Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

The SARS-CoV-2 viral genome is encapsulated by the nucleocapsid protein (NSARS-CoV-2) that is essential for viral replication. Here, the authors show that RNA induces liquid-liquid phase separation of NSARS-CoV-2 and how NSARS-CoV-2 phosphorylation modulates RNA-binding and phase separation and that...

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Autores principales:	Adriana Savastano, Alain Ibáñez de Opakua, Marija Rankovic, Markus Zweckstetter
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e27a188206ec47e389da49aa732648b3
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spelling	oai:doaj.org-article:e27a188206ec47e389da49aa732648b32021-12-02T16:49:28ZNucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates10.1038/s41467-020-19843-12041-1723https://doaj.org/article/e27a188206ec47e389da49aa732648b32020-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-19843-1https://doaj.org/toc/2041-1723The SARS-CoV-2 viral genome is encapsulated by the nucleocapsid protein (NSARS-CoV-2) that is essential for viral replication. Here, the authors show that RNA induces liquid-liquid phase separation of NSARS-CoV-2 and how NSARS-CoV-2 phosphorylation modulates RNA-binding and phase separation and that these RNA/NSARS-CoV-2-droplets recruit and concentrate the SARS-CoV-2 RNA-dependent RNA polymerase complex in vitro, which would enable high initiation and elongation rates during viral transcription.Adriana SavastanoAlain Ibáñez de OpakuaMarija RankovicMarkus ZweckstetterNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-10 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Adriana Savastano Alain Ibáñez de Opakua Marija Rankovic Markus Zweckstetter Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
description	The SARS-CoV-2 viral genome is encapsulated by the nucleocapsid protein (NSARS-CoV-2) that is essential for viral replication. Here, the authors show that RNA induces liquid-liquid phase separation of NSARS-CoV-2 and how NSARS-CoV-2 phosphorylation modulates RNA-binding and phase separation and that these RNA/NSARS-CoV-2-droplets recruit and concentrate the SARS-CoV-2 RNA-dependent RNA polymerase complex in vitro, which would enable high initiation and elongation rates during viral transcription.
format	article
author	Adriana Savastano Alain Ibáñez de Opakua Marija Rankovic Markus Zweckstetter
author_facet	Adriana Savastano Alain Ibáñez de Opakua Marija Rankovic Markus Zweckstetter
author_sort	Adriana Savastano
title	Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
title_short	Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
title_full	Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
title_fullStr	Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
title_full_unstemmed	Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates
title_sort	nucleocapsid protein of sars-cov-2 phase separates into rna-rich polymerase-containing condensates
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e27a188206ec47e389da49aa732648b3
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Nucleocapsid protein of SARS-CoV-2 phase separates into RNA-rich polymerase-containing condensates

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