Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy

Introduction and objectives: Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver abnormalities including steatosis, steatohepatitis, fibrosis, and cirrhosis. Liver biopsy remains the gold standard method to determine the disease stage in NAFLD but is an invasive and risky proce...

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Autores principales:	Aline Xavier, Flavia Zacconi, Fabián Santana-Romo, Thomas R. Eykyn, Begoña Lavin, Alkystis Phinikaridou, René Botnar, Sergio Uribe, Juan Esteban Oyarzún, Daniel Cabrera, Marco Arrese, Marcelo E. Andia
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Non-alcoholic fatty liver disease (NAFLD) Steatohepatitis Mouse model Magnetic resonance spectroscopy Gas chromatography with mass spectrometry (GC–MS) Choline-deficient, l-amino acid-defined (CDAA) Specialties of internal medicine RC581-951
Acceso en línea:	https://doaj.org/article/e21f3dc96e3648c897783a2431c6c217
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spelling	oai:doaj.org-article:e21f3dc96e3648c897783a2431c6c2172021-11-18T04:46:05ZAssessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy1665-268110.1016/j.aohep.2021.100358https://doaj.org/article/e21f3dc96e3648c897783a2431c6c2172021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1665268121000570https://doaj.org/toc/1665-2681Introduction and objectives: Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver abnormalities including steatosis, steatohepatitis, fibrosis, and cirrhosis. Liver biopsy remains the gold standard method to determine the disease stage in NAFLD but is an invasive and risky procedure. Studies have previously reported that changes in intrahepatic fatty acids (FA) composition are related to the progression of NAFLD, mainly in its early stages. The aim of this study was to characterize the liver FA composition in mice fed a Choline-deficient L-amino-defined (CDAA) diet at different stages of NAFLD using magnetic resonance spectroscopy (MRS). Methods: We used in-vivo MRS to perform a longitudinal characterization of hepatic FA changes in NAFLD mice for 10 weeks. We validated our findings with ex-vivo MRS, gas chromatography-mass spectrometry and histology. Results: In-vivo and ex-vivo results showed that livers from CDAA-fed mice exhibit a significant increase in liver FA content as well as a change in FA composition compared with control mice. After 4 weeks of CDAA diet, a decrease in polyunsaturated and an increase in monounsaturated FA were observed. These changes were associated with the appearance of early stages of steatohepatitis, confirmed by histology (NAFLD Activity Score (NAS) = 4.5). After 10 weeks of CDAA-diet, the liver FA composition remained stable while the NAS increased further to 6 showing a combination of early and late stages of steatohepatitis. Conclusion: Our results suggest that monitoring lipid composition in addition to total water/fat with MRS may yield additional insights that can be translated for non-invasive stratification of high-risk NAFLD patients.Aline XavierFlavia ZacconiFabián Santana-RomoThomas R. EykynBegoña LavinAlkystis PhinikaridouRené BotnarSergio UribeJuan Esteban OyarzúnDaniel CabreraMarco ArreseMarcelo E. AndiaElsevierarticleNon-alcoholic fatty liver disease (NAFLD)SteatohepatitisMouse modelMagnetic resonance spectroscopyGas chromatography with mass spectrometry (GC–MS)Choline-deficient, l-amino acid-defined (CDAA)Specialties of internal medicineRC581-951ENAnnals of Hepatology, Vol 25, Iss , Pp 100358- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Non-alcoholic fatty liver disease (NAFLD) Steatohepatitis Mouse model Magnetic resonance spectroscopy Gas chromatography with mass spectrometry (GC–MS) Choline-deficient, l-amino acid-defined (CDAA) Specialties of internal medicine RC581-951
spellingShingle	Non-alcoholic fatty liver disease (NAFLD) Steatohepatitis Mouse model Magnetic resonance spectroscopy Gas chromatography with mass spectrometry (GC–MS) Choline-deficient, l-amino acid-defined (CDAA) Specialties of internal medicine RC581-951 Aline Xavier Flavia Zacconi Fabián Santana-Romo Thomas R. Eykyn Begoña Lavin Alkystis Phinikaridou René Botnar Sergio Uribe Juan Esteban Oyarzún Daniel Cabrera Marco Arrese Marcelo E. Andia Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
description	Introduction and objectives: Non-alcoholic fatty liver disease (NAFLD) encompasses a spectrum of liver abnormalities including steatosis, steatohepatitis, fibrosis, and cirrhosis. Liver biopsy remains the gold standard method to determine the disease stage in NAFLD but is an invasive and risky procedure. Studies have previously reported that changes in intrahepatic fatty acids (FA) composition are related to the progression of NAFLD, mainly in its early stages. The aim of this study was to characterize the liver FA composition in mice fed a Choline-deficient L-amino-defined (CDAA) diet at different stages of NAFLD using magnetic resonance spectroscopy (MRS). Methods: We used in-vivo MRS to perform a longitudinal characterization of hepatic FA changes in NAFLD mice for 10 weeks. We validated our findings with ex-vivo MRS, gas chromatography-mass spectrometry and histology. Results: In-vivo and ex-vivo results showed that livers from CDAA-fed mice exhibit a significant increase in liver FA content as well as a change in FA composition compared with control mice. After 4 weeks of CDAA diet, a decrease in polyunsaturated and an increase in monounsaturated FA were observed. These changes were associated with the appearance of early stages of steatohepatitis, confirmed by histology (NAFLD Activity Score (NAS) = 4.5). After 10 weeks of CDAA-diet, the liver FA composition remained stable while the NAS increased further to 6 showing a combination of early and late stages of steatohepatitis. Conclusion: Our results suggest that monitoring lipid composition in addition to total water/fat with MRS may yield additional insights that can be translated for non-invasive stratification of high-risk NAFLD patients.
format	article
author	Aline Xavier Flavia Zacconi Fabián Santana-Romo Thomas R. Eykyn Begoña Lavin Alkystis Phinikaridou René Botnar Sergio Uribe Juan Esteban Oyarzún Daniel Cabrera Marco Arrese Marcelo E. Andia
author_facet	Aline Xavier Flavia Zacconi Fabián Santana-Romo Thomas R. Eykyn Begoña Lavin Alkystis Phinikaridou René Botnar Sergio Uribe Juan Esteban Oyarzún Daniel Cabrera Marco Arrese Marcelo E. Andia
author_sort	Aline Xavier
title	Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
title_short	Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
title_full	Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
title_fullStr	Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
title_full_unstemmed	Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
title_sort	assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e21f3dc96e3648c897783a2431c6c217
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Assessment of hepatic fatty acids during non-alcoholic steatohepatitis progression using magnetic resonance spectroscopy

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