Three-dimensional imaging of xylem at cell wall level through near field nano holotomography

Abstract Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) ima...

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Autores principales:	Tim Koddenberg, Imke Greving, Johannes Hagemann, Silja Flenner, Andreas Krause, Daniel Laipple, Kim C. Klein, Uwe Schmitt, Max Schuster, Andreas Wolf, Maria Seifert, Veronika Ludwig, Stefan Funk, Holger Militz, Martin Nopens
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e268ee8dcf3c4df0b472bae781c7475f
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spelling	oai:doaj.org-article:e268ee8dcf3c4df0b472bae781c7475f2021-12-02T15:54:01ZThree-dimensional imaging of xylem at cell wall level through near field nano holotomography10.1038/s41598-021-83885-82045-2322https://doaj.org/article/e268ee8dcf3c4df0b472bae781c7475f2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83885-8https://doaj.org/toc/2045-2322Abstract Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) imaging method for studying the hydraulic function of wooden plants. However, this X-ray imaging method can barely reach the resolution needed to see the minute structures (e.g. pit membrane). To complement the xylem structure with 3D views at the nanoscale level, X-ray near-field nano-holotomography (NFH) was applied to analyze the wood species Pinus sylvestris and Fagus sylvatica. The demanded small specimens required focused ion beam (FIB) application. The FIB milling, however, influenced the image quality through gallium implantation on the cell-wall surfaces. The measurements indicated that NFH is appropriate for imaging wood at nanometric resolution. With a 26 nm voxel pitch, the structure of the cell-wall surface in Pinus sylvestris could be visualized in genuine detail. In wood of Fagus sylvatica, the structure of a pit pair, including the pit membrane, between two neighboring fibrous cells could be traced tomographically.Tim KoddenbergImke GrevingJohannes HagemannSilja FlennerAndreas KrauseDaniel LaippleKim C. KleinUwe SchmittMax SchusterAndreas WolfMaria SeifertVeronika LudwigStefan FunkHolger MilitzMartin NopensNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-7 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Tim Koddenberg Imke Greving Johannes Hagemann Silja Flenner Andreas Krause Daniel Laipple Kim C. Klein Uwe Schmitt Max Schuster Andreas Wolf Maria Seifert Veronika Ludwig Stefan Funk Holger Militz Martin Nopens Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
description	Abstract Detailed imaging of the three-dimensionally complex architecture of xylary plants is important for studying biological and mechanical functions of woody plants. Apart from common two-dimensional microscopy, X-ray micro-computed tomography has been established as a three-dimensional (3D) imaging method for studying the hydraulic function of wooden plants. However, this X-ray imaging method can barely reach the resolution needed to see the minute structures (e.g. pit membrane). To complement the xylem structure with 3D views at the nanoscale level, X-ray near-field nano-holotomography (NFH) was applied to analyze the wood species Pinus sylvestris and Fagus sylvatica. The demanded small specimens required focused ion beam (FIB) application. The FIB milling, however, influenced the image quality through gallium implantation on the cell-wall surfaces. The measurements indicated that NFH is appropriate for imaging wood at nanometric resolution. With a 26 nm voxel pitch, the structure of the cell-wall surface in Pinus sylvestris could be visualized in genuine detail. In wood of Fagus sylvatica, the structure of a pit pair, including the pit membrane, between two neighboring fibrous cells could be traced tomographically.
format	article
author	Tim Koddenberg Imke Greving Johannes Hagemann Silja Flenner Andreas Krause Daniel Laipple Kim C. Klein Uwe Schmitt Max Schuster Andreas Wolf Maria Seifert Veronika Ludwig Stefan Funk Holger Militz Martin Nopens
author_facet	Tim Koddenberg Imke Greving Johannes Hagemann Silja Flenner Andreas Krause Daniel Laipple Kim C. Klein Uwe Schmitt Max Schuster Andreas Wolf Maria Seifert Veronika Ludwig Stefan Funk Holger Militz Martin Nopens
author_sort	Tim Koddenberg
title	Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
title_short	Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
title_full	Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
title_fullStr	Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
title_full_unstemmed	Three-dimensional imaging of xylem at cell wall level through near field nano holotomography
title_sort	three-dimensional imaging of xylem at cell wall level through near field nano holotomography
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e268ee8dcf3c4df0b472bae781c7475f
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Three-dimensional imaging of xylem at cell wall level through near field nano holotomography

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