Cell mechanical properties of human breast carcinoma cells depend on temperature

Abstract The knowledge of cell mechanics is required to understand cellular processes and functions, such as the movement of cells, and the development of tissue engineering in cancer therapy. Cell mechanical properties depend on a variety of factors, such as cellular environments, and may also rely...

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Autores principales:	Christian Aermes, Alexander Hayn, Tony Fischer, Claudia Tanja Mierke
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e22d2900ebba416faa06c932736da35e
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spelling	oai:doaj.org-article:e22d2900ebba416faa06c932736da35e2021-12-02T15:00:13ZCell mechanical properties of human breast carcinoma cells depend on temperature10.1038/s41598-021-90173-y2045-2322https://doaj.org/article/e22d2900ebba416faa06c932736da35e2021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90173-yhttps://doaj.org/toc/2045-2322Abstract The knowledge of cell mechanics is required to understand cellular processes and functions, such as the movement of cells, and the development of tissue engineering in cancer therapy. Cell mechanical properties depend on a variety of factors, such as cellular environments, and may also rely on external factors, such as the ambient temperature. The impact of temperature on cell mechanics is not clearly understood. To explore the effect of temperature on cell mechanics, we employed magnetic tweezers to apply a force of 1 nN to 4.5 µm superparamagnetic beads. The beads were coated with fibronectin and coupled to human epithelial breast cancer cells, in particular MCF-7 and MDA-MB-231 cells. Cells were measured in a temperature range between 25 and 45 °C. The creep response of both cell types followed a weak power law. At all temperatures, the MDA-MB-231 cells were pronouncedly softer compared to the MCF-7 cells, whereas their fluidity was increased. However, with increasing temperature, the cells became significantly softer and more fluid. Since mechanical properties are manifested in the cell’s cytoskeletal structure and the paramagnetic beads are coupled through cell surface receptors linked to cytoskeletal structures, such as actin and myosin filaments as well as microtubules, the cells were probed with pharmacological drugs impacting the actin filament polymerization, such as Latrunculin A, the myosin filaments, such as Blebbistatin, and the microtubules, such as Demecolcine, during the magnetic tweezer measurements in the specific temperature range. Irrespective of pharmacological interventions, the creep response of cells followed a weak power law at all temperatures. Inhibition of the actin polymerization resulted in increased softness in both cell types and decreased fluidity exclusively in MDA-MB-231 cells. Blebbistatin had an effect on the compliance of MDA-MB-231 cells at lower temperatures, which was minor on the compliance MCF-7 cells. Microtubule inhibition affected the fluidity of MCF-7 cells but did not have a significant effect on the compliance of MCF-7 and MDA-MB-231 cells. In summary, with increasing temperature, the cells became significant softer with specific differences between the investigated drugs and cell lines.Christian AermesAlexander HaynTony FischerClaudia Tanja MierkeNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Christian Aermes Alexander Hayn Tony Fischer Claudia Tanja Mierke Cell mechanical properties of human breast carcinoma cells depend on temperature
description	Abstract The knowledge of cell mechanics is required to understand cellular processes and functions, such as the movement of cells, and the development of tissue engineering in cancer therapy. Cell mechanical properties depend on a variety of factors, such as cellular environments, and may also rely on external factors, such as the ambient temperature. The impact of temperature on cell mechanics is not clearly understood. To explore the effect of temperature on cell mechanics, we employed magnetic tweezers to apply a force of 1 nN to 4.5 µm superparamagnetic beads. The beads were coated with fibronectin and coupled to human epithelial breast cancer cells, in particular MCF-7 and MDA-MB-231 cells. Cells were measured in a temperature range between 25 and 45 °C. The creep response of both cell types followed a weak power law. At all temperatures, the MDA-MB-231 cells were pronouncedly softer compared to the MCF-7 cells, whereas their fluidity was increased. However, with increasing temperature, the cells became significantly softer and more fluid. Since mechanical properties are manifested in the cell’s cytoskeletal structure and the paramagnetic beads are coupled through cell surface receptors linked to cytoskeletal structures, such as actin and myosin filaments as well as microtubules, the cells were probed with pharmacological drugs impacting the actin filament polymerization, such as Latrunculin A, the myosin filaments, such as Blebbistatin, and the microtubules, such as Demecolcine, during the magnetic tweezer measurements in the specific temperature range. Irrespective of pharmacological interventions, the creep response of cells followed a weak power law at all temperatures. Inhibition of the actin polymerization resulted in increased softness in both cell types and decreased fluidity exclusively in MDA-MB-231 cells. Blebbistatin had an effect on the compliance of MDA-MB-231 cells at lower temperatures, which was minor on the compliance MCF-7 cells. Microtubule inhibition affected the fluidity of MCF-7 cells but did not have a significant effect on the compliance of MCF-7 and MDA-MB-231 cells. In summary, with increasing temperature, the cells became significant softer with specific differences between the investigated drugs and cell lines.
format	article
author	Christian Aermes Alexander Hayn Tony Fischer Claudia Tanja Mierke
author_facet	Christian Aermes Alexander Hayn Tony Fischer Claudia Tanja Mierke
author_sort	Christian Aermes
title	Cell mechanical properties of human breast carcinoma cells depend on temperature
title_short	Cell mechanical properties of human breast carcinoma cells depend on temperature
title_full	Cell mechanical properties of human breast carcinoma cells depend on temperature
title_fullStr	Cell mechanical properties of human breast carcinoma cells depend on temperature
title_full_unstemmed	Cell mechanical properties of human breast carcinoma cells depend on temperature
title_sort	cell mechanical properties of human breast carcinoma cells depend on temperature
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e22d2900ebba416faa06c932736da35e
work_keys_str_mv	AT christianaermes cellmechanicalpropertiesofhumanbreastcarcinomacellsdependontemperature AT alexanderhayn cellmechanicalpropertiesofhumanbreastcarcinomacellsdependontemperature AT tonyfischer cellmechanicalpropertiesofhumanbreastcarcinomacellsdependontemperature AT claudiatanjamierke cellmechanicalpropertiesofhumanbreastcarcinomacellsdependontemperature
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Cell mechanical properties of human breast carcinoma cells depend on temperature

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