Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

Zhen Yan,1,* Wenjun Wang,2,* Yongjun Wu,1,* Wei Wang,2 Bing Li,1 Ning Liang,1 Weidong Wu3 1College of Public Health, Zhengzhou University, Zhengzhou, 2School of Public Health, Jining Medical University, Jining, 3School of Public Health, Xinxiang Medical University, Xinxiang, People’s Repu...

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Autores principales:	Yan Z, Wang W, Wu Y, Li B, Liang N, Wu W
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Zinc oxide nanoparticles atherosclerosis lung inflammation heme oxygenase-1 platelet endothelial cell adhesion molecules-1 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e24aabb9b0f54f638adf98ab828dee70
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spelling	oai:doaj.org-article:e24aabb9b0f54f638adf98ab828dee702021-12-02T02:28:07ZZinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo1178-2013https://doaj.org/article/e24aabb9b0f54f638adf98ab828dee702017-06-01T00:00:00Zhttps://www.dovepress.com/zinc-oxide-nanoparticle-induced-atherosclerotic-alterations-in-vitro-a-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Zhen Yan,1,* Wenjun Wang,2,* Yongjun Wu,1,* Wei Wang,2 Bing Li,1 Ning Liang,1 Weidong Wu3 1College of Public Health, Zhengzhou University, Zhengzhou, 2School of Public Health, Jining Medical University, Jining, 3School of Public Health, Xinxiang Medical University, Xinxiang, People’s Republic of China *These authors contributed equally to this work Abstract: Engineered zinc oxide nanoparticles (ZnO-NPs) are currently being produced in high tonnage. Exposure to ZnO-NPs presents potential risks to cardiovascular system. Thus far, the toxicological effects of ZnO-NPs on cardiovascular system have not been well characterized. In this study, human coronary artery endothelial cells (HCAECs) were exposed to ZnO-NPs directly or indirectly using a transwell coculture system with human alveolar epithelial cell line A549 to mimic the lung/circulation interaction. It was shown that levels of proinflammatory mediators (interleukin-8 [IL-8] and tumor necrosis factor-α [TNF-α]) and biomarkers of atherosclerogenesis (heme oxygenase-1 [HO-1] and platelet endothelial cell adhesion molecules-1 [PECAM-1]) in the supernatants of culture media were significantly increased. Pretreatment of A549 cells on the apical side of the coculture system with the phagocytosis inhibitor cytochalasin B (CB) blocked ZnO-NP-induced HO-1 and PECAM-1 expression in HCAEC, indicating that endocytosis of ZnO-NPs by alveolar epithelial cells was involved in ZnO-NP-induced HO-1 or PECAM-1 expression in endothelial cells. Moreover, Wistar rats were intratracheally instilled with ZnO-NP suspension and high fat diet (positive control). ZnO-NP treatment induced lung and systemic inflammation, dyslipidemia, increased levels of serum HO-1 and PECAM-1, and aortic pathological damage. Taken together, exposure to ZnO-NPs could induce atherosclerotic alterations, which might involve phagocytosis of nanoparticles and inflammation in the lung. Keywords: zinc oxide nanoparticles, atherosclerosis, lung inflammation, heme oxygenase-1, platelet endothelial cell adhesion molecules-1 Yan ZWang WWu YWang WLi BLiang NWu WDove Medical PressarticleZinc oxide nanoparticlesatherosclerosislung inflammationheme oxygenase-1platelet endothelial cell adhesion molecules-1Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 12, Pp 4433-4442 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Zinc oxide nanoparticles atherosclerosis lung inflammation heme oxygenase-1 platelet endothelial cell adhesion molecules-1 Medicine (General) R5-920
spellingShingle	Zinc oxide nanoparticles atherosclerosis lung inflammation heme oxygenase-1 platelet endothelial cell adhesion molecules-1 Medicine (General) R5-920 Yan Z Wang W Wu Y Wang W Li B Liang N Wu W Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
description	Zhen Yan,1,* Wenjun Wang,2,* Yongjun Wu,1,* Wei Wang,2 Bing Li,1 Ning Liang,1 Weidong Wu3 1College of Public Health, Zhengzhou University, Zhengzhou, 2School of Public Health, Jining Medical University, Jining, 3School of Public Health, Xinxiang Medical University, Xinxiang, People’s Republic of China *These authors contributed equally to this work Abstract: Engineered zinc oxide nanoparticles (ZnO-NPs) are currently being produced in high tonnage. Exposure to ZnO-NPs presents potential risks to cardiovascular system. Thus far, the toxicological effects of ZnO-NPs on cardiovascular system have not been well characterized. In this study, human coronary artery endothelial cells (HCAECs) were exposed to ZnO-NPs directly or indirectly using a transwell coculture system with human alveolar epithelial cell line A549 to mimic the lung/circulation interaction. It was shown that levels of proinflammatory mediators (interleukin-8 [IL-8] and tumor necrosis factor-α [TNF-α]) and biomarkers of atherosclerogenesis (heme oxygenase-1 [HO-1] and platelet endothelial cell adhesion molecules-1 [PECAM-1]) in the supernatants of culture media were significantly increased. Pretreatment of A549 cells on the apical side of the coculture system with the phagocytosis inhibitor cytochalasin B (CB) blocked ZnO-NP-induced HO-1 and PECAM-1 expression in HCAEC, indicating that endocytosis of ZnO-NPs by alveolar epithelial cells was involved in ZnO-NP-induced HO-1 or PECAM-1 expression in endothelial cells. Moreover, Wistar rats were intratracheally instilled with ZnO-NP suspension and high fat diet (positive control). ZnO-NP treatment induced lung and systemic inflammation, dyslipidemia, increased levels of serum HO-1 and PECAM-1, and aortic pathological damage. Taken together, exposure to ZnO-NPs could induce atherosclerotic alterations, which might involve phagocytosis of nanoparticles and inflammation in the lung. Keywords: zinc oxide nanoparticles, atherosclerosis, lung inflammation, heme oxygenase-1, platelet endothelial cell adhesion molecules-1
format	article
author	Yan Z Wang W Wu Y Wang W Li B Liang N Wu W
author_facet	Yan Z Wang W Wu Y Wang W Li B Liang N Wu W
author_sort	Yan Z
title	Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
title_short	Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
title_full	Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
title_fullStr	Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
title_full_unstemmed	Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
title_sort	zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e24aabb9b0f54f638adf98ab828dee70
work_keys_str_mv	AT yanz zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT wangw zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT wuy zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT wangw zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT lib zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT liangn zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo AT wuw zincoxidenanoparticleinducedatheroscleroticalterationsinvitroandinvivo
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Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo

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