malformed sperm mitochondria and flagella, leading to sub-fertility in males. Biochemical analyses revealed that lack of RPL39L deteriorated protein synthesis and protein quality control in spermatogenic cells, partly due to reduced biogenesis of ribosomal subunits and ribosome homeostasis. RPL39/RPL39L is likely assembled into ribosomes via H/ACA domain containing NOP10 complex early in ribosome biogenesis pathway. Furthermore, Rpl39l null mice exhibited compromised regenerative spermatogenesis after chemical insult and early degenerative spermatogenesis in aging mice. These data demonstrate that maintaining proteostasis is important for spermatogenesis, of which ribosome homeostasis maintained by ribosomal proteins coordinates translation machinery to the regulation of cellular growth.
format	article
author	Qianxing Zou Lele Yang Ruona Shi Yuling Qi Xiaofei Zhang Huayu Qi
author_facet	Qianxing Zou Lele Yang Ruona Shi Yuling Qi Xiaofei Zhang Huayu Qi
author_sort	Qianxing Zou
title	Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
title_short	Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
title_full	Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
title_fullStr	Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
title_full_unstemmed	Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis
title_sort	proteostasis regulated by testis-specific ribosomal protein rpl39l maintains mouse spermatogenesis
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e1fab069b52f4af7a59cda8412ae2618
work_keys_str_mv	AT qianxingzou proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis AT leleyang proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis AT ruonashi proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis AT yulingqi proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis AT xiaofeizhang proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis AT huayuqi proteostasisregulatedbytestisspecificribosomalproteinrpl39lmaintainsmousespermatogenesis
_version_	1718425007553511424

Proteostasis regulated by testis-specific ribosomal protein RPL39L maintains mouse spermatogenesis

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