A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR

In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11...

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Autores principales: Simeunović Predrag, Stevanović Jevrosima, Vidanović Dejan, Nišavić Jakov, Radović Dejan, Stanišić Ljubodrag, Stanimirović Zoran
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Lenguaje:EN
Publicado: Sciendo 2014
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Acceso en línea:https://doaj.org/article/e2694a2c301f44b8a1467f2db5c2e102
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spelling oai:doaj.org-article:e2694a2c301f44b8a1467f2db5c2e1022021-11-17T21:27:52ZA survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR1820-744810.2478/acve-2014-0009https://doaj.org/article/e2694a2c301f44b8a1467f2db5c2e1022014-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2014-0009https://doaj.org/toc/1820-7448In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.Simeunović PredragStevanović JevrosimaVidanović DejanNišavić JakovRadović DejanStanišić LjubodragStanimirović ZoranSciendoarticledwvabpvvarroa destructorserbian apiariesVeterinary medicineSF600-1100ENActa Veterinaria, Vol 64, Iss 1, Pp 81-92 (2014)
institution DOAJ
collection DOAJ
language EN
topic dwv
abpv
varroa destructor
serbian apiaries
Veterinary medicine
SF600-1100
spellingShingle dwv
abpv
varroa destructor
serbian apiaries
Veterinary medicine
SF600-1100
Simeunović Predrag
Stevanović Jevrosima
Vidanović Dejan
Nišavić Jakov
Radović Dejan
Stanišić Ljubodrag
Stanimirović Zoran
A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
description In this study 55 honey bee colonies from different Serbian regions were monitored for the presence of Deformed Wing Virus (DWV) and Acute Bee Paralysis Virus (ABPV) using TaqMan-based real-time RT-PCR assay. The results revealed the presence of DWV in each sampling location, and ABPV in 10 out of 11 apiaries. High frequency of DWV (76.4%) and ABPV (61.8%) positive samples in asymptomatic colonies can be the consequence of inefficient and postponed Varroa treatment concerning the role of this mite in the transmission and activation of honey bee viruses. The real-time RTPCR technique described in this paper is proved to be the most reliable method for this kind of investigation.
format article
author Simeunović Predrag
Stevanović Jevrosima
Vidanović Dejan
Nišavić Jakov
Radović Dejan
Stanišić Ljubodrag
Stanimirović Zoran
author_facet Simeunović Predrag
Stevanović Jevrosima
Vidanović Dejan
Nišavić Jakov
Radović Dejan
Stanišić Ljubodrag
Stanimirović Zoran
author_sort Simeunović Predrag
title A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
title_short A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
title_full A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
title_fullStr A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
title_full_unstemmed A survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time RT-PCR
title_sort survey of deformed wing virus and acute bee paralysis virus in honey bee colonies from serbia using real-time rt-pcr
publisher Sciendo
publishDate 2014
url https://doaj.org/article/e2694a2c301f44b8a1467f2db5c2e102
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