Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects...

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Autores principales:	Byul-Bora Choi, Jeong-Hae Choi, Tae-Hyung Kang, Seok-Jun Lee, Gyoo-Cheon Kim
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	differentiation no-ozone cold plasma osteoblast periodontal ligament cells Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e23bcb0eff1e43fa88fb56ad317568bc
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spelling	oai:doaj.org-article:e23bcb0eff1e43fa88fb56ad317568bc2021-11-25T16:48:48ZEnhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells10.3390/biomedicines91115422227-9059https://doaj.org/article/e23bcb0eff1e43fa88fb56ad317568bc2021-10-01T00:00:00Zhttps://www.mdpi.com/2227-9059/9/11/1542https://doaj.org/toc/2227-9059Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.Byul-Bora ChoiJeong-Hae ChoiTae-Hyung KangSeok-Jun LeeGyoo-Cheon KimMDPI AGarticledifferentiationno-ozone cold plasmaosteoblastperiodontal ligament cellsBiology (General)QH301-705.5ENBiomedicines, Vol 9, Iss 1542, p 1542 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	differentiation no-ozone cold plasma osteoblast periodontal ligament cells Biology (General) QH301-705.5
spellingShingle	differentiation no-ozone cold plasma osteoblast periodontal ligament cells Biology (General) QH301-705.5 Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
description	Periodontitis is an inflammatory disease that leads to periodontal tissue destruction and bone resorption. Proliferation and differentiation of cells capable of differentiating into osteoblasts is important for reconstructing periodontal tissues destroyed by periodontitis. In this study, the effects of the nozone (no-ozone) cold plasma (NCP) treatment on osteoblastic differentiation in periodontal ligament (PDL) cells were investigated. To test the toxicity of NCP on PDL cells, various NCP treatment methods and durations were tested, and time-dependent cell proliferation was analyzed using a water-soluble tetrazolium salts-1 assay. To determine the effect of NCP on PDL cell differentiation, the cells were provided with osteogenic media immediately after an NCP treatment to induce differentiation; the cells were then analyzed using alkaline phosphatase (ALP) staining, an ALP activity assay, real time PCR, and Alizarin Red S staining. The NCP treatment without toxicity on PDL cells was the condition of 1-min NCP treatment immediately followed by the replacement with fresh media. NCP increased ALP, osteocalcin, osteonectin, and osteopontin expression, as well as mineralization nodule formation. NCP treatment promotes osteoblastic differentiation of PDL cells; therefore, it may be beneficial for treating periodontitis.
format	article
author	Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim
author_facet	Byul-Bora Choi Jeong-Hae Choi Tae-Hyung Kang Seok-Jun Lee Gyoo-Cheon Kim
author_sort	Byul-Bora Choi
title	Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
title_short	Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
title_full	Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
title_fullStr	Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
title_full_unstemmed	Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells
title_sort	enhancement of osteoblast differentiation using no-ozone cold plasma on human periodontal ligament cells
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e23bcb0eff1e43fa88fb56ad317568bc
work_keys_str_mv	AT byulborachoi enhancementofosteoblastdifferentiationusingnoozonecoldplasmaonhumanperiodontalligamentcells AT jeonghaechoi enhancementofosteoblastdifferentiationusingnoozonecoldplasmaonhumanperiodontalligamentcells AT taehyungkang enhancementofosteoblastdifferentiationusingnoozonecoldplasmaonhumanperiodontalligamentcells AT seokjunlee enhancementofosteoblastdifferentiationusingnoozonecoldplasmaonhumanperiodontalligamentcells AT gyoocheonkim enhancementofosteoblastdifferentiationusingnoozonecoldplasmaonhumanperiodontalligamentcells
_version_	1718412989136109568

Enhancement of Osteoblast Differentiation Using No-Ozone Cold Plasma on Human Periodontal Ligament Cells

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