Effects of an antibacterial membrane on osteoblast-like cells in vitro

Jun Ye1, Qianqian Yao1, Anchun Mo2, Jing Nie2, Wenwen Liu1, Cui Ye1, Xianji Chen11State Key Laboratory of Oral Diseases, 2Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People&#39;s Republic of ChinaAbstract: Infection around membranes is often fo...

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Autores principales:	Ye J, Yao Q, Mo A, Nie J, Liu W, Ye C, Chen X
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e1ffb54ae9a849db90615066bdfd00c4
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spelling	oai:doaj.org-article:e1ffb54ae9a849db90615066bdfd00c42021-12-02T02:27:40ZEffects of an antibacterial membrane on osteoblast-like cells in vitro1176-91141178-2013https://doaj.org/article/e1ffb54ae9a849db90615066bdfd00c42011-09-01T00:00:00Zhttp://www.dovepress.com/effects-of-an-antibacterial-membrane-on-osteoblast-like-cells-in-vitro-a8206https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Jun Ye1, Qianqian Yao1, Anchun Mo2, Jing Nie2, Wenwen Liu1, Cui Ye1, Xianji Chen11State Key Laboratory of Oral Diseases, 2Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People&#39;s Republic of ChinaAbstract: Infection around membranes is often found in guided bone regeneration (GBR). The excellent antibacterial properties of Ag-nHA-nTiO2/polyamide-66 (PA66) nanocomposite membranes have been demonstrated previously. The aim of this study was to observe the microstructure of an Ag-nHA-nTiO2/PA66 membrane and its effects on osteoblast-like cells in vitro. An Ag-nHA-nTiO2/PA66 membrane was used in the experimental group, and both nHA/PA66 and expanded poly tetrafluroethylene (e-PTFE) membranes were set as control. MG63 osteoblast-like cells were cultured on the three kinds of membrane and tissue culture polystyrene (TCP). The microstructure of the above membranes and the cells adhered on them were detected by scanning electronic microscope (SEM). Cell proliferation was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell viability with a cell viability analyzer, and alkaline phosphatase (ALP) activity and Ca2+ concentration of osteoblast-like cell matrix by enzyme-linked immunosorbent assay. SEM showed that both Ag-nHA-nTiO2/PA66 membranes and nHA/PA66 membranes were composed of porous obverse face and smooth opposite face. The e-PTFE membranes showed elliptic surface structure with many tiny lined cracks. The MG63 cells adhered and proliferated well on all three kinds of membranes. Though cell viability on Ag-nHA-nTiO2/PA66 membranes was significantly lower than that of the control groups (P&nbsp;&lt; 0.05), MTT values, ALP activity, and Ca2+ concentration did not differ significantly among the three kinds of membranes (P&nbsp;&gt; 0.05). From these findings, it can be concluded that Ag-nHA-nTiO2/PA66 membranes are as biocompatible as nHA/PA66 membranes and TCP, thus may be applied safely in GBR.Keywords: guided bone regeneration, hydroxyapatite, polyamide-66, silver, TiO2, MG63, osteoblast-like cells&nbsp;Ye JYao QMo ANie JLiu WYe CChen XDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 1853-1861 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Ye J Yao Q Mo A Nie J Liu W Ye C Chen X Effects of an antibacterial membrane on osteoblast-like cells in vitro
description	Jun Ye1, Qianqian Yao1, Anchun Mo2, Jing Nie2, Wenwen Liu1, Cui Ye1, Xianji Chen11State Key Laboratory of Oral Diseases, 2Department of Oral Implant, West China College of Stomatology, Sichuan University, Chengdu, People&#39;s Republic of ChinaAbstract: Infection around membranes is often found in guided bone regeneration (GBR). The excellent antibacterial properties of Ag-nHA-nTiO2/polyamide-66 (PA66) nanocomposite membranes have been demonstrated previously. The aim of this study was to observe the microstructure of an Ag-nHA-nTiO2/PA66 membrane and its effects on osteoblast-like cells in vitro. An Ag-nHA-nTiO2/PA66 membrane was used in the experimental group, and both nHA/PA66 and expanded poly tetrafluroethylene (e-PTFE) membranes were set as control. MG63 osteoblast-like cells were cultured on the three kinds of membrane and tissue culture polystyrene (TCP). The microstructure of the above membranes and the cells adhered on them were detected by scanning electronic microscope (SEM). Cell proliferation was determined by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell viability with a cell viability analyzer, and alkaline phosphatase (ALP) activity and Ca2+ concentration of osteoblast-like cell matrix by enzyme-linked immunosorbent assay. SEM showed that both Ag-nHA-nTiO2/PA66 membranes and nHA/PA66 membranes were composed of porous obverse face and smooth opposite face. The e-PTFE membranes showed elliptic surface structure with many tiny lined cracks. The MG63 cells adhered and proliferated well on all three kinds of membranes. Though cell viability on Ag-nHA-nTiO2/PA66 membranes was significantly lower than that of the control groups (P&nbsp;&lt; 0.05), MTT values, ALP activity, and Ca2+ concentration did not differ significantly among the three kinds of membranes (P&nbsp;&gt; 0.05). From these findings, it can be concluded that Ag-nHA-nTiO2/PA66 membranes are as biocompatible as nHA/PA66 membranes and TCP, thus may be applied safely in GBR.Keywords: guided bone regeneration, hydroxyapatite, polyamide-66, silver, TiO2, MG63, osteoblast-like cells&nbsp;
format	article
author	Ye J Yao Q Mo A Nie J Liu W Ye C Chen X
author_facet	Ye J Yao Q Mo A Nie J Liu W Ye C Chen X
author_sort	Ye J
title	Effects of an antibacterial membrane on osteoblast-like cells in vitro
title_short	Effects of an antibacterial membrane on osteoblast-like cells in vitro
title_full	Effects of an antibacterial membrane on osteoblast-like cells in vitro
title_fullStr	Effects of an antibacterial membrane on osteoblast-like cells in vitro
title_full_unstemmed	Effects of an antibacterial membrane on osteoblast-like cells in vitro
title_sort	effects of an antibacterial membrane on osteoblast-like cells in vitro
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/e1ffb54ae9a849db90615066bdfd00c4
work_keys_str_mv	AT yej effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT yaoq effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT moa effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT niej effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT liuw effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT yec effectsofanantibacterialmembraneonosteoblastlikecellsinvitro AT chenx effectsofanantibacterialmembraneonosteoblastlikecellsinvitro
_version_	1718402499610673152

Effects of an antibacterial membrane on osteoblast-like cells in vitro

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