The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.

Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident th...

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Autores principales:	Barbara Hubad, Aleš Lapanje
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e265ede55811470a91e9620a8868019b
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spelling	oai:doaj.org-article:e265ede55811470a91e9620a8868019b2021-11-18T08:41:02ZThe efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.1932-620310.1371/journal.pone.0082186https://doaj.org/article/e265ede55811470a91e9620a8868019b2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24376520/?tool=EBIhttps://doaj.org/toc/1932-6203Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring.Barbara HubadAleš LapanjePublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e82186 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Barbara Hubad Aleš Lapanje The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
description	Cultivation-based microbiological methods are a gold standard for monitoring of airborne micro-organisms to determine the occupational exposure levels or transmission paths of a particular infectious agent. Some highly contagious microorganisms are not easily culturable but it is becoming evident that cultivation and molecular methods are complementary and in these cases highly relevant. We report a simple and efficient method for sampling and analyzing airborne bacteria with an impactor-type high-flow-rate portable air sampler, currently used for monitoring culturable bacteria or fungi. A method is reported for extraction of nucleic acids from impacted cells without prior cultivation and using agarose as a sampling matrix. The DNA extraction efficiency was determined in spiked samples and, samples taken from a wastewater treatment plant and an alpine area. The abundance, diversity and quantity of total bacteria were analysed by a quantitative polymerase chain reaction, and by construction and analysis of clone libraries. The method does not interfere with downstream PCR analysis and can cover the gap between traditional culture and molecular techniques of bioaerosol monitoring.
format	article
author	Barbara Hubad Aleš Lapanje
author_facet	Barbara Hubad Aleš Lapanje
author_sort	Barbara Hubad
title	The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
title_short	The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
title_full	The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
title_fullStr	The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
title_full_unstemmed	The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
title_sort	efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e265ede55811470a91e9620a8868019b
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The efficient method for simultaneous monitoring of the culturable as well as nonculturable airborne microorganisms.

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