Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.

<h4>Background</h4>Caries and periodontitis are important human diseases associated with formation of multi-species biofilms. The involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. This study established a basic in vitro sing...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kerstin Standar, Bernd Kreikemeyer, Sylvio Redanz, Wanja L Münter, Michael Laue, Andreas Podbielski
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2106d63ba524ed9a155cb5ac184f084
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e2106d63ba524ed9a155cb5ac184f084
record_format	dspace
spelling	oai:doaj.org-article:e2106d63ba524ed9a155cb5ac184f0842021-11-18T07:03:44ZSetup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.1932-620310.1371/journal.pone.0013135https://doaj.org/article/e2106d63ba524ed9a155cb5ac184f0842010-10-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20957048/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>Caries and periodontitis are important human diseases associated with formation of multi-species biofilms. The involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. This study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. The setup allows a rapid screening for effects in the mutual species interaction. Furthermore, it is easy to handle, inexpensive, and reproducible.<h4>Methods</h4>Streptococcus mitis, S. salivarius and S. sanguinis, typical inhabitants of the healthy oral cavity, S. mutans as main carriogenic species, and Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra, S. intermedius and Aggregatibacter actinomycetemcomitans as periodontitis-associated bacteria, were investigated for their biofilm forming ability. Different liquid growth media were evaluated. Safranin-staining allowed monitoring of biofilm formation under the chosen conditions. Viable counts and microscopy permitted investigation of biofilm behavior in mixed-species and transwell setups.<h4>Findings</h4>S. mitis, F. nucleatum, P. gingivalis and P. micra failed to form biofilm structures. S. mutans, S. sanguinis, S. intermedius and S. salivarius established abundant biofilm masses in CDM/sucrose. A. actinomycetemcomitans formed patchy monolayers. For in depth analysis S. mitis, S. mutans and A. actinomycetemcomitans were chosen, because i) they are representatives of the physiological-, cariogenic and periodontitis-associated bacterial flora, respectively and ii) their difference in their biofilm forming ability. Microscopic analysis confirmed the results of safranin staining. Investigation of two species combinations of S. mitis with either S. mutans or A. actinomycetemcomitans revealed bacterial interactions influencing biofilm mass, biofilm structure and cell viability.<h4>Conclusions</h4>This setup shows safranin staining, microscopic analysis and viable counts together are crucial for basic examination and evaluation of biofilms. Our experiment generated meaningful results, exemplified by the noted S. mitis influence, and allows a fast decision about the most important bacterial interactions which should be investigated in depth.Kerstin StandarBernd KreikemeyerSylvio RedanzWanja L MünterMichael LaueAndreas PodbielskiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 10 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kerstin Standar Bernd Kreikemeyer Sylvio Redanz Wanja L Münter Michael Laue Andreas Podbielski Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
description	<h4>Background</h4>Caries and periodontitis are important human diseases associated with formation of multi-species biofilms. The involved bacteria are intensively studied to understand the molecular basis of the interactions in such biofilms. This study established a basic in vitro single and mixed-species culture model for oral bacteria combining three complimentary methods. The setup allows a rapid screening for effects in the mutual species interaction. Furthermore, it is easy to handle, inexpensive, and reproducible.<h4>Methods</h4>Streptococcus mitis, S. salivarius and S. sanguinis, typical inhabitants of the healthy oral cavity, S. mutans as main carriogenic species, and Porphyromonas gingivalis, Fusobacterium nucleatum, Parvimonas micra, S. intermedius and Aggregatibacter actinomycetemcomitans as periodontitis-associated bacteria, were investigated for their biofilm forming ability. Different liquid growth media were evaluated. Safranin-staining allowed monitoring of biofilm formation under the chosen conditions. Viable counts and microscopy permitted investigation of biofilm behavior in mixed-species and transwell setups.<h4>Findings</h4>S. mitis, F. nucleatum, P. gingivalis and P. micra failed to form biofilm structures. S. mutans, S. sanguinis, S. intermedius and S. salivarius established abundant biofilm masses in CDM/sucrose. A. actinomycetemcomitans formed patchy monolayers. For in depth analysis S. mitis, S. mutans and A. actinomycetemcomitans were chosen, because i) they are representatives of the physiological-, cariogenic and periodontitis-associated bacterial flora, respectively and ii) their difference in their biofilm forming ability. Microscopic analysis confirmed the results of safranin staining. Investigation of two species combinations of S. mitis with either S. mutans or A. actinomycetemcomitans revealed bacterial interactions influencing biofilm mass, biofilm structure and cell viability.<h4>Conclusions</h4>This setup shows safranin staining, microscopic analysis and viable counts together are crucial for basic examination and evaluation of biofilms. Our experiment generated meaningful results, exemplified by the noted S. mitis influence, and allows a fast decision about the most important bacterial interactions which should be investigated in depth.
format	article
author	Kerstin Standar Bernd Kreikemeyer Sylvio Redanz Wanja L Münter Michael Laue Andreas Podbielski
author_facet	Kerstin Standar Bernd Kreikemeyer Sylvio Redanz Wanja L Münter Michael Laue Andreas Podbielski
author_sort	Kerstin Standar
title	Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
title_short	Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
title_full	Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
title_fullStr	Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
title_full_unstemmed	Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
title_sort	setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e2106d63ba524ed9a155cb5ac184f084
work_keys_str_mv	AT kerstinstandar setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens AT berndkreikemeyer setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens AT sylvioredanz setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens AT wanjalmunter setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens AT michaellaue setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens AT andreaspodbielski setupofaninvitrotestsystemforbasicstudiesonbiofilmbehaviorofmixedspeciescultureswithdentalandperiodontalpathogens
_version_	1718424011132633088

Setup of an in vitro test system for basic studies on biofilm behavior of mixed-species cultures with dental and periodontal pathogens.

Ejemplares similares