Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.

Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cell...

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Autores principales:	Olga Antsiferova, Anne Müller, Patrick C Rämer, Obinna Chijioke, Bithi Chatterjee, Ana Raykova, Raquel Planas, Mireia Sospedra, Anatoliy Shumilov, Ming-Han Tsai, Henri-Jacques Delecluse, Christian Münz
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e25caa9499d24c2d95619727227fe222
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spelling	oai:doaj.org-article:e25caa9499d24c2d95619727227fe2222021-11-25T05:46:08ZAdoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.1553-73661553-737410.1371/journal.ppat.1004333https://doaj.org/article/e25caa9499d24c2d95619727227fe2222014-08-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/25165855/?tool=EBIhttps://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cells remains unclear. Here we demonstrate that lytic EBV replication does not significantly contribute to virus-induced B cell proliferation in vitro and in vivo in a mouse model with reconstituted human immune system components (huNSG mice). However, we report a trend to reduction of EBV-induced lymphoproliferation outside of lymphoid organs upon diminished lytic replication. Moreover, we could demonstrate that CD8+ T cells against the lytic EBV antigen BMLF1 can eliminate lytically replicating EBV-transformed B cells from lymphoblastoid cell lines (LCLs) and in vivo, thereby transiently controlling high viremia after adoptive transfer into EBV infected huNSG mice. These findings suggest a protective function for lytic EBV antigen-specific CD8+ T cells against EBV infection and against virus-associated tumors in extra-lymphoid organs. These specificities should be explored for EBV-specific vaccine development.Olga AntsiferovaAnne MüllerPatrick C RämerObinna ChijiokeBithi ChatterjeeAna RaykovaRaquel PlanasMireia SospedraAnatoliy ShumilovMing-Han TsaiHenri-Jacques DelecluseChristian MünzPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 10, Iss 8, p e1004333 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
description	Epstein Barr virus (EBV) infection expands CD8+ T cells specific for lytic antigens to high frequencies during symptomatic primary infection, and maintains these at significant numbers during persistence. Despite this, the protective function of these lytic EBV antigen-specific cytotoxic CD8+ T cells remains unclear. Here we demonstrate that lytic EBV replication does not significantly contribute to virus-induced B cell proliferation in vitro and in vivo in a mouse model with reconstituted human immune system components (huNSG mice). However, we report a trend to reduction of EBV-induced lymphoproliferation outside of lymphoid organs upon diminished lytic replication. Moreover, we could demonstrate that CD8+ T cells against the lytic EBV antigen BMLF1 can eliminate lytically replicating EBV-transformed B cells from lymphoblastoid cell lines (LCLs) and in vivo, thereby transiently controlling high viremia after adoptive transfer into EBV infected huNSG mice. These findings suggest a protective function for lytic EBV antigen-specific CD8+ T cells against EBV infection and against virus-associated tumors in extra-lymphoid organs. These specificities should be explored for EBV-specific vaccine development.
format	article
author	Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz
author_facet	Olga Antsiferova Anne Müller Patrick C Rämer Obinna Chijioke Bithi Chatterjee Ana Raykova Raquel Planas Mireia Sospedra Anatoliy Shumilov Ming-Han Tsai Henri-Jacques Delecluse Christian Münz
author_sort	Olga Antsiferova
title	Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
title_short	Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
title_full	Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
title_fullStr	Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
title_full_unstemmed	Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.
title_sort	adoptive transfer of ebv specific cd8+ t cell clones can transiently control ebv infection in humanized mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e25caa9499d24c2d95619727227fe222
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Adoptive transfer of EBV specific CD8+ T cell clones can transiently control EBV infection in humanized mice.

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