Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice

Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism...

Descripción completa

Guardado en:
Detalles Bibliográficos
Autores principales: Fatemeh Ashrafian, Shahrbanoo Keshavarz Azizi Raftar, Arefeh Shahryari, Ava Behrouzi, Rezvan Yaghoubfar, Arezou Lari, Hamid Reza Moradi, Shohreh Khatami, Mir Davood Omrani, Farzam Vaziri, Andrea Masotti, Seyed Davar Siadat
Formato: article
Lenguaje:EN
Publicado: Nature Portfolio 2021
Materias:
R
Q
Acceso en línea:https://doaj.org/article/e21552090c09499a92bff91f6f05a073
Etiquetas: Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
id oai:doaj.org-article:e21552090c09499a92bff91f6f05a073
record_format dspace
spelling oai:doaj.org-article:e21552090c09499a92bff91f6f05a0732021-12-02T14:58:46ZComparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice10.1038/s41598-021-95738-52045-2322https://doaj.org/article/e21552090c09499a92bff91f6f05a0732021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95738-5https://doaj.org/toc/2045-2322Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism, and gut microbial composition in normal-diet fed mice as well as direct effects of the bacterium on Caco-2 cell line. A total of 30 mice were distributed into three different groups, control, alive, and pasteurized A. muciniphila-treated group. After acclimation, control and treatment groups were administrated with PBS and 109 CFU/200µL of bacterial suspension for 5 weeks, respectively. Besides, Caco-2 separately exposed to alive, pasteurized A. muciniphila and PBS for 24 h. The results showed that administration of A. muciniphila leads to reduction in body, liver, and white adipose weight. Histology data revealed both treatments had no adverse effects in colon, liver, and adipose tissues as well as induced better gut structure. Moreover, biochemical parameters and inflammatory biomarkers in plasma demonstrated that pasteurized A. muciniphila had more pronounce effect. Furthermore, alive A. muciniphia had better effects on the modulation of gene expression related to fatty acid synthesis, energy homeostasis, and immune response in the liver; meanwhile, these effects in the adipose was more in the pasteurized A. muciniphila administration. More importantly, the improvement of gut health by enhancing strengthen intestinal integrity and maintaining immune homeostasis was seen in both treatments; notably, pasteurized A. muciniphila had more effective. Similarly, treatment with the pasteurized form more effectively upregulated tight junction and regulated immune response-related genes in Caco-2 cell line. Both treatments triggered the improvement of microbiota communities, particularly the alive form. Therefore, both forms of A. muciniphila could modulate lipid and immune homeostasis, improved some gut microbiota, and promoted the overall health, while all these effects were dominantly observed in pasteurized form. In conclusion, pasteurized A. muciniphila can be considered as new medical supplement to maintain health state and prevent diseases in normal mice through different mechanisms.Fatemeh AshrafianShahrbanoo Keshavarz Azizi RaftarArefeh ShahryariAva BehrouziRezvan YaghoubfarArezou LariHamid Reza MoradiShohreh KhatamiMir Davood OmraniFarzam VaziriAndrea MasottiSeyed Davar SiadatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution DOAJ
collection DOAJ
language EN
topic Medicine
R
Science
Q
spellingShingle Medicine
R
Science
Q
Fatemeh Ashrafian
Shahrbanoo Keshavarz Azizi Raftar
Arefeh Shahryari
Ava Behrouzi
Rezvan Yaghoubfar
Arezou Lari
Hamid Reza Moradi
Shohreh Khatami
Mir Davood Omrani
Farzam Vaziri
Andrea Masotti
Seyed Davar Siadat
Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
description Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism, and gut microbial composition in normal-diet fed mice as well as direct effects of the bacterium on Caco-2 cell line. A total of 30 mice were distributed into three different groups, control, alive, and pasteurized A. muciniphila-treated group. After acclimation, control and treatment groups were administrated with PBS and 109 CFU/200µL of bacterial suspension for 5 weeks, respectively. Besides, Caco-2 separately exposed to alive, pasteurized A. muciniphila and PBS for 24 h. The results showed that administration of A. muciniphila leads to reduction in body, liver, and white adipose weight. Histology data revealed both treatments had no adverse effects in colon, liver, and adipose tissues as well as induced better gut structure. Moreover, biochemical parameters and inflammatory biomarkers in plasma demonstrated that pasteurized A. muciniphila had more pronounce effect. Furthermore, alive A. muciniphia had better effects on the modulation of gene expression related to fatty acid synthesis, energy homeostasis, and immune response in the liver; meanwhile, these effects in the adipose was more in the pasteurized A. muciniphila administration. More importantly, the improvement of gut health by enhancing strengthen intestinal integrity and maintaining immune homeostasis was seen in both treatments; notably, pasteurized A. muciniphila had more effective. Similarly, treatment with the pasteurized form more effectively upregulated tight junction and regulated immune response-related genes in Caco-2 cell line. Both treatments triggered the improvement of microbiota communities, particularly the alive form. Therefore, both forms of A. muciniphila could modulate lipid and immune homeostasis, improved some gut microbiota, and promoted the overall health, while all these effects were dominantly observed in pasteurized form. In conclusion, pasteurized A. muciniphila can be considered as new medical supplement to maintain health state and prevent diseases in normal mice through different mechanisms.
format article
author Fatemeh Ashrafian
Shahrbanoo Keshavarz Azizi Raftar
Arefeh Shahryari
Ava Behrouzi
Rezvan Yaghoubfar
Arezou Lari
Hamid Reza Moradi
Shohreh Khatami
Mir Davood Omrani
Farzam Vaziri
Andrea Masotti
Seyed Davar Siadat
author_facet Fatemeh Ashrafian
Shahrbanoo Keshavarz Azizi Raftar
Arefeh Shahryari
Ava Behrouzi
Rezvan Yaghoubfar
Arezou Lari
Hamid Reza Moradi
Shohreh Khatami
Mir Davood Omrani
Farzam Vaziri
Andrea Masotti
Seyed Davar Siadat
author_sort Fatemeh Ashrafian
title Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_short Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_full Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_fullStr Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_full_unstemmed Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_sort comparative effects of alive and pasteurized akkermansia muciniphila on normal diet-fed mice
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/e21552090c09499a92bff91f6f05a073
work_keys_str_mv AT fatemehashrafian comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT shahrbanookeshavarzaziziraftar comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT arefehshahryari comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT avabehrouzi comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT rezvanyaghoubfar comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT arezoulari comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT hamidrezamoradi comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT shohrehkhatami comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT mirdavoodomrani comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT farzamvaziri comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT andreamasotti comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
AT seyeddavarsiadat comparativeeffectsofaliveandpasteurizedakkermansiamuciniphilaonnormaldietfedmice
_version_ 1718389296231088128