Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)

BACKGROUND AND OBJECTIVE: Klebsiella pneumoniae is one of the most important agents of nosocomial infections and drug resistance. Integrons are genetic elements that carry genetic determinants for antimicrobial drug resistance. The role of class I integron genes is very important to transfer of anti...

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Autores principales:	Z Molana, E Ferdosi Shahandashti, S Gharavi, M Shafii, S Norkhomami, F Ahangarkani, R Rajabnia
Formato:	article
Lenguaje:	EN FA
Publicado:	Babol University of Medical Sciences 2011
Materias:	klebsiella pneumoniae integron class i antibiotic resistance Medicine R Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e26b2d59fcf043e5a46d44be8f55998c
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spelling	oai:doaj.org-article:e26b2d59fcf043e5a46d44be8f55998c2021-11-10T08:56:57ZMolecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)1561-41072251-7170https://doaj.org/article/e26b2d59fcf043e5a46d44be8f55998c2011-11-01T00:00:00Zhttp://jbums.org/article-1-3919-en.htmlhttps://doaj.org/toc/1561-4107https://doaj.org/toc/2251-7170BACKGROUND AND OBJECTIVE: Klebsiella pneumoniae is one of the most important agents of nosocomial infections and drug resistance. Integrons are genetic elements that carry genetic determinants for antimicrobial drug resistance. The role of class I integron genes is very important to transfer of antibiotic resistance. The aim of the present study is isolation of Klebsiella pneumoniae from the environment and equipments in intensive care unit (ICU), investigation of class I integron genes in these strains and determine their susceptibility or resistance to antibiotics.METHODS: The descriptive and analytical study was carried out on 30 Klebsiella pneumoniae isolatesd from ICU of Shahid Beheshti hospital in Babol, Iran in 2010. Sampling of environment and equipments in ICU was carried out. After isolation and extraction of DNA, class I integron gene was investigated by PCR and specific primers. Also for the sensitivity test to antibiotics of Ciprofloxacin, Cefazolin, Ceftriaxone, Ceftizoxime, Cefotaxime, Amikacin, Ofloxazin, Imipenem, Cefepim, Ticarcilin and Gentamicin was performed by disk diffusion method.FINDINGS: From 30 Klebsiella pneumoniae isolates, 36.6 percent (11 samples) have had class I integron gene. After sensitivity test, from 9 of the 26 resistant isolates to Cefazolin, 10 of 25 resistant isolates to Ceftriaxone, 11 of 27 resistant isolates to Ceftizoxime, Cefotaxime and Cefepim, 9 of 18 resistant isolates to Ciprofloxacin and Imipenem, 11 of 26 resistant isolates to Ticarcilin, 5 of 17 resistant isolates to Ofloxazin, 5 of 11 resistant isolates to Amikacin and 8 of 26 resistant isolates to Gentamicin had class I integron gene. CONCLUSION: The results of this study show contamination of environment and equipments in ICU was increased. The class I integron gene frequency in these strains is high that can have important and basic role in occurrence and transfer of antibiotic resistance. The antibiotic resistance of Klebsiella pneumoniae, isolated from the environment and ICU equipments, is very high that it can be due to the irregular use of antibiotics. Measures should be thought to prevent occurrence and dissemination of antibiotic resistance elements.Z Molana,E Ferdosi Shahandashti,S Gharavi,M Shafii,S Norkhomami,F AhangarkaniR RajabniaBabol University of Medical Sciencesarticleklebsiella pneumoniaeintegron class iantibiotic resistanceMedicineRMedicine (General)R5-920ENFAMajallah-i Dānishgāh-i ̒Ulūm-i Pizishkī-i Bābul, Vol 13, Iss 6, Pp 7-13 (2011)
institution	DOAJ
collection	DOAJ
language	EN FA
topic	klebsiella pneumoniae integron class i antibiotic resistance Medicine R Medicine (General) R5-920
spellingShingle	klebsiella pneumoniae integron class i antibiotic resistance Medicine R Medicine (General) R5-920 Z Molana, E Ferdosi Shahandashti, S Gharavi, M Shafii, S Norkhomami, F Ahangarkani R Rajabnia Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
description	BACKGROUND AND OBJECTIVE: Klebsiella pneumoniae is one of the most important agents of nosocomial infections and drug resistance. Integrons are genetic elements that carry genetic determinants for antimicrobial drug resistance. The role of class I integron genes is very important to transfer of antibiotic resistance. The aim of the present study is isolation of Klebsiella pneumoniae from the environment and equipments in intensive care unit (ICU), investigation of class I integron genes in these strains and determine their susceptibility or resistance to antibiotics.METHODS: The descriptive and analytical study was carried out on 30 Klebsiella pneumoniae isolatesd from ICU of Shahid Beheshti hospital in Babol, Iran in 2010. Sampling of environment and equipments in ICU was carried out. After isolation and extraction of DNA, class I integron gene was investigated by PCR and specific primers. Also for the sensitivity test to antibiotics of Ciprofloxacin, Cefazolin, Ceftriaxone, Ceftizoxime, Cefotaxime, Amikacin, Ofloxazin, Imipenem, Cefepim, Ticarcilin and Gentamicin was performed by disk diffusion method.FINDINGS: From 30 Klebsiella pneumoniae isolates, 36.6 percent (11 samples) have had class I integron gene. After sensitivity test, from 9 of the 26 resistant isolates to Cefazolin, 10 of 25 resistant isolates to Ceftriaxone, 11 of 27 resistant isolates to Ceftizoxime, Cefotaxime and Cefepim, 9 of 18 resistant isolates to Ciprofloxacin and Imipenem, 11 of 26 resistant isolates to Ticarcilin, 5 of 17 resistant isolates to Ofloxazin, 5 of 11 resistant isolates to Amikacin and 8 of 26 resistant isolates to Gentamicin had class I integron gene. CONCLUSION: The results of this study show contamination of environment and equipments in ICU was increased. The class I integron gene frequency in these strains is high that can have important and basic role in occurrence and transfer of antibiotic resistance. The antibiotic resistance of Klebsiella pneumoniae, isolated from the environment and ICU equipments, is very high that it can be due to the irregular use of antibiotics. Measures should be thought to prevent occurrence and dissemination of antibiotic resistance elements.
format	article
author	Z Molana, E Ferdosi Shahandashti, S Gharavi, M Shafii, S Norkhomami, F Ahangarkani R Rajabnia
author_facet	Z Molana, E Ferdosi Shahandashti, S Gharavi, M Shafii, S Norkhomami, F Ahangarkani R Rajabnia
author_sort	Z Molana,
title	Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
title_short	Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
title_full	Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
title_fullStr	Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
title_full_unstemmed	Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)
title_sort	molecular investigation of class i integron in klebsiella pneumoniae isolated from intensive care unit (shahid beheshti hospital of babol 2010)
publisher	Babol University of Medical Sciences
publishDate	2011
url	https://doaj.org/article/e26b2d59fcf043e5a46d44be8f55998c
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Molecular Investigation of Class I Integron in Klebsiella Pneumoniae Isolated from Intensive Care Unit (Shahid Beheshti Hospital of Babol 2010)

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