Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity

Haixia Lin,1,2,* Emir Tas,1– 5,* Elisabet Børsheim,1,2,4,5 Kelly E Mercer1,2,4 1Arkansas Children’s Nutrition Center, Little Rock, AR, USA; 2Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA; 3Endocrinology and Diabetes, Arkans...

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Autores principales:	Lin H, Tas E, Børsheim E, Mercer KE
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2020
Materias:	serum mirna insulin resistance adolescent obesity Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/e2191637be27423f8a76f0fef4e0072d
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spelling	oai:doaj.org-article:e2191637be27423f8a76f0fef4e0072d2021-12-02T12:33:20ZCirculating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity1178-7007https://doaj.org/article/e2191637be27423f8a76f0fef4e0072d2020-12-01T00:00:00Zhttps://www.dovepress.com/circulating-mirna-signatures-associated-with-insulin-resistance-in-ado-peer-reviewed-article-DMSOhttps://doaj.org/toc/1178-7007Haixia Lin,1,2,* Emir Tas,1– 5,* Elisabet Børsheim,1,2,4,5 Kelly E Mercer1,2,4 1Arkansas Children’s Nutrition Center, Little Rock, AR, USA; 2Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA; 3Endocrinology and Diabetes, Arkansas Children’s Hospital, Little Rock, AR, USA; 4Center for Childhood Obesity Prevention, Little Rock, AR, USA; 5Arkansas Children’s Research Institute, Little Rock, AR, USA*These authors contributed equally to this workCorrespondence: Kelly E Mercer; Haixia LinArkansas Children’s Nutrition Center, 15 Children’s Way, Little Rock, AR 72202, USATel +1-501-364-2706Fax +1-501-364-3161Email Kelly.Mercer@fda.hhs.gov; hlin@uams.eduPurpose: MicroRNAs (miRNAs) are implicated in metabolic changes accompanying progression of obesity, insulin resistance (IR), and metabolic disorders in children. Identifying circulating miRNAs that uniquely associate with these disorders may be useful in early identification and prevention of obesity-related complications. We aimed to identify circulating miRNA signatures that distinguish adolescents with obesity and IR from those with obesity unaccompanied by IR.Methods: Adolescents (aged 10– 17 years) with obesity were recruited from a weight management clinic. Fasting serum samples were obtained from 33 participants. A total of 179 miRNAs were queried by a quantitative RT-PCR-based miRNA focus panel. Differentially expressed miRNAs were compared between groups using Student’s t-test or one-way ANOVA analysis, and the association between IR evaluated by homeostatic model assessment model (HOMA-IR > 4) and body mass index (BMI) status was assessed using Pearson’s correlation analysis.Results: We found an expression pattern consisting of 12 elevated miRNAs linked to IR in obese adolescents. miR-30d, -221, and -122 were significantly correlated with clinical and biochemical markers of obesity and IR, suggestive of IR in adolescents at risk.Conclusion: Specific signatures of circulating miRNAs reflected metabolic phenotypes and predicted the presence of IR in adolescents with obesity, suggesting that miRNA indicators may identify obesity-associated complications in childhood. Further studies will be needed to understand cause versus effect and the mechanisms by which IR status links to changes in blood miRNA profiles.Keywords: serum miRNA, insulin resistance, adolescent, obesityLin HTas EBørsheim EMercer KEDove Medical Pressarticleserum mirnainsulin resistanceadolescentobesitySpecialties of internal medicineRC581-951ENDiabetes, Metabolic Syndrome and Obesity: Targets and Therapy, Vol Volume 13, Pp 4929-4939 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	serum mirna insulin resistance adolescent obesity Specialties of internal medicine RC581-951
spellingShingle	serum mirna insulin resistance adolescent obesity Specialties of internal medicine RC581-951 Lin H Tas E Børsheim E Mercer KE Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
description	Haixia Lin,1,2,* Emir Tas,1– 5,* Elisabet Børsheim,1,2,4,5 Kelly E Mercer1,2,4 1Arkansas Children’s Nutrition Center, Little Rock, AR, USA; 2Department of Pediatrics, University of Arkansas for Medical Sciences, Little Rock, AR, USA; 3Endocrinology and Diabetes, Arkansas Children’s Hospital, Little Rock, AR, USA; 4Center for Childhood Obesity Prevention, Little Rock, AR, USA; 5Arkansas Children’s Research Institute, Little Rock, AR, USA*These authors contributed equally to this workCorrespondence: Kelly E Mercer; Haixia LinArkansas Children’s Nutrition Center, 15 Children’s Way, Little Rock, AR 72202, USATel +1-501-364-2706Fax +1-501-364-3161Email Kelly.Mercer@fda.hhs.gov; hlin@uams.eduPurpose: MicroRNAs (miRNAs) are implicated in metabolic changes accompanying progression of obesity, insulin resistance (IR), and metabolic disorders in children. Identifying circulating miRNAs that uniquely associate with these disorders may be useful in early identification and prevention of obesity-related complications. We aimed to identify circulating miRNA signatures that distinguish adolescents with obesity and IR from those with obesity unaccompanied by IR.Methods: Adolescents (aged 10– 17 years) with obesity were recruited from a weight management clinic. Fasting serum samples were obtained from 33 participants. A total of 179 miRNAs were queried by a quantitative RT-PCR-based miRNA focus panel. Differentially expressed miRNAs were compared between groups using Student’s t-test or one-way ANOVA analysis, and the association between IR evaluated by homeostatic model assessment model (HOMA-IR > 4) and body mass index (BMI) status was assessed using Pearson’s correlation analysis.Results: We found an expression pattern consisting of 12 elevated miRNAs linked to IR in obese adolescents. miR-30d, -221, and -122 were significantly correlated with clinical and biochemical markers of obesity and IR, suggestive of IR in adolescents at risk.Conclusion: Specific signatures of circulating miRNAs reflected metabolic phenotypes and predicted the presence of IR in adolescents with obesity, suggesting that miRNA indicators may identify obesity-associated complications in childhood. Further studies will be needed to understand cause versus effect and the mechanisms by which IR status links to changes in blood miRNA profiles.Keywords: serum miRNA, insulin resistance, adolescent, obesity
format	article
author	Lin H Tas E Børsheim E Mercer KE
author_facet	Lin H Tas E Børsheim E Mercer KE
author_sort	Lin H
title	Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
title_short	Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
title_full	Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
title_fullStr	Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
title_full_unstemmed	Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity
title_sort	circulating mirna signatures associated with insulin resistance in adolescents with obesity
publisher	Dove Medical Press
publishDate	2020
url	https://doaj.org/article/e2191637be27423f8a76f0fef4e0072d
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Circulating miRNA Signatures Associated with Insulin Resistance in Adolescents with Obesity

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