Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice

Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure (ARF). Geumgwe-sinkihwan (GSH) was recorded in a traditional Chines medical book named “Bangyakhappyeon” in 1884. GSH has been used for treatment for patients with diabetes and glomerulonephritis caused by deficienc...

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Autores principales:	Byung Hyuk Han, Hyeon Kyoung Lee, Se Hoon Jang, Ai Lin Tai, Youn Jae Jang, Jung Joo Yoon, Hye Yoom Kim, Ho Sub Lee, Yun Jung Lee, Dae Gill Kang
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	geumgwe-sinkihwan ischemia/reperfusion creatinine NGAL KIM-1 fibrosis Nutrition. Foods and food supply TX341-641
Acceso en línea:	https://doaj.org/article/e1fd5f0864964e5dbdd9de17daa137e3
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spelling	oai:doaj.org-article:e1fd5f0864964e5dbdd9de17daa137e32021-11-25T18:34:43ZEffect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice10.3390/nu131138592072-6643https://doaj.org/article/e1fd5f0864964e5dbdd9de17daa137e32021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6643/13/11/3859https://doaj.org/toc/2072-6643Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure (ARF). Geumgwe-sinkihwan (GSH) was recorded in a traditional Chines medical book named “Bangyakhappyeon” in 1884. GSH has been used for treatment for patients with diabetes and glomerulonephritis caused by deficiency of kidney yang and insufficiency of kidney gi. Here we investigate the effects of GSH in mice model of ischemic acute kidney injury. The mice groups are as follows; sham group: C57BL6 male mice, I/R group: C57BL6 male mice with I/R surgery, GSH low group: I/R + 100 mg/kg/day GSH, and GSH high group: I/R + 300 mg/kg/day GSH. Ischemia was induced by clamping both renal arteries and reperfusion. Mice were orally given GSH (100 and 300 mg/kg/day) during 3 days after surgery. Treatment with GSH significantly ameliorated creatinine clearance, creatinine, and blood urea nitrogen levels. Treatment with GSH reduced neutrophil gelatinase associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1), specific renal injury markers. GSH also reduced the periodic acid–Schiff and picro sirius red staining intensity in kidney of I/R group. Western blot and real-time RT-qPCR analysis demonstrated that GSH decreased protein and mRNA expression levels of the inflammatory cytokines in I/R-induced ARF mice. Moreover, GSH inhibited protein and mRNA expression of inflammasome-related protein including NLRP3 (NOD-like receptor pyrin domain-containing protein 3, cryoprin), ASC (Apoptosis-associated speck-like protein containing a CARD), and caspase-1. These findings provided evidence that GSH ameliorates renal injury including metabolic dysfunction and inflammation via the inhibition of NLRP3-dependent inflammasome in I/R-induced ARF mice.Byung Hyuk HanHyeon Kyoung LeeSe Hoon JangAi Lin TaiYoun Jae JangJung Joo YoonHye Yoom KimHo Sub LeeYun Jung LeeDae Gill KangMDPI AGarticlegeumgwe-sinkihwanischemia/reperfusioncreatinineNGALKIM-1fibrosisNutrition. Foods and food supplyTX341-641ENNutrients, Vol 13, Iss 3859, p 3859 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	geumgwe-sinkihwan ischemia/reperfusion creatinine NGAL KIM-1 fibrosis Nutrition. Foods and food supply TX341-641
spellingShingle	geumgwe-sinkihwan ischemia/reperfusion creatinine NGAL KIM-1 fibrosis Nutrition. Foods and food supply TX341-641 Byung Hyuk Han Hyeon Kyoung Lee Se Hoon Jang Ai Lin Tai Youn Jae Jang Jung Joo Yoon Hye Yoom Kim Ho Sub Lee Yun Jung Lee Dae Gill Kang Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
description	Renal ischemia-reperfusion (I/R) injury is an important cause of acute renal failure (ARF). Geumgwe-sinkihwan (GSH) was recorded in a traditional Chines medical book named “Bangyakhappyeon” in 1884. GSH has been used for treatment for patients with diabetes and glomerulonephritis caused by deficiency of kidney yang and insufficiency of kidney gi. Here we investigate the effects of GSH in mice model of ischemic acute kidney injury. The mice groups are as follows; sham group: C57BL6 male mice, I/R group: C57BL6 male mice with I/R surgery, GSH low group: I/R + 100 mg/kg/day GSH, and GSH high group: I/R + 300 mg/kg/day GSH. Ischemia was induced by clamping both renal arteries and reperfusion. Mice were orally given GSH (100 and 300 mg/kg/day) during 3 days after surgery. Treatment with GSH significantly ameliorated creatinine clearance, creatinine, and blood urea nitrogen levels. Treatment with GSH reduced neutrophil gelatinase associated lipocalin (NGAL) and kidney injury molecule-1 (KIM-1), specific renal injury markers. GSH also reduced the periodic acid–Schiff and picro sirius red staining intensity in kidney of I/R group. Western blot and real-time RT-qPCR analysis demonstrated that GSH decreased protein and mRNA expression levels of the inflammatory cytokines in I/R-induced ARF mice. Moreover, GSH inhibited protein and mRNA expression of inflammasome-related protein including NLRP3 (NOD-like receptor pyrin domain-containing protein 3, cryoprin), ASC (Apoptosis-associated speck-like protein containing a CARD), and caspase-1. These findings provided evidence that GSH ameliorates renal injury including metabolic dysfunction and inflammation via the inhibition of NLRP3-dependent inflammasome in I/R-induced ARF mice.
format	article
author	Byung Hyuk Han Hyeon Kyoung Lee Se Hoon Jang Ai Lin Tai Youn Jae Jang Jung Joo Yoon Hye Yoom Kim Ho Sub Lee Yun Jung Lee Dae Gill Kang
author_facet	Byung Hyuk Han Hyeon Kyoung Lee Se Hoon Jang Ai Lin Tai Youn Jae Jang Jung Joo Yoon Hye Yoom Kim Ho Sub Lee Yun Jung Lee Dae Gill Kang
author_sort	Byung Hyuk Han
title	Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
title_short	Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
title_full	Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
title_fullStr	Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
title_full_unstemmed	Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice
title_sort	effect of geumgwe-sinkihwan on renal dysfunction in ischemia/reperfusion-induced acute renal failure mice
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e1fd5f0864964e5dbdd9de17daa137e3
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Effect of Geumgwe-Sinkihwan on Renal Dysfunction in Ischemia/Reperfusion-Induced Acute Renal Failure Mice

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