GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES

Graphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one w...

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Autores principales:	S. V. Uzhviyuk, M. S. Bochkova, V. P. Timganova, P. V. Khramtsov, K. Yu. Shardina, M. D. Kropaneva, A. I. Nechaev, M. B. Rayev, S. A. Zamorina
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2021
Materias:	graphene oxide surface modification of nanoparticles polyethyleneglycol luminol-dependent chemiluminescence monocytes reactive oxygen species Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/e2503c36b7d34cfc9f9d2ab7de92a4dc
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spelling	oai:doaj.org-article:e2503c36b7d34cfc9f9d2ab7de92a4dc2021-11-18T08:03:51ZGRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES1563-06252313-741X10.15789/1563-0625-GON-2207https://doaj.org/article/e2503c36b7d34cfc9f9d2ab7de92a4dc2021-10-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/2414https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XGraphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one way to reduce the harmful effects of graphene on cells. Applying nanoparticles implies their interaction with the immune system, which protects the body. Monocytes are innate immunity cells and the first line of defenсe of the human organism from microorganisms and other alien objects. One of the monocytes’ reactions to a stimulus of any nature is to produce reactive oxygen species (ROS). Published data shows an incomplete picture of modified graphene oxide nanoparticles’ effects on ROS formation by human monocytes. Thus, it was essential to evaluate the pegylated graphene oxide (GO-PEG and GO-8armedPEG) effect on ROS production by human monocytes, assessed by the luminol-dependent chemiluminescence (LCL). The objects of the study were CD14+-cells isolated from mononuclear cells of healthy donors. ROS production was stimulated by opsonized zymosan (OZ), spontaneous LCL was used as a control. PEG-modified (GO-PEG and GO-8armedPEG) GO nanoparticles with sizes of 100-200 nm (“small”) and 1-5 μm (“big”) with PEG covering ~ 20% were used at concentrations of 5 and 25 μg/ml. The study showed that small size nanoparticles at a low concentration of 5 μg/ml and big nanoparticles coated with 8-armed PEG at both concentrations have a significant suppressive effect on spontaneous ROS production. In the stimulated LCL reaction variant, it was found that small nanoparticles (25 μg/ml) also have a suppressive effect on ROS production, such as big-sized particles coated with linear PEG at the same concentration. Thus, we have established for the first time that graphene oxide nanoparticles functionalized with PEG are capable of inhibiting the ROS production by human monocytes, and therefore, we can speak of the antioxidant activity of GO-PEG.S. V. UzhviyukM. S. BochkovaV. P. TimganovaP. V. KhramtsovK. Yu. ShardinaM. D. KropanevaA. I. NechaevM. B. RayevS. A. ZamorinaSPb RAACIarticlegraphene oxidesurface modification of nanoparticlespolyethyleneglycolluminol-dependent chemiluminescencemonocytesreactive oxygen speciesImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 23, Iss 4, Pp 647-652 (2021)
institution	DOAJ
collection	DOAJ
language	RU
topic	graphene oxide surface modification of nanoparticles polyethyleneglycol luminol-dependent chemiluminescence monocytes reactive oxygen species Immunologic diseases. Allergy RC581-607
spellingShingle	graphene oxide surface modification of nanoparticles polyethyleneglycol luminol-dependent chemiluminescence monocytes reactive oxygen species Immunologic diseases. Allergy RC581-607 S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
description	Graphene-based materials have an opportunity for use in biomedicine, thanks to their properties. Nevertheless, due to its cytotoxic effects, the use of graphene-based drugs is problematic. However, the surface modification of graphene oxide (GO) nanoparticles with a polyethyleneglycol (PEG) is one way to reduce the harmful effects of graphene on cells. Applying nanoparticles implies their interaction with the immune system, which protects the body. Monocytes are innate immunity cells and the first line of defenсe of the human organism from microorganisms and other alien objects. One of the monocytes’ reactions to a stimulus of any nature is to produce reactive oxygen species (ROS). Published data shows an incomplete picture of modified graphene oxide nanoparticles’ effects on ROS formation by human monocytes. Thus, it was essential to evaluate the pegylated graphene oxide (GO-PEG and GO-8armedPEG) effect on ROS production by human monocytes, assessed by the luminol-dependent chemiluminescence (LCL). The objects of the study were CD14+-cells isolated from mononuclear cells of healthy donors. ROS production was stimulated by opsonized zymosan (OZ), spontaneous LCL was used as a control. PEG-modified (GO-PEG and GO-8armedPEG) GO nanoparticles with sizes of 100-200 nm (“small”) and 1-5 μm (“big”) with PEG covering ~ 20% were used at concentrations of 5 and 25 μg/ml. The study showed that small size nanoparticles at a low concentration of 5 μg/ml and big nanoparticles coated with 8-armed PEG at both concentrations have a significant suppressive effect on spontaneous ROS production. In the stimulated LCL reaction variant, it was found that small nanoparticles (25 μg/ml) also have a suppressive effect on ROS production, such as big-sized particles coated with linear PEG at the same concentration. Thus, we have established for the first time that graphene oxide nanoparticles functionalized with PEG are capable of inhibiting the ROS production by human monocytes, and therefore, we can speak of the antioxidant activity of GO-PEG.
format	article
author	S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina
author_facet	S. V. Uzhviyuk M. S. Bochkova V. P. Timganova P. V. Khramtsov K. Yu. Shardina M. D. Kropaneva A. I. Nechaev M. B. Rayev S. A. Zamorina
author_sort	S. V. Uzhviyuk
title	GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
title_short	GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
title_full	GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
title_fullStr	GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
title_full_unstemmed	GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES
title_sort	graphene oxide nanoparticles in the regulation of the oxidative activity of human monocytes
publisher	SPb RAACI
publishDate	2021
url	https://doaj.org/article/e2503c36b7d34cfc9f9d2ab7de92a4dc
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GRAPHENE OXIDE NANOPARTICLES IN THE REGULATION OF THE OXIDATIVE ACTIVITY OF HUMAN MONOCYTES

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