Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC

Abstract Patients receiving lipid emulsions are at increased risk of contracting catheter-related bloodstream infections (CRBSIs) in the clinic. More than 15% of CRBSIs are polymicrobial. The objective of this study was to explore the effects of lipid emulsions on the formation of Escherichia coli (...

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Autores principales:	Shanshan Li, Wanshi Duan, Yujie Lei, Zhonghui Wang, Chaojiang Fu, Liang He, Zhenghai Shen, Minjie Li, Ying Chen, Yunchao Huang
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e1fbcd07ad874239836b07433188e4b5
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spelling	oai:doaj.org-article:e1fbcd07ad874239836b07433188e4b52021-12-02T16:46:34ZEffects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC10.1038/s41598-021-96385-62045-2322https://doaj.org/article/e1fbcd07ad874239836b07433188e4b52021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-96385-6https://doaj.org/toc/2045-2322Abstract Patients receiving lipid emulsions are at increased risk of contracting catheter-related bloodstream infections (CRBSIs) in the clinic. More than 15% of CRBSIs are polymicrobial. The objective of this study was to explore the effects of lipid emulsions on the formation of Escherichia coli (E. coli)–Candida albicans (C. albicans) mixed-species biofilms (BFs) on polyvinyl chloride (PVC) surfaces and the underlying mechanism. Mixed-species BFs were produced by coculturing E. coli and C. albicans with PVC in various concentrations of lipid emulsions. Crystal violet staining and XTT assays were performed to test the mixed-species BF biomass and the viability of microbes in the BFs. The microstructures of the BFs were observed by an approach that combined confocal laser scanning microscopy, fluorescence in situ hybridization, and scanning electron microscopy. The study found that lipid emulsions could promote the formation of E. coli–C. albicans mixed-species BFs, especially with 10% lipid emulsions. The mechanism by which lipid emulsions promote mixed-species BF formation may involve significant upregulation of the expression of the flhDC, iha, HTA1, and HWP1 genes, which are associated with bacterial motility, adhesion, and BF formation. The results derived from this study necessitate strict aseptic precautions when handling lipid emulsions and avoiding the use of high concentrations of lipid emulsions for as long as possible.Shanshan LiWanshi DuanYujie LeiZhonghui WangChaojiang FuLiang HeZhenghai ShenMinjie LiYing ChenYunchao HuangNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-10 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Shanshan Li Wanshi Duan Yujie Lei Zhonghui Wang Chaojiang Fu Liang He Zhenghai Shen Minjie Li Ying Chen Yunchao Huang Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
description	Abstract Patients receiving lipid emulsions are at increased risk of contracting catheter-related bloodstream infections (CRBSIs) in the clinic. More than 15% of CRBSIs are polymicrobial. The objective of this study was to explore the effects of lipid emulsions on the formation of Escherichia coli (E. coli)–Candida albicans (C. albicans) mixed-species biofilms (BFs) on polyvinyl chloride (PVC) surfaces and the underlying mechanism. Mixed-species BFs were produced by coculturing E. coli and C. albicans with PVC in various concentrations of lipid emulsions. Crystal violet staining and XTT assays were performed to test the mixed-species BF biomass and the viability of microbes in the BFs. The microstructures of the BFs were observed by an approach that combined confocal laser scanning microscopy, fluorescence in situ hybridization, and scanning electron microscopy. The study found that lipid emulsions could promote the formation of E. coli–C. albicans mixed-species BFs, especially with 10% lipid emulsions. The mechanism by which lipid emulsions promote mixed-species BF formation may involve significant upregulation of the expression of the flhDC, iha, HTA1, and HWP1 genes, which are associated with bacterial motility, adhesion, and BF formation. The results derived from this study necessitate strict aseptic precautions when handling lipid emulsions and avoiding the use of high concentrations of lipid emulsions for as long as possible.
format	article
author	Shanshan Li Wanshi Duan Yujie Lei Zhonghui Wang Chaojiang Fu Liang He Zhenghai Shen Minjie Li Ying Chen Yunchao Huang
author_facet	Shanshan Li Wanshi Duan Yujie Lei Zhonghui Wang Chaojiang Fu Liang He Zhenghai Shen Minjie Li Ying Chen Yunchao Huang
author_sort	Shanshan Li
title	Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
title_short	Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
title_full	Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
title_fullStr	Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
title_full_unstemmed	Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC
title_sort	effects of lipid emulsions on the formation of escherichia coli–candida albicans mixed-species biofilms on pvc
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e1fbcd07ad874239836b07433188e4b5
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Effects of lipid emulsions on the formation of Escherichia coli–Candida albicans mixed-species biofilms on PVC

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