Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression

Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression

Zhiqing Lin, Lihua Liu, Zhuge Xi, Jiehua Huang, Bencheng LinInstitute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin, ChinaAbstract: The aim of this study was to ex...

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Autores principales: Lin ZQ, Liu LH, Xi ZG, Huang JH, Lin BC
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2012
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Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/e23d9290fd4742aca92673d47219e02b
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spelling oai:doaj.org-article:e23d9290fd4742aca92673d47219e02b2021-12-02T02:42:29ZSingle-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression1176-91141178-2013https://doaj.org/article/e23d9290fd4742aca92673d47219e02b2012-08-01T00:00:00Zhttp://www.dovepress.com/single-walled-carbon-nanotubes-promote-rat-vascular-adventitial-fibrob-a10582https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Zhiqing Lin, Lihua Liu, Zhuge Xi, Jiehua Huang, Bencheng LinInstitute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin, ChinaAbstract: The aim of this study was to explore whether single-wall carbon nanotubes (SWCNTs) can be used as artery tissue-engineering materials by promoting vascular adventitial fibroblasts (VAFs) to transform into myofibroblasts (MFs) and to find the signal pathway involved in this process. VAFs were primary cultured and incubated with various doses of SWCNTs suspension (0, 0.8, 3.2, 12.5, 50, and 200 µg/mL). In the present study, we used three methods (MTT, WST-1, and WST-8) at the same time to detect the cell viability and immunofluorescence probe technology to investigate the effects of oxidative injury after VAFs incubated with SWCNTs. Immunocytochemical staining was used to detect SM22-α expression to confirm whether VAFs transformed into MFs. The protein levels were detected by western blotting. The results of immunocytochemical staining showed that SM22-α was expressed after incubation with 50 µg/mL SWCNTs for 96 hours, but with oxidative damage. The mRNA and protein levels of SM22-α, C-Jun N-terminal kinase, TGF-β1, and TGF-β receptor II in VAFs increased with the dose of SWCNTs. The expression of the p-Smad2/3 protein was upregulated while the Smad7 protein was significantly down-regulated. Smad4 was translocated to the nucleus to regulate SM22-α gene expression. In conclusion, SWCNTs promoted VAFs to transform into MFs with SM22-α expression by the C-Jun N-terminal kinase/Smads signal pathway at the early stage (48 hours) but weakened quickly. SWCNTs also promoted the transformation by the TGF-β1/Smads signal pathway at the advanced stage in a persistent manner. These results indicate that SWCNTs can possibly be used as artery tissue-engineering materials.Keywords: SWCNTs, VAFs, SM22-α, signal pathway, tissue-engineering materialsLin ZQLiu LHXi ZGHuang JHLin BCDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2012, Iss default, Pp 4199-4206 (2012)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Lin ZQ
Liu LH
Xi ZG
Huang JH
Lin BC
Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
description Zhiqing Lin, Lihua Liu, Zhuge Xi, Jiehua Huang, Bencheng LinInstitute of Hygiene and Environmental Medicine, Academy of Military Medical Sciences, Tianjin Key Laboratory of Risk Assessment and Control Technology for Environment and Food Safety, Tianjin, ChinaAbstract: The aim of this study was to explore whether single-wall carbon nanotubes (SWCNTs) can be used as artery tissue-engineering materials by promoting vascular adventitial fibroblasts (VAFs) to transform into myofibroblasts (MFs) and to find the signal pathway involved in this process. VAFs were primary cultured and incubated with various doses of SWCNTs suspension (0, 0.8, 3.2, 12.5, 50, and 200 µg/mL). In the present study, we used three methods (MTT, WST-1, and WST-8) at the same time to detect the cell viability and immunofluorescence probe technology to investigate the effects of oxidative injury after VAFs incubated with SWCNTs. Immunocytochemical staining was used to detect SM22-α expression to confirm whether VAFs transformed into MFs. The protein levels were detected by western blotting. The results of immunocytochemical staining showed that SM22-α was expressed after incubation with 50 µg/mL SWCNTs for 96 hours, but with oxidative damage. The mRNA and protein levels of SM22-α, C-Jun N-terminal kinase, TGF-β1, and TGF-β receptor II in VAFs increased with the dose of SWCNTs. The expression of the p-Smad2/3 protein was upregulated while the Smad7 protein was significantly down-regulated. Smad4 was translocated to the nucleus to regulate SM22-α gene expression. In conclusion, SWCNTs promoted VAFs to transform into MFs with SM22-α expression by the C-Jun N-terminal kinase/Smads signal pathway at the early stage (48 hours) but weakened quickly. SWCNTs also promoted the transformation by the TGF-β1/Smads signal pathway at the advanced stage in a persistent manner. These results indicate that SWCNTs can possibly be used as artery tissue-engineering materials.Keywords: SWCNTs, VAFs, SM22-α, signal pathway, tissue-engineering materials
format article
author Lin ZQ
Liu LH
Xi ZG
Huang JH
Lin BC
author_facet Lin ZQ
Liu LH
Xi ZG
Huang JH
Lin BC
author_sort Lin ZQ
title Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
title_short Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
title_full Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
title_fullStr Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
title_full_unstemmed Single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by SM22-α expression
title_sort single-walled carbon nanotubes promote rat vascular adventitial fibroblasts to transform into myofibroblasts by sm22-α expression
publisher Dove Medical Press
publishDate 2012
url https://doaj.org/article/e23d9290fd4742aca92673d47219e02b
work_keys_str_mv AT linzq singlewalledcarbonnanotubespromoteratvascularadventitialfibroblaststotransformintomyofibroblastsbysm22ampalphaexpression
AT liulh singlewalledcarbonnanotubespromoteratvascularadventitialfibroblaststotransformintomyofibroblastsbysm22ampalphaexpression
AT xizg singlewalledcarbonnanotubespromoteratvascularadventitialfibroblaststotransformintomyofibroblastsbysm22ampalphaexpression
AT huangjh singlewalledcarbonnanotubespromoteratvascularadventitialfibroblaststotransformintomyofibroblastsbysm22ampalphaexpression
AT linbc singlewalledcarbonnanotubespromoteratvascularadventitialfibroblaststotransformintomyofibroblastsbysm22ampalphaexpression
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