CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

Inducible genome editing systems often suffer from leakiness or reduced activity. Here the authors develop CRISPR-Switch, a Cre recombinase ON/OFF-controlled sgRNA cassette that allows consecutive editing of two loci.

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Autores principales:	Krzysztof Chylinski, Maria Hubmann, Ruth E. Hanna, Connor Yanchus, Georg Michlits, Esther C. H. Uijttewaal, John Doench, Daniel Schramek, Ulrich Elling
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2019
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e256e98fa6a7453b8ef036e4dd9081b9
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spelling	oai:doaj.org-article:e256e98fa6a7453b8ef036e4dd9081b92021-12-02T14:38:44ZCRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci10.1038/s41467-019-13403-y2041-1723https://doaj.org/article/e256e98fa6a7453b8ef036e4dd9081b92019-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-019-13403-yhttps://doaj.org/toc/2041-1723Inducible genome editing systems often suffer from leakiness or reduced activity. Here the authors develop CRISPR-Switch, a Cre recombinase ON/OFF-controlled sgRNA cassette that allows consecutive editing of two loci.Krzysztof ChylinskiMaria HubmannRuth E. HannaConnor YanchusGeorg MichlitsEsther C. H. UijttewaalJohn DoenchDaniel SchramekUlrich EllingNature PortfolioarticleScienceQENNature Communications, Vol 10, Iss 1, Pp 1-12 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Krzysztof Chylinski Maria Hubmann Ruth E. Hanna Connor Yanchus Georg Michlits Esther C. H. Uijttewaal John Doench Daniel Schramek Ulrich Elling CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
description	Inducible genome editing systems often suffer from leakiness or reduced activity. Here the authors develop CRISPR-Switch, a Cre recombinase ON/OFF-controlled sgRNA cassette that allows consecutive editing of two loci.
format	article
author	Krzysztof Chylinski Maria Hubmann Ruth E. Hanna Connor Yanchus Georg Michlits Esther C. H. Uijttewaal John Doench Daniel Schramek Ulrich Elling
author_facet	Krzysztof Chylinski Maria Hubmann Ruth E. Hanna Connor Yanchus Georg Michlits Esther C. H. Uijttewaal John Doench Daniel Schramek Ulrich Elling
author_sort	Krzysztof Chylinski
title	CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
title_short	CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
title_full	CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
title_fullStr	CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
title_full_unstemmed	CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci
title_sort	crispr-switch regulates sgrna activity by cre recombination for sequential editing of two loci
publisher	Nature Portfolio
publishDate	2019
url	https://doaj.org/article/e256e98fa6a7453b8ef036e4dd9081b9
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CRISPR-Switch regulates sgRNA activity by Cre recombination for sequential editing of two loci

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