Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota

Abstract Daikenchuto (DKT) is a Japanese traditional herbal (Kampo) medicine containing ginseng, processed ginger, and Japanese or Chinese pepper. We aimed to determine how DKT affects human colonic microbiota. An in vitro microbiota model was established using fecal inocula collected from nine heal...

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Autores principales:	Kengo Sasaki, Daisuke Sasaki, Katsunori Sasaki, Yuto Nishidono, Akihiro Yamamori, Ken Tanaka, Akihiko Kondo
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e26380f2dca04cc094b9a7b231294925
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spelling	oai:doaj.org-article:e26380f2dca04cc094b9a7b2312949252021-12-02T13:20:12ZGrowth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota10.1038/s41598-021-84167-z2045-2322https://doaj.org/article/e26380f2dca04cc094b9a7b2312949252021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84167-zhttps://doaj.org/toc/2045-2322Abstract Daikenchuto (DKT) is a Japanese traditional herbal (Kampo) medicine containing ginseng, processed ginger, and Japanese or Chinese pepper. We aimed to determine how DKT affects human colonic microbiota. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. DKT was added to the in vitro microbiota model culture at a concentration of 0.5% by weight. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Additionally, in pure cultures, B. adolescentis transformed ginsenoside Rc to Rd, which was then probably utilized for its growth. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon.Kengo SasakiDaisuke SasakiKatsunori SasakiYuto NishidonoAkihiro YamamoriKen TanakaAkihiko KondoNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kengo Sasaki Daisuke Sasaki Katsunori Sasaki Yuto Nishidono Akihiro Yamamori Ken Tanaka Akihiko Kondo Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
description	Abstract Daikenchuto (DKT) is a Japanese traditional herbal (Kampo) medicine containing ginseng, processed ginger, and Japanese or Chinese pepper. We aimed to determine how DKT affects human colonic microbiota. An in vitro microbiota model was established using fecal inocula collected from nine healthy volunteers, and each model was found to retain operational taxonomic units similar to the ones in the original human fecal samples. DKT was added to the in vitro microbiota model culture at a concentration of 0.5% by weight. Next-generation sequencing of bacterial 16S rRNA gene revealed a significant increase in the relative abundance of bacteria related to the Bifidobacterium genus in the model after incubation with DKT. In pure cultures, DKT significantly promoted the growth of Bifidobacterium adolescentis, but not that of Fusobacterium nucleatum or Escherichia coli. Additionally, in pure cultures, B. adolescentis transformed ginsenoside Rc to Rd, which was then probably utilized for its growth. Our study reveals the in vitro bifidogenic effect of DKT that likely contributes to its beneficial effects on the human colon.
format	article
author	Kengo Sasaki Daisuke Sasaki Katsunori Sasaki Yuto Nishidono Akihiro Yamamori Ken Tanaka Akihiko Kondo
author_facet	Kengo Sasaki Daisuke Sasaki Katsunori Sasaki Yuto Nishidono Akihiro Yamamori Ken Tanaka Akihiko Kondo
author_sort	Kengo Sasaki
title	Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
title_short	Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
title_full	Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
title_fullStr	Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
title_full_unstemmed	Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
title_sort	growth stimulation of bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e26380f2dca04cc094b9a7b231294925
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Growth stimulation of Bifidobacterium from human colon using daikenchuto in an in vitro model of human intestinal microbiota

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