Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice

Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism...

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Autores principales:	Fatemeh Ashrafian, Shahrbanoo Keshavarz Azizi Raftar, Arefeh Shahryari, Ava Behrouzi, Rezvan Yaghoubfar, Arezou Lari, Hamid Reza Moradi, Shohreh Khatami, Mir Davood Omrani, Farzam Vaziri, Andrea Masotti, Seyed Davar Siadat
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e21552090c09499a92bff91f6f05a073
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spelling	oai:doaj.org-article:e21552090c09499a92bff91f6f05a0732021-12-02T14:58:46ZComparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice10.1038/s41598-021-95738-52045-2322https://doaj.org/article/e21552090c09499a92bff91f6f05a0732021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95738-5https://doaj.org/toc/2045-2322Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism, and gut microbial composition in normal-diet fed mice as well as direct effects of the bacterium on Caco-2 cell line. A total of 30 mice were distributed into three different groups, control, alive, and pasteurized A. muciniphila-treated group. After acclimation, control and treatment groups were administrated with PBS and 109 CFU/200µL of bacterial suspension for 5 weeks, respectively. Besides, Caco-2 separately exposed to alive, pasteurized A. muciniphila and PBS for 24 h. The results showed that administration of A. muciniphila leads to reduction in body, liver, and white adipose weight. Histology data revealed both treatments had no adverse effects in colon, liver, and adipose tissues as well as induced better gut structure. Moreover, biochemical parameters and inflammatory biomarkers in plasma demonstrated that pasteurized A. muciniphila had more pronounce effect. Furthermore, alive A. muciniphia had better effects on the modulation of gene expression related to fatty acid synthesis, energy homeostasis, and immune response in the liver; meanwhile, these effects in the adipose was more in the pasteurized A. muciniphila administration. More importantly, the improvement of gut health by enhancing strengthen intestinal integrity and maintaining immune homeostasis was seen in both treatments; notably, pasteurized A. muciniphila had more effective. Similarly, treatment with the pasteurized form more effectively upregulated tight junction and regulated immune response-related genes in Caco-2 cell line. Both treatments triggered the improvement of microbiota communities, particularly the alive form. Therefore, both forms of A. muciniphila could modulate lipid and immune homeostasis, improved some gut microbiota, and promoted the overall health, while all these effects were dominantly observed in pasteurized form. In conclusion, pasteurized A. muciniphila can be considered as new medical supplement to maintain health state and prevent diseases in normal mice through different mechanisms.Fatemeh AshrafianShahrbanoo Keshavarz Azizi RaftarArefeh ShahryariAva BehrouziRezvan YaghoubfarArezou LariHamid Reza MoradiShohreh KhatamiMir Davood OmraniFarzam VaziriAndrea MasottiSeyed Davar SiadatNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arefeh Shahryari Ava Behrouzi Rezvan Yaghoubfar Arezou Lari Hamid Reza Moradi Shohreh Khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
description	Abstract Recently, Akkermansia muciniphila an anaerobic member of the gut microbiota, has been proposed as a next-generation probiotic. The aim of this study was evaluation of the effect of alive and pasteurized A. muciniphila on health status, intestinal integrity, immune response, lipid metabolism, and gut microbial composition in normal-diet fed mice as well as direct effects of the bacterium on Caco-2 cell line. A total of 30 mice were distributed into three different groups, control, alive, and pasteurized A. muciniphila-treated group. After acclimation, control and treatment groups were administrated with PBS and 109 CFU/200µL of bacterial suspension for 5 weeks, respectively. Besides, Caco-2 separately exposed to alive, pasteurized A. muciniphila and PBS for 24 h. The results showed that administration of A. muciniphila leads to reduction in body, liver, and white adipose weight. Histology data revealed both treatments had no adverse effects in colon, liver, and adipose tissues as well as induced better gut structure. Moreover, biochemical parameters and inflammatory biomarkers in plasma demonstrated that pasteurized A. muciniphila had more pronounce effect. Furthermore, alive A. muciniphia had better effects on the modulation of gene expression related to fatty acid synthesis, energy homeostasis, and immune response in the liver; meanwhile, these effects in the adipose was more in the pasteurized A. muciniphila administration. More importantly, the improvement of gut health by enhancing strengthen intestinal integrity and maintaining immune homeostasis was seen in both treatments; notably, pasteurized A. muciniphila had more effective. Similarly, treatment with the pasteurized form more effectively upregulated tight junction and regulated immune response-related genes in Caco-2 cell line. Both treatments triggered the improvement of microbiota communities, particularly the alive form. Therefore, both forms of A. muciniphila could modulate lipid and immune homeostasis, improved some gut microbiota, and promoted the overall health, while all these effects were dominantly observed in pasteurized form. In conclusion, pasteurized A. muciniphila can be considered as new medical supplement to maintain health state and prevent diseases in normal mice through different mechanisms.
format	article
author	Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arefeh Shahryari Ava Behrouzi Rezvan Yaghoubfar Arezou Lari Hamid Reza Moradi Shohreh Khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat
author_facet	Fatemeh Ashrafian Shahrbanoo Keshavarz Azizi Raftar Arefeh Shahryari Ava Behrouzi Rezvan Yaghoubfar Arezou Lari Hamid Reza Moradi Shohreh Khatami Mir Davood Omrani Farzam Vaziri Andrea Masotti Seyed Davar Siadat
author_sort	Fatemeh Ashrafian
title	Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_short	Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_full	Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_fullStr	Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_full_unstemmed	Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice
title_sort	comparative effects of alive and pasteurized akkermansia muciniphila on normal diet-fed mice
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e21552090c09499a92bff91f6f05a073
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Comparative effects of alive and pasteurized Akkermansia muciniphila on normal diet-fed mice

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