RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53

Survival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulati...

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Autores principales:	Qing Tian, Chen Cao, Weijian Qiu, Han Wu, Lijun Zhou, Zhipeng Dai, Zhenwei Li, Songfeng Chen
Formato:	article
Lenguaje:	EN
Publicado:	Hindawi Limited 2021
Materias:	Internal medicine RC31-1245
Acceso en línea:	https://doaj.org/article/e235d15c963140de90940c29135daf1f
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spelling	oai:doaj.org-article:e235d15c963140de90940c29135daf1f2021-11-29T00:55:47ZRIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p531687-967810.1155/2021/5540149https://doaj.org/article/e235d15c963140de90940c29135daf1f2021-01-01T00:00:00Zhttp://dx.doi.org/10.1155/2021/5540149https://doaj.org/toc/1687-9678Survival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulation of RIPK1 on bone marrow mesenchymal stem cells (MSCs). Here, we have investigated for the first time the role of RIPK1 in bone marrow MSCs. We have found that RIPK1 knockdown suppressed proliferation, differentiation, and migration in bone marrow MSCs. Furthermore, RIPK1 knockdown resulted in the opening of mitochondrial permeability transition pore (mPTP) and mtDNA damage, leading to mitochondrial dysfunction, and consequently induced apoptosis and necroptosis in bone marrow MSCs. Moreover, we identified that the p53-PUMA axis pathway was involved in mitochondrial dysfunction in RIPK1-deficient bone marrow MSCs. Together, our findings highlighted that RIPK1 was indispensable for bone marrow MSC survival.Qing TianChen CaoWeijian QiuHan WuLijun ZhouZhipeng DaiZhenwei LiSongfeng ChenHindawi LimitedarticleInternal medicineRC31-1245ENStem Cells International, Vol 2021 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Internal medicine RC31-1245
spellingShingle	Internal medicine RC31-1245 Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
description	Survival of mesenchymal stem cells in the bone marrow is essential for bone marrow microenvironment homeostasis, but the molecular mechanisms remain poorly understood. RIPK1 has emerged as a critical molecule of programmed cell death in tissue homeostasis. However, little is known about the regulation of RIPK1 on bone marrow mesenchymal stem cells (MSCs). Here, we have investigated for the first time the role of RIPK1 in bone marrow MSCs. We have found that RIPK1 knockdown suppressed proliferation, differentiation, and migration in bone marrow MSCs. Furthermore, RIPK1 knockdown resulted in the opening of mitochondrial permeability transition pore (mPTP) and mtDNA damage, leading to mitochondrial dysfunction, and consequently induced apoptosis and necroptosis in bone marrow MSCs. Moreover, we identified that the p53-PUMA axis pathway was involved in mitochondrial dysfunction in RIPK1-deficient bone marrow MSCs. Together, our findings highlighted that RIPK1 was indispensable for bone marrow MSC survival.
format	article
author	Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen
author_facet	Qing Tian Chen Cao Weijian Qiu Han Wu Lijun Zhou Zhipeng Dai Zhenwei Li Songfeng Chen
author_sort	Qing Tian
title	RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
title_short	RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
title_full	RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
title_fullStr	RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
title_full_unstemmed	RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53
title_sort	ripk1 coordinates bone marrow mesenchymal stem cell survival by maintaining mitochondrial homeostasis via p53
publisher	Hindawi Limited
publishDate	2021
url	https://doaj.org/article/e235d15c963140de90940c29135daf1f
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_version_	1718407804651307008

RIPK1 Coordinates Bone Marrow Mesenchymal Stem Cell Survival by Maintaining Mitochondrial Homeostasis via p53

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