Cellular localization and processing of primary transcripts of exonic microRNAs.

Processing of miRNAs occurs simultaneous with the transcription and splicing of their primary transcripts. For the small subset of exonic miRNAs it is unclear if the unspliced and/or spliced transcripts are used for miRNA biogenesis. We assessed endogenous levels and cellular location of primary tra...

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Autores principales:	Izabella Slezak-Prochazka, Joost Kluiver, Debora de Jong, Gertrud Kortman, Nancy Halsema, Sibrand Poppema, Bart-Jan Kroesen, Anke van den Berg
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2874de8a1604f51aa25bdf687459001
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spelling	oai:doaj.org-article:e2874de8a1604f51aa25bdf6874590012021-11-18T08:54:14ZCellular localization and processing of primary transcripts of exonic microRNAs.1932-620310.1371/journal.pone.0076647https://doaj.org/article/e2874de8a1604f51aa25bdf6874590012013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24073292/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Processing of miRNAs occurs simultaneous with the transcription and splicing of their primary transcripts. For the small subset of exonic miRNAs it is unclear if the unspliced and/or spliced transcripts are used for miRNA biogenesis. We assessed endogenous levels and cellular location of primary transcripts of three exonic miRNAs. The ratio between unspliced and spliced transcripts varied markedly, i.e. >1 for BIC, <1 for pri-miR-146a and variable for pri-miR-22. Endogenous unspliced transcripts were located almost exclusively in the nucleus and thus available for miRNA processing for all three miRNAs. Endogenous spliced pri-miRNA transcripts were present both in the nucleus and in the cytoplasm and thus only partly available for miRNA processing. Overexpression of constructs containing the 5' upstream exonic or intronic sequence flanking pre-miR-155 resulted in strongly enhanced miR-155 levels, indicating that the flanking sequence does not affect processing efficiency. Exogenously overexpressed full-length spliced BIC transcripts were present both in the nucleus and in the cytoplasm, were bound by the Microprocessor complex and resulted in enhanced miR-155 levels. We conclude that both unspliced and spliced transcripts of exonic miRNAs can be used for pre-miRNA cleavage. Splicing and cytoplasmic transport of spliced transcripts may present a mechanism to regulate levels of exonic microRNAs.Izabella Slezak-ProchazkaJoost KluiverDebora de JongGertrud KortmanNancy HalsemaSibrand PoppemaBart-Jan KroesenAnke van den BergPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 9, p e76647 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Izabella Slezak-Prochazka Joost Kluiver Debora de Jong Gertrud Kortman Nancy Halsema Sibrand Poppema Bart-Jan Kroesen Anke van den Berg Cellular localization and processing of primary transcripts of exonic microRNAs.
description	Processing of miRNAs occurs simultaneous with the transcription and splicing of their primary transcripts. For the small subset of exonic miRNAs it is unclear if the unspliced and/or spliced transcripts are used for miRNA biogenesis. We assessed endogenous levels and cellular location of primary transcripts of three exonic miRNAs. The ratio between unspliced and spliced transcripts varied markedly, i.e. >1 for BIC, <1 for pri-miR-146a and variable for pri-miR-22. Endogenous unspliced transcripts were located almost exclusively in the nucleus and thus available for miRNA processing for all three miRNAs. Endogenous spliced pri-miRNA transcripts were present both in the nucleus and in the cytoplasm and thus only partly available for miRNA processing. Overexpression of constructs containing the 5' upstream exonic or intronic sequence flanking pre-miR-155 resulted in strongly enhanced miR-155 levels, indicating that the flanking sequence does not affect processing efficiency. Exogenously overexpressed full-length spliced BIC transcripts were present both in the nucleus and in the cytoplasm, were bound by the Microprocessor complex and resulted in enhanced miR-155 levels. We conclude that both unspliced and spliced transcripts of exonic miRNAs can be used for pre-miRNA cleavage. Splicing and cytoplasmic transport of spliced transcripts may present a mechanism to regulate levels of exonic microRNAs.
format	article
author	Izabella Slezak-Prochazka Joost Kluiver Debora de Jong Gertrud Kortman Nancy Halsema Sibrand Poppema Bart-Jan Kroesen Anke van den Berg
author_facet	Izabella Slezak-Prochazka Joost Kluiver Debora de Jong Gertrud Kortman Nancy Halsema Sibrand Poppema Bart-Jan Kroesen Anke van den Berg
author_sort	Izabella Slezak-Prochazka
title	Cellular localization and processing of primary transcripts of exonic microRNAs.
title_short	Cellular localization and processing of primary transcripts of exonic microRNAs.
title_full	Cellular localization and processing of primary transcripts of exonic microRNAs.
title_fullStr	Cellular localization and processing of primary transcripts of exonic microRNAs.
title_full_unstemmed	Cellular localization and processing of primary transcripts of exonic microRNAs.
title_sort	cellular localization and processing of primary transcripts of exonic micrornas.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/e2874de8a1604f51aa25bdf687459001
work_keys_str_mv	AT izabellaslezakprochazka cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT joostkluiver cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT deboradejong cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT gertrudkortman cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT nancyhalsema cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT sibrandpoppema cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT bartjankroesen cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas AT ankevandenberg cellularlocalizationandprocessingofprimarytranscriptsofexonicmicrornas
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Cellular localization and processing of primary transcripts of exonic microRNAs.

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