Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents

Abstract Reduced knee weight-bearing from prescription or sedentary lifestyles are associated with cartilage degradation; effects on the meniscus are unclear. Rodents exposed to spaceflight or hind limb unloading (HLU) represent unique opportunities to evaluate this question. This study evaluated ar...

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Autores principales:	Andy T. Kwok, Nequesha S. Mohamed, Johannes F. Plate, Raghunatha R. Yammani, Samuel Rosas, Ted A. Bateman, Eric Livingston, Joseph E. Moore, Bethany A. Kerr, Jingyun Lee, Cristina M. Furdui, Li Tan, Mary L. Bouxsein, Virginia L. Ferguson, Louis S. Stodieck, David C. Zawieja, Michael D. Delp, Xiao W. Mao, Jeffrey S. Willey
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e20ccc74580a4e1287b71ce54f1f7858
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spelling	oai:doaj.org-article:e20ccc74580a4e1287b71ce54f1f78582021-12-02T15:53:10ZSpaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents10.1038/s41598-021-90010-22045-2322https://doaj.org/article/e20ccc74580a4e1287b71ce54f1f78582021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90010-2https://doaj.org/toc/2045-2322Abstract Reduced knee weight-bearing from prescription or sedentary lifestyles are associated with cartilage degradation; effects on the meniscus are unclear. Rodents exposed to spaceflight or hind limb unloading (HLU) represent unique opportunities to evaluate this question. This study evaluated arthritic changes in the medial knee compartment that bears the highest loads across the knee after actual and simulated spaceflight, and recovery with subsequent full weight-bearing. Cartilage and meniscal degradation in mice were measured via microCT, histology, and proteomics and/or biochemically after: (1) ~ 35 days on the International Space Station (ISS); (2) 13-days aboard the Space Shuttle Atlantis; or (3) 30 days of HLU, followed by a 49-day weight-bearing readaptation with/without exercise. Cartilage degradation post-ISS and HLU occurred at similar spatial locations, the tibial-femoral cartilage-cartilage contact point, with meniscal volume decline. Cartilage and meniscal glycosaminoglycan content were decreased in unloaded mice, with elevated catabolic enzymes (e.g., matrix metalloproteinases), and elevated oxidative stress and catabolic molecular pathway responses in menisci. After the 13-day Shuttle flight, meniscal degradation was observed. During readaptation, recovery of cartilage volume and thickness occurred with exercise. Reduced weight-bearing from either spaceflight or HLU induced an arthritic phenotype in cartilage and menisci, and exercise promoted recovery.Andy T. KwokNequesha S. MohamedJohannes F. PlateRaghunatha R. YammaniSamuel RosasTed A. BatemanEric LivingstonJoseph E. MooreBethany A. KerrJingyun LeeCristina M. FurduiLi TanMary L. BouxseinVirginia L. FergusonLouis S. StodieckDavid C. ZawiejaMichael D. DelpXiao W. MaoJeffrey S. WilleyNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-17 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andy T. Kwok Nequesha S. Mohamed Johannes F. Plate Raghunatha R. Yammani Samuel Rosas Ted A. Bateman Eric Livingston Joseph E. Moore Bethany A. Kerr Jingyun Lee Cristina M. Furdui Li Tan Mary L. Bouxsein Virginia L. Ferguson Louis S. Stodieck David C. Zawieja Michael D. Delp Xiao W. Mao Jeffrey S. Willey Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
description	Abstract Reduced knee weight-bearing from prescription or sedentary lifestyles are associated with cartilage degradation; effects on the meniscus are unclear. Rodents exposed to spaceflight or hind limb unloading (HLU) represent unique opportunities to evaluate this question. This study evaluated arthritic changes in the medial knee compartment that bears the highest loads across the knee after actual and simulated spaceflight, and recovery with subsequent full weight-bearing. Cartilage and meniscal degradation in mice were measured via microCT, histology, and proteomics and/or biochemically after: (1) ~ 35 days on the International Space Station (ISS); (2) 13-days aboard the Space Shuttle Atlantis; or (3) 30 days of HLU, followed by a 49-day weight-bearing readaptation with/without exercise. Cartilage degradation post-ISS and HLU occurred at similar spatial locations, the tibial-femoral cartilage-cartilage contact point, with meniscal volume decline. Cartilage and meniscal glycosaminoglycan content were decreased in unloaded mice, with elevated catabolic enzymes (e.g., matrix metalloproteinases), and elevated oxidative stress and catabolic molecular pathway responses in menisci. After the 13-day Shuttle flight, meniscal degradation was observed. During readaptation, recovery of cartilage volume and thickness occurred with exercise. Reduced weight-bearing from either spaceflight or HLU induced an arthritic phenotype in cartilage and menisci, and exercise promoted recovery.
format	article
author	Andy T. Kwok Nequesha S. Mohamed Johannes F. Plate Raghunatha R. Yammani Samuel Rosas Ted A. Bateman Eric Livingston Joseph E. Moore Bethany A. Kerr Jingyun Lee Cristina M. Furdui Li Tan Mary L. Bouxsein Virginia L. Ferguson Louis S. Stodieck David C. Zawieja Michael D. Delp Xiao W. Mao Jeffrey S. Willey
author_facet	Andy T. Kwok Nequesha S. Mohamed Johannes F. Plate Raghunatha R. Yammani Samuel Rosas Ted A. Bateman Eric Livingston Joseph E. Moore Bethany A. Kerr Jingyun Lee Cristina M. Furdui Li Tan Mary L. Bouxsein Virginia L. Ferguson Louis S. Stodieck David C. Zawieja Michael D. Delp Xiao W. Mao Jeffrey S. Willey
author_sort	Andy T. Kwok
title	Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
title_short	Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
title_full	Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
title_fullStr	Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
title_full_unstemmed	Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
title_sort	spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e20ccc74580a4e1287b71ce54f1f7858
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Spaceflight and hind limb unloading induces an arthritic phenotype in knee articular cartilage and menisci of rodents

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