Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations

Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases...

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Autores principales:	Ozkan Aydemir, Benedicta Mensah, Patrick W. Marsh, Benjamin Abuaku, James Leslie Myers-Hansen, Jeffrey A. Bailey, Anita Ghansah
Formato:	article
Lenguaje:	EN
Publicado:	PeerJ Inc. 2021
Materias:	Malaria Plasmodium falciparum Drug resistance Molecular inversion probes Molecular surveillance Medicine R
Acceso en línea:	https://doaj.org/article/e20a1fa2d4284d5fa75b4791c9fbc788
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spelling	oai:doaj.org-article:e20a1fa2d4284d5fa75b4791c9fbc7882021-11-11T15:05:07ZImmediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations10.7717/peerj.117942167-8359https://doaj.org/article/e20a1fa2d4284d5fa75b4791c9fbc7882021-11-01T00:00:00Zhttps://peerj.com/articles/11794.pdfhttps://peerj.com/articles/11794/https://doaj.org/toc/2167-8359Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.Ozkan AydemirBenedicta MensahPatrick W. MarshBenjamin AbuakuJames Leslie Myers-HansenJeffrey A. BaileyAnita GhansahPeerJ Inc.articleMalariaPlasmodium falciparumDrug resistanceMolecular inversion probesMolecular surveillanceMedicineRENPeerJ, Vol 9, p e11794 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Malaria Plasmodium falciparum Drug resistance Molecular inversion probes Molecular surveillance Medicine R
spellingShingle	Malaria Plasmodium falciparum Drug resistance Molecular inversion probes Molecular surveillance Medicine R Ozkan Aydemir Benedicta Mensah Patrick W. Marsh Benjamin Abuaku James Leslie Myers-Hansen Jeffrey A. Bailey Anita Ghansah Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
description	Antimalarial resistance surveillance in sub-Saharan Africa is often constrained by logistical and financial challenges limiting its breadth and frequency. At two sites in Ghana, we have piloted a streamlined sample pooling process created immediately by sequential addition of positive malaria cases at the time of diagnostic testing. This streamlined process involving a single tube minimized clinical and laboratory work and provided accurate frequencies of all known drug resistance mutations after high-throughput targeted sequencing using molecular inversion probes. Our study validates this method as a cost-efficient, accurate and highly-scalable approach for drug resistance mutation monitoring that can potentially be applied to other infectious diseases such as tuberculosis.
format	article
author	Ozkan Aydemir Benedicta Mensah Patrick W. Marsh Benjamin Abuaku James Leslie Myers-Hansen Jeffrey A. Bailey Anita Ghansah
author_facet	Ozkan Aydemir Benedicta Mensah Patrick W. Marsh Benjamin Abuaku James Leslie Myers-Hansen Jeffrey A. Bailey Anita Ghansah
author_sort	Ozkan Aydemir
title	Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
title_short	Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
title_full	Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
title_fullStr	Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
title_full_unstemmed	Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
title_sort	immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations
publisher	PeerJ Inc.
publishDate	2021
url	https://doaj.org/article/e20a1fa2d4284d5fa75b4791c9fbc788
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Immediate pools of malaria infections at diagnosis combined with targeted deep sequencing accurately quantifies frequency of drug resistance mutations

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