<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages

ABSTRACT Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes the characterization of a yeast-specific CYP encoded by simA ("survival in macrophages"). Deletio...

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Autores principales:	Kylie J. Boyce, David P. De Souza, Saravanan Dayalan, Shivani Pasricha, Dedreia Tull, Malcolm J. McConville, Alex Andrianopoulos
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2018
Materias:	mycology dimorphism host-pathogen interactions Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e23d9ab14c8b4fe19edf0af8dfe52d7d
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spelling	oai:doaj.org-article:e23d9ab14c8b4fe19edf0af8dfe52d7d2021-11-15T15:22:14Z<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages10.1128/mSphere.00056-182379-5042https://doaj.org/article/e23d9ab14c8b4fe19edf0af8dfe52d7d2018-04-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mSphere.00056-18https://doaj.org/toc/2379-5042ABSTRACT Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes the characterization of a yeast-specific CYP encoded by simA ("survival in macrophages"). Deletion of simA does not affect yeast growth at 37°C in vitro but is essential for yeast cell production during macrophage infection. The ΔsimA strain exhibits reduced conidial germination and intracellular growth of yeast in macrophages, suggesting that the enzymatic product of SimA is required for normal fungal growth in vivo. Intracellular ΔsimA yeast cells exhibit cell wall defects, and metabolomic and chemical sensitivity data suggest that SimA may promote chitin synthesis or deposition in vitro. In vivo, ΔsimA yeast cells subsequently lyse and are degraded, suggesting that SimA may increase resistance to and/or suppress host cell biocidal effectors. The results suggest that simA synthesizes a secondary metabolite that allows T. marneffei to occupy the specific intracellular environmental niche within the macrophage. IMPORTANCE This study in a dimorphic fungal pathogen uncovered a role for a yeast-specific cytochrome P450 (CYP)-encoding gene in the ability of T. marneffei to grow as yeast cells within the host macrophages. This report will inspire further research into the role of CYPs and secondary metabolite synthesis during fungal pathogenic growth.Kylie J. BoyceDavid P. De SouzaSaravanan DayalanShivani PasrichaDedreia TullMalcolm J. McConvilleAlex AndrianopoulosAmerican Society for Microbiologyarticlemycologydimorphismhost-pathogen interactionsMicrobiologyQR1-502ENmSphere, Vol 3, Iss 2 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	mycology dimorphism host-pathogen interactions Microbiology QR1-502
spellingShingle	mycology dimorphism host-pathogen interactions Microbiology QR1-502 Kylie J. Boyce David P. De Souza Saravanan Dayalan Shivani Pasricha Dedreia Tull Malcolm J. McConville Alex Andrianopoulos <italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
description	ABSTRACT Fungi are adept at occupying specific environmental niches and often exploit numerous secondary metabolites generated by the cytochrome P450 (CYP) monoxygenases. This report describes the characterization of a yeast-specific CYP encoded by simA ("survival in macrophages"). Deletion of simA does not affect yeast growth at 37°C in vitro but is essential for yeast cell production during macrophage infection. The ΔsimA strain exhibits reduced conidial germination and intracellular growth of yeast in macrophages, suggesting that the enzymatic product of SimA is required for normal fungal growth in vivo. Intracellular ΔsimA yeast cells exhibit cell wall defects, and metabolomic and chemical sensitivity data suggest that SimA may promote chitin synthesis or deposition in vitro. In vivo, ΔsimA yeast cells subsequently lyse and are degraded, suggesting that SimA may increase resistance to and/or suppress host cell biocidal effectors. The results suggest that simA synthesizes a secondary metabolite that allows T. marneffei to occupy the specific intracellular environmental niche within the macrophage. IMPORTANCE This study in a dimorphic fungal pathogen uncovered a role for a yeast-specific cytochrome P450 (CYP)-encoding gene in the ability of T. marneffei to grow as yeast cells within the host macrophages. This report will inspire further research into the role of CYPs and secondary metabolite synthesis during fungal pathogenic growth.
format	article
author	Kylie J. Boyce David P. De Souza Saravanan Dayalan Shivani Pasricha Dedreia Tull Malcolm J. McConville Alex Andrianopoulos
author_facet	Kylie J. Boyce David P. De Souza Saravanan Dayalan Shivani Pasricha Dedreia Tull Malcolm J. McConville Alex Andrianopoulos
author_sort	Kylie J. Boyce
title	<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
title_short	<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
title_full	<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
title_fullStr	<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
title_full_unstemmed	<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages
title_sort	<italic toggle="yes">talaromyces marneffei sima</italic> encodes a fungal cytochrome p450 essential for survival in macrophages
publisher	American Society for Microbiology
publishDate	2018
url	https://doaj.org/article/e23d9ab14c8b4fe19edf0af8dfe52d7d
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<italic toggle="yes">Talaromyces marneffei simA</italic> Encodes a Fungal Cytochrome P450 Essential for Survival in Macrophages

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