DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches

Abstract. Trisyani N, Rahayu DA. 2020. DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches. Biodiversitas 21: 478-484. Solen spp. are shells with various morphological characteristics with a wide distribution of tropical and subtropical beaches, including Indonesia. The...

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Autores principales:	ninis trisyani margono, DWI ANGGOROWATI RAHAYU
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2020
Materias:	phylogenetic, solen spp. haplotype. genetic distance, coi gene Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e2180d8eb5d44c67961a3193119ff394
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spelling	oai:doaj.org-article:e2180d8eb5d44c67961a3193119ff3942021-11-21T22:01:10ZDNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches1412-033X2085-472210.13057/biodiv/d210207https://doaj.org/article/e2180d8eb5d44c67961a3193119ff3942020-01-01T00:00:00Zhttps://smujo.id/biodiv/article/view/4964https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Trisyani N, Rahayu DA. 2020. DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches. Biodiversitas 21: 478-484. Solen spp. are shells with various morphological characteristics with a wide distribution of tropical and subtropical beaches, including Indonesia. The identification of Solen spp. is generally based on its morphological characteristics. This method is very problematic due to specimens share similarity in morphology and color. This study was using DNA barcode as a molecular identification tool. The bivalve COI sequence was amplified using PCR and molecular phylogenetic analysis using the Neighbor-Joining method. The amplified COI gene has a length of about 665 bp. The purpose of this study was to evaluate genetic variation and compare the phylogenetic Solen spp. in Indonesian waters. The composition of the nucleotide bases of Solen spp. the comparative species are A = 26.79%, C = 23.16%, G = 19.17% and T = 30.93%. The total nucleotide base A + T was 57.72%, while G + C was 42.33%. The results of phylogenetic analysis showed that Solen spp. Cirebon and Jambi are in one clade with Solen regularis with genetic distance 0.000 - 0.002. Solen spp. Surabaya, Bangkalan, Pamekasan, and Sumenep are in separate clades and are related to Solen grandis, Solen stricus and Solen lamarckii with genetic distance from 0.146 - 0.156. The diversity of nucleotide was 0.9780 and was divided into 12 haplotypes.ninis trisyani margonoDWI ANGGOROWATI RAHAYUMBI & UNS Soloarticlephylogenetic, solen spp. haplotype. genetic distance, coi geneBiology (General)QH301-705.5ENBiodiversitas, Vol 21, Iss 2 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	phylogenetic, solen spp. haplotype. genetic distance, coi gene Biology (General) QH301-705.5
spellingShingle	phylogenetic, solen spp. haplotype. genetic distance, coi gene Biology (General) QH301-705.5 ninis trisyani margono DWI ANGGOROWATI RAHAYU DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
description	Abstract. Trisyani N, Rahayu DA. 2020. DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches. Biodiversitas 21: 478-484. Solen spp. are shells with various morphological characteristics with a wide distribution of tropical and subtropical beaches, including Indonesia. The identification of Solen spp. is generally based on its morphological characteristics. This method is very problematic due to specimens share similarity in morphology and color. This study was using DNA barcode as a molecular identification tool. The bivalve COI sequence was amplified using PCR and molecular phylogenetic analysis using the Neighbor-Joining method. The amplified COI gene has a length of about 665 bp. The purpose of this study was to evaluate genetic variation and compare the phylogenetic Solen spp. in Indonesian waters. The composition of the nucleotide bases of Solen spp. the comparative species are A = 26.79%, C = 23.16%, G = 19.17% and T = 30.93%. The total nucleotide base A + T was 57.72%, while G + C was 42.33%. The results of phylogenetic analysis showed that Solen spp. Cirebon and Jambi are in one clade with Solen regularis with genetic distance 0.000 - 0.002. Solen spp. Surabaya, Bangkalan, Pamekasan, and Sumenep are in separate clades and are related to Solen grandis, Solen stricus and Solen lamarckii with genetic distance from 0.146 - 0.156. The diversity of nucleotide was 0.9780 and was divided into 12 haplotypes.
format	article
author	ninis trisyani margono DWI ANGGOROWATI RAHAYU
author_facet	ninis trisyani margono DWI ANGGOROWATI RAHAYU
author_sort	ninis trisyani margono
title	DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
title_short	DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
title_full	DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
title_fullStr	DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
title_full_unstemmed	DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches
title_sort	dna barcoding of razor clam solen spp. (solinidae, bivalva) in indonesian beaches
publisher	MBI & UNS Solo
publishDate	2020
url	https://doaj.org/article/e2180d8eb5d44c67961a3193119ff394
work_keys_str_mv	AT ninistrisyanimargono dnabarcodingofrazorclamsolensppsolinidaebivalvainindonesianbeaches AT dwianggorowatirahayu dnabarcodingofrazorclamsolensppsolinidaebivalvainindonesianbeaches
_version_	1718418614150758400

DNA barcoding of razor clam Solen spp. (Solinidae, Bivalva) in Indonesian beaches

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