Uptake of lymphoma-derived exosomes by peripheral blood leukocytes

Heather R Ferguson Bennit,1,2 Amber Gonda,1,3 Laura J Oppegard,2 David P Chi,2 Salma Khan,1,2 Nathan R Wall1,2 1Center for Health Disparities & Molecular Medicine, 2Division of Biochemistry, Department of Basic Sciences, 3Department of Anatomy, Loma Linda University School of Medicine, Loma...

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Autores principales:	Ferguson Bennit HR, Gonda A, Oppegard LJ, Chi DP, Khan S, Wall NR
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	exosome non-Hodgkin’s lymphoma B cell Diseases of the blood and blood-forming organs RC633-647.5
Acceso en línea:	https://doaj.org/article/e27be11b6e8a4394aa98420de140a8dc
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spelling	oai:doaj.org-article:e27be11b6e8a4394aa98420de140a8dc2021-12-02T02:29:11ZUptake of lymphoma-derived exosomes by peripheral blood leukocytes1179-9889https://doaj.org/article/e27be11b6e8a4394aa98420de140a8dc2017-02-01T00:00:00Zhttps://www.dovepress.com/uptake-of-lymphoma-derived-exosomes-by-peripheral-blood-leukocytes-peer-reviewed-article-BLCTThttps://doaj.org/toc/1179-9889Heather R Ferguson Bennit,1,2 Amber Gonda,1,3 Laura J Oppegard,2 David P Chi,2 Salma Khan,1,2 Nathan R Wall1,2 1Center for Health Disparities & Molecular Medicine, 2Division of Biochemistry, Department of Basic Sciences, 3Department of Anatomy, Loma Linda University School of Medicine, Loma Linda, CA, USA Abstract: Exosomes are nanosized lipid vesicles secreted into blood and other body fluids and serve as vehicles for intercellular communication. Despite being an important component of the tumor microenvironment (TME), exosomal targeting and uptake into recipient cells are still not fully understood. Few studies have looked at lymphoma exosomes and their interactions with circulating blood cells. In this study, we examine the exosomal uptake distribution among peripheral blood leukocytes (PBLs) using vesicles derived from a diffuse large B cell lymphoma cell line, WSU-DLCL2. Lymphoma cells survive, proliferate, and are protected from the cytotoxic effects of chemotherapeutic agents by soluble factors or by direct contact with inflammatory and stromal cells within the TME. In an attempt to close the gap in knowledge concerning lymphoma TME immunosuppression, we have treated normal human PBLs with PKH67-labeled lymphoma exosomes and monitored the uptake by measuring fluorescence at different time points using flow cytometry and fluorescent microscopy. Our results show that of the four populations examined, B cells and monocytes demonstrated uptake of PKH67-labeled exosomes, while T cells and NK cells displayed significantly less uptake. Keywords: exosome, non-Hodgkin’s lymphoma, B cellFerguson Bennit HRGonda AOppegard LJChi DPKhan SWall NRDove Medical Pressarticleexosomenon-Hodgkin’s lymphomaB cellDiseases of the blood and blood-forming organsRC633-647.5ENBlood and Lymphatic Cancer: Targets and Therapy, Vol Volume 7, Pp 9-23 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosome non-Hodgkin’s lymphoma B cell Diseases of the blood and blood-forming organs RC633-647.5
spellingShingle	exosome non-Hodgkin’s lymphoma B cell Diseases of the blood and blood-forming organs RC633-647.5 Ferguson Bennit HR Gonda A Oppegard LJ Chi DP Khan S Wall NR Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
description	Heather R Ferguson Bennit,1,2 Amber Gonda,1,3 Laura J Oppegard,2 David P Chi,2 Salma Khan,1,2 Nathan R Wall1,2 1Center for Health Disparities & Molecular Medicine, 2Division of Biochemistry, Department of Basic Sciences, 3Department of Anatomy, Loma Linda University School of Medicine, Loma Linda, CA, USA Abstract: Exosomes are nanosized lipid vesicles secreted into blood and other body fluids and serve as vehicles for intercellular communication. Despite being an important component of the tumor microenvironment (TME), exosomal targeting and uptake into recipient cells are still not fully understood. Few studies have looked at lymphoma exosomes and their interactions with circulating blood cells. In this study, we examine the exosomal uptake distribution among peripheral blood leukocytes (PBLs) using vesicles derived from a diffuse large B cell lymphoma cell line, WSU-DLCL2. Lymphoma cells survive, proliferate, and are protected from the cytotoxic effects of chemotherapeutic agents by soluble factors or by direct contact with inflammatory and stromal cells within the TME. In an attempt to close the gap in knowledge concerning lymphoma TME immunosuppression, we have treated normal human PBLs with PKH67-labeled lymphoma exosomes and monitored the uptake by measuring fluorescence at different time points using flow cytometry and fluorescent microscopy. Our results show that of the four populations examined, B cells and monocytes demonstrated uptake of PKH67-labeled exosomes, while T cells and NK cells displayed significantly less uptake. Keywords: exosome, non-Hodgkin’s lymphoma, B cell
format	article
author	Ferguson Bennit HR Gonda A Oppegard LJ Chi DP Khan S Wall NR
author_facet	Ferguson Bennit HR Gonda A Oppegard LJ Chi DP Khan S Wall NR
author_sort	Ferguson Bennit HR
title	Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
title_short	Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
title_full	Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
title_fullStr	Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
title_full_unstemmed	Uptake of lymphoma-derived exosomes by peripheral blood leukocytes
title_sort	uptake of lymphoma-derived exosomes by peripheral blood leukocytes
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/e27be11b6e8a4394aa98420de140a8dc
work_keys_str_mv	AT fergusonbennithr uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes AT gondaa uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes AT oppegardlj uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes AT chidp uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes AT khans uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes AT wallnr uptakeoflymphomaderivedexosomesbyperipheralbloodleukocytes
_version_	1718402500193681408

Uptake of lymphoma-derived exosomes by peripheral blood leukocytes

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