Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans

Abstract Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane f...

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Autores principales:	Tao Zhang, Cheng Ni, Cheng Li, Pan Lu, Dan Chen, Yuanlin Dong, Johnathan R. Whetstine, Yiying Zhang, Zhongcong Xie
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e244fd4943fa4df084da1f7ef0b67eec
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spelling	oai:doaj.org-article:e244fd4943fa4df084da1f7ef0b67eec2021-12-02T16:14:10ZIsoflurane impairs oogenesis through germ cell apoptosis in C. elegans10.1038/s41598-021-93737-02045-2322https://doaj.org/article/e244fd4943fa4df084da1f7ef0b67eec2021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93737-0https://doaj.org/toc/2045-2322Abstract Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.Tao ZhangCheng NiCheng LiPan LuDan ChenYuanlin DongJohnathan R. WhetstineYiying ZhangZhongcong XieNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tao Zhang Cheng Ni Cheng Li Pan Lu Dan Chen Yuanlin Dong Johnathan R. Whetstine Yiying Zhang Zhongcong Xie Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
description	Abstract Anesthetic isoflurane has been reported to induce toxicity. However, the effects of isoflurane on fecundity remain largely unknown. We established a system in C. elegans to investigate the effects of isoflurane on oogenesis. Synchronized L4 stage C. elegans were treated with 7% isoflurane for 4 h. Dead cells, ROS, embryos, and unfertilized eggs laid by hermaphrodites were measured by fluorescence imaging and counting. The C. elegans with losses of ced-3, cep-1, abl-1, male C. elegans, and oxidative stress inhibitor N-acetyl-cysteine were used in the interaction studies. We found that isoflurane decreased the numbers of embryos and unfertilized eggs and increased the levels of dead cells and ROS in C. elegans. The isoflurane-induced impairment of oogenesis was associated with abl-1, ced-3, but not cep-1. N-acetyl-cysteine attenuated the isoflurane-induced impairment of oogenesis in C. elegans. Mating with male C. elegans did not attenuate the isoflurane-induced changes in oogenesis. These findings suggest that isoflurane may impair oogenesis through abl-1- and ced-3-associated, but not cep-1-associated, germ cell apoptosis and oxidative stress, pending further investigation. These studies will promote more research to determine the potential effects of anesthesia on fecundity.
format	article
author	Tao Zhang Cheng Ni Cheng Li Pan Lu Dan Chen Yuanlin Dong Johnathan R. Whetstine Yiying Zhang Zhongcong Xie
author_facet	Tao Zhang Cheng Ni Cheng Li Pan Lu Dan Chen Yuanlin Dong Johnathan R. Whetstine Yiying Zhang Zhongcong Xie
author_sort	Tao Zhang
title	Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
title_short	Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
title_full	Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
title_fullStr	Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
title_full_unstemmed	Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans
title_sort	isoflurane impairs oogenesis through germ cell apoptosis in c. elegans
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e244fd4943fa4df084da1f7ef0b67eec
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Isoflurane impairs oogenesis through germ cell apoptosis in C. elegans

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