Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel

Abstract Secondary lymphoid organs are critical for regulating acquired immune responses. The aim of this study was to characterize the impact of spaceflight on secondary lymphoid organs at the molecular level. We analysed the spleens and lymph nodes from mice flown aboard the International Space St...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kenta Horie, Hiroki Sasanuma, Takashi Kudo, Shin-ichiro Fujita, Maki Miyauchi, Takahisa Miyao, Takao Seki, Nobuko Akiyama, Yuki Takakura, Miki Shimbo, Hyojung Jeon, Masaki Shirakawa, Dai Shiba, Nobuaki Yoshida, Masafumi Muratani, Satoru Takahashi, Taishin Akiyama
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e20455f99c9a4ddcbb21f6fed3409fee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e20455f99c9a4ddcbb21f6fed3409fee
record_format	dspace
spelling	oai:doaj.org-article:e20455f99c9a4ddcbb21f6fed3409fee2021-12-02T15:08:58ZDown-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel10.1038/s41598-019-44067-92045-2322https://doaj.org/article/e20455f99c9a4ddcbb21f6fed3409fee2019-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-019-44067-9https://doaj.org/toc/2045-2322Abstract Secondary lymphoid organs are critical for regulating acquired immune responses. The aim of this study was to characterize the impact of spaceflight on secondary lymphoid organs at the molecular level. We analysed the spleens and lymph nodes from mice flown aboard the International Space Station (ISS) in orbit for 35 days, as part of a Japan Aerospace Exploration Agency mission. During flight, half of the mice were exposed to 1 g by centrifuging in the ISS, to provide information regarding the effect of microgravity and 1 g exposure during spaceflight. Whole-transcript cDNA sequencing (RNA-Seq) analysis of the spleen suggested that erythrocyte-related genes regulated by the transcription factor GATA1 were significantly down-regulated in ISS-flown vs. ground control mice. GATA1 and Tal1 (regulators of erythropoiesis) mRNA expression was consistently reduced by approximately half. These reductions were not completely alleviated by 1 g exposure in the ISS, suggesting that the combined effect of space environments aside from microgravity could down-regulate gene expression in the spleen. Additionally, plasma immunoglobulin concentrations were slightly altered in ISS-flown mice. Overall, our data suggest that spaceflight might disturb the homeostatic gene expression of the spleen through a combination of microgravity and other environmental changes.Kenta HorieHiroki SasanumaTakashi KudoShin-ichiro FujitaMaki MiyauchiTakahisa MiyaoTakao SekiNobuko AkiyamaYuki TakakuraMiki ShimboHyojung JeonMasaki ShirakawaDai ShibaNobuaki YoshidaMasafumi MurataniSatoru TakahashiTaishin AkiyamaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 9, Iss 1, Pp 1-11 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kenta Horie Hiroki Sasanuma Takashi Kudo Shin-ichiro Fujita Maki Miyauchi Takahisa Miyao Takao Seki Nobuko Akiyama Yuki Takakura Miki Shimbo Hyojung Jeon Masaki Shirakawa Dai Shiba Nobuaki Yoshida Masafumi Muratani Satoru Takahashi Taishin Akiyama Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
description	Abstract Secondary lymphoid organs are critical for regulating acquired immune responses. The aim of this study was to characterize the impact of spaceflight on secondary lymphoid organs at the molecular level. We analysed the spleens and lymph nodes from mice flown aboard the International Space Station (ISS) in orbit for 35 days, as part of a Japan Aerospace Exploration Agency mission. During flight, half of the mice were exposed to 1 g by centrifuging in the ISS, to provide information regarding the effect of microgravity and 1 g exposure during spaceflight. Whole-transcript cDNA sequencing (RNA-Seq) analysis of the spleen suggested that erythrocyte-related genes regulated by the transcription factor GATA1 were significantly down-regulated in ISS-flown vs. ground control mice. GATA1 and Tal1 (regulators of erythropoiesis) mRNA expression was consistently reduced by approximately half. These reductions were not completely alleviated by 1 g exposure in the ISS, suggesting that the combined effect of space environments aside from microgravity could down-regulate gene expression in the spleen. Additionally, plasma immunoglobulin concentrations were slightly altered in ISS-flown mice. Overall, our data suggest that spaceflight might disturb the homeostatic gene expression of the spleen through a combination of microgravity and other environmental changes.
format	article
author	Kenta Horie Hiroki Sasanuma Takashi Kudo Shin-ichiro Fujita Maki Miyauchi Takahisa Miyao Takao Seki Nobuko Akiyama Yuki Takakura Miki Shimbo Hyojung Jeon Masaki Shirakawa Dai Shiba Nobuaki Yoshida Masafumi Muratani Satoru Takahashi Taishin Akiyama
author_facet	Kenta Horie Hiroki Sasanuma Takashi Kudo Shin-ichiro Fujita Maki Miyauchi Takahisa Miyao Takao Seki Nobuko Akiyama Yuki Takakura Miki Shimbo Hyojung Jeon Masaki Shirakawa Dai Shiba Nobuaki Yoshida Masafumi Muratani Satoru Takahashi Taishin Akiyama
author_sort	Kenta Horie
title	Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
title_short	Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
title_full	Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
title_fullStr	Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
title_full_unstemmed	Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
title_sort	down-regulation of gata1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e20455f99c9a4ddcbb21f6fed3409fee
work_keys_str_mv	AT kentahorie downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT hirokisasanuma downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT takashikudo downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT shinichirofujita downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT makimiyauchi downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT takahisamiyao downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT takaoseki downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT nobukoakiyama downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT yukitakakura downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT mikishimbo downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT hyojungjeon downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT masakishirakawa downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT daishiba downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT nobuakiyoshida downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT masafumimuratani downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT satorutakahashi downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel AT taishinakiyama downregulationofgata1dependenterythrocyterelatedgenesinthespleensofmiceexposedtoaspacetravel
_version_	1718387971781033984

Down-regulation of GATA1-dependent erythrocyte-related genes in the spleens of mice exposed to a space travel

Ejemplares similares