A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces

Abstract Listeria monocytogenes is a foodborne pathogen responsible for human listeriosis. The increasing incidence of listeriosis induced governments and food manufacturing enterprises to act to diminish the problem. Several methods for the detection of Listeria monocytogenes in food industries wer...

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Autores principales:	Alessandro Capo, Sabato D’Auria, Monique Lacroix
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2a24932ace74d93914ff7dd5380544d
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spelling	oai:doaj.org-article:e2a24932ace74d93914ff7dd5380544d2021-12-02T12:33:14ZA fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces10.1038/s41598-020-77747-y2045-2322https://doaj.org/article/e2a24932ace74d93914ff7dd5380544d2020-12-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-77747-yhttps://doaj.org/toc/2045-2322Abstract Listeria monocytogenes is a foodborne pathogen responsible for human listeriosis. The increasing incidence of listeriosis induced governments and food manufacturing enterprises to act to diminish the problem. Several methods for the detection of Listeria monocytogenes in food industries were developed. However, they are time-consuming and require the use of specialized equipment. To reduce the detection time of Listeria monocytogenes in food, in this work we developed a fluorescence sandwich immunoassay based on the use of an innovative chitosan-cellulose nanocrystal (CNC) membrane that improves the antigen capture during bacterial growth. The combined use of CNC film for the capture of p60 protein-specific antigen together with the use of fluorescence detection reduced the time of analysis from 24 to 12 h with a limit of detection (LOD) of the assay of 102 CFU/mL (2 Log). In addition, the use of monoclonal anti-PepD covalently immobilized to a CNC membrane assured a high specificity of the assay. Interestingly, the obtained results show no cross-reactivity with the five most diffused pathogen bacteria strains tested.Alessandro CapoSabato D’AuriaMonique LacroixNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-12 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alessandro Capo Sabato D’Auria Monique Lacroix A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
description	Abstract Listeria monocytogenes is a foodborne pathogen responsible for human listeriosis. The increasing incidence of listeriosis induced governments and food manufacturing enterprises to act to diminish the problem. Several methods for the detection of Listeria monocytogenes in food industries were developed. However, they are time-consuming and require the use of specialized equipment. To reduce the detection time of Listeria monocytogenes in food, in this work we developed a fluorescence sandwich immunoassay based on the use of an innovative chitosan-cellulose nanocrystal (CNC) membrane that improves the antigen capture during bacterial growth. The combined use of CNC film for the capture of p60 protein-specific antigen together with the use of fluorescence detection reduced the time of analysis from 24 to 12 h with a limit of detection (LOD) of the assay of 102 CFU/mL (2 Log). In addition, the use of monoclonal anti-PepD covalently immobilized to a CNC membrane assured a high specificity of the assay. Interestingly, the obtained results show no cross-reactivity with the five most diffused pathogen bacteria strains tested.
format	article
author	Alessandro Capo Sabato D’Auria Monique Lacroix
author_facet	Alessandro Capo Sabato D’Auria Monique Lacroix
author_sort	Alessandro Capo
title	A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
title_short	A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
title_full	A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
title_fullStr	A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
title_full_unstemmed	A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces
title_sort	fluorescence immunoassay for a rapid detection of listeria monocytogenes on working surfaces
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e2a24932ace74d93914ff7dd5380544d
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A fluorescence immunoassay for a rapid detection of Listeria monocytogenes on working surfaces

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