DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.

Cell number changes during normal development, and in disease (e.g., neurodegeneration, cancer). Many genes affect cell number, thus functional genetic analysis frequently requires analysis of cell number alterations upon loss of function mutations or in gain of function experiments. Drosophila is a...

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Autores principales:	Manuel Guillermo Forero, Anabel R Learte, Stephanie Cartwright, Alicia Hidalgo
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2010
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2238bca10864305ac57e2458fa9d1fa
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spelling	oai:doaj.org-article:e2238bca10864305ac57e2458fa9d1fa2021-12-02T20:21:47ZDeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.1932-620310.1371/journal.pone.0010557https://doaj.org/article/e2238bca10864305ac57e2458fa9d1fa2010-05-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/20479944/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Cell number changes during normal development, and in disease (e.g., neurodegeneration, cancer). Many genes affect cell number, thus functional genetic analysis frequently requires analysis of cell number alterations upon loss of function mutations or in gain of function experiments. Drosophila is a most powerful model organism to investigate the function of genes involved in development or disease in vivo. Image processing and pattern recognition techniques can be used to extract information from microscopy images to quantify automatically distinct cellular features, but these methods are still not very extended in this model organism. Thus cellular quantification is often carried out manually, which is laborious, tedious, error prone or humanly unfeasible. Here, we present DeadEasy Mito-Glia, an image processing method to count automatically the number of mitotic cells labelled with anti-phospho-histone H3 and of glial cells labelled with anti-Repo in Drosophila embryos. This programme belongs to the DeadEasy suite of which we have previously developed versions to count apoptotic cells and neuronal nuclei. Having separate programmes is paramount for accuracy. DeadEasy Mito-Glia is very easy to use, fast, objective and very accurate when counting dividing cells and glial cells labelled with a nuclear marker. Although this method has been validated for Drosophila embryos, we provide an interactive window for biologists to easily extend its application to other nuclear markers and other sample types. DeadEasy MitoGlia is freely available as an ImageJ plug-in, it increases the repertoire of tools for in vivo genetic analysis, and it will be of interest to a broad community of developmental, cancer and neuro-biologists.Manuel Guillermo ForeroAnabel R LearteStephanie CartwrightAlicia HidalgoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 5, Iss 5, p e10557 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Manuel Guillermo Forero Anabel R Learte Stephanie Cartwright Alicia Hidalgo DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
description	Cell number changes during normal development, and in disease (e.g., neurodegeneration, cancer). Many genes affect cell number, thus functional genetic analysis frequently requires analysis of cell number alterations upon loss of function mutations or in gain of function experiments. Drosophila is a most powerful model organism to investigate the function of genes involved in development or disease in vivo. Image processing and pattern recognition techniques can be used to extract information from microscopy images to quantify automatically distinct cellular features, but these methods are still not very extended in this model organism. Thus cellular quantification is often carried out manually, which is laborious, tedious, error prone or humanly unfeasible. Here, we present DeadEasy Mito-Glia, an image processing method to count automatically the number of mitotic cells labelled with anti-phospho-histone H3 and of glial cells labelled with anti-Repo in Drosophila embryos. This programme belongs to the DeadEasy suite of which we have previously developed versions to count apoptotic cells and neuronal nuclei. Having separate programmes is paramount for accuracy. DeadEasy Mito-Glia is very easy to use, fast, objective and very accurate when counting dividing cells and glial cells labelled with a nuclear marker. Although this method has been validated for Drosophila embryos, we provide an interactive window for biologists to easily extend its application to other nuclear markers and other sample types. DeadEasy MitoGlia is freely available as an ImageJ plug-in, it increases the repertoire of tools for in vivo genetic analysis, and it will be of interest to a broad community of developmental, cancer and neuro-biologists.
format	article
author	Manuel Guillermo Forero Anabel R Learte Stephanie Cartwright Alicia Hidalgo
author_facet	Manuel Guillermo Forero Anabel R Learte Stephanie Cartwright Alicia Hidalgo
author_sort	Manuel Guillermo Forero
title	DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
title_short	DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
title_full	DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
title_fullStr	DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
title_full_unstemmed	DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.
title_sort	deadeasy mito-glia: automatic counting of mitotic cells and glial cells in drosophila.
publisher	Public Library of Science (PLoS)
publishDate	2010
url	https://doaj.org/article/e2238bca10864305ac57e2458fa9d1fa
work_keys_str_mv	AT manuelguillermoforero deadeasymitogliaautomaticcountingofmitoticcellsandglialcellsindrosophila AT anabelrlearte deadeasymitogliaautomaticcountingofmitoticcellsandglialcellsindrosophila AT stephaniecartwright deadeasymitogliaautomaticcountingofmitoticcellsandglialcellsindrosophila AT aliciahidalgo deadeasymitogliaautomaticcountingofmitoticcellsandglialcellsindrosophila
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DeadEasy Mito-Glia: automatic counting of mitotic cells and glial cells in Drosophila.

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