Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen Pseudomonas syringae pv. actinidiae

Bacteriophages are viruses that specifically infect target bacteria. Recently, bacteriophages have been considered potential biological control agents for bacterial pathogens due to their host specificity. Pseudomonas syringae pv. actinidiae (Psa) is a reemergin...

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Autores principales:	Yanxi Liu, Mengjiao Liu, Ran Hu, Jun Bai, Xiaoqing He, Yi Jin
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	bacteriophage phage therapy <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> <i>Caudoviricetes</i> Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e2a8e32730d74dbe92eb657a555c9a6f
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spelling	oai:doaj.org-article:e2a8e32730d74dbe92eb657a555c9a6f2021-11-25T19:14:01ZIsolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>10.3390/v131122751999-4915https://doaj.org/article/e2a8e32730d74dbe92eb657a555c9a6f2021-11-01T00:00:00Zhttps://www.mdpi.com/1999-4915/13/11/2275https://doaj.org/toc/1999-4915Bacteriophages are viruses that specifically infect target bacteria. Recently, bacteriophages have been considered potential biological control agents for bacterial pathogens due to their host specificity. <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a reemerging pathogen that causes bacterial canker of kiwifruit (<i>Actinidia</i> sp.). The economic impact of this pest and the development of resistance to antibiotics and copper sprays in Psa and other pathovars have led to investigation of alternative management strategies. Phage therapy may be a useful alternative to conventional treatments for controlling Psa infections. Although the efficacy of bacteriophage φ6 was evaluated for the control of Psa, the characteristics of other DNA bacteriophages infecting Psa remain unclear. In this study, the PHB09 lytic bacteriophage specific to Psa was isolated from kiwifruit orchard soil. Extensive host range testing using Psa isolated from kiwifruit orchards and other <i>Pseudomonas</i> strains showed PHB09 has a narrow host range. It remained stable over a wide range of temperatures (4–50 °C) and pH values (pH 3–11) and maintained stability for 50 min under ultraviolet irradiation. Complete genome sequence analysis indicated PHB09 might belong to a new myovirus genus in <i>Caudoviricetes</i>. Its genome contains a total of 94,844 bp and 186 predicted genes associated with phage structure, packaging, host lysis, DNA manipulation, transcription, and additional functions. The isolation and identification of PHB09 enrich the research on <i>Pseudomonas</i> phages and provide a promising biocontrol agent against kiwifruit bacterial canker.Yanxi LiuMengjiao LiuRan HuJun BaiXiaoqing HeYi JinMDPI AGarticlebacteriophagephage therapy<i>Pseudomonas syringae</i> pv. <i>actinidiae</i><i>Caudoviricetes</i>MicrobiologyQR1-502ENViruses, Vol 13, Iss 2275, p 2275 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	bacteriophage phage therapy <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> <i>Caudoviricetes</i> Microbiology QR1-502
spellingShingle	bacteriophage phage therapy <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> <i>Caudoviricetes</i> Microbiology QR1-502 Yanxi Liu Mengjiao Liu Ran Hu Jun Bai Xiaoqing He Yi Jin Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
description	Bacteriophages are viruses that specifically infect target bacteria. Recently, bacteriophages have been considered potential biological control agents for bacterial pathogens due to their host specificity. <i>Pseudomonas syringae</i> pv. <i>actinidiae</i> (Psa) is a reemerging pathogen that causes bacterial canker of kiwifruit (<i>Actinidia</i> sp.). The economic impact of this pest and the development of resistance to antibiotics and copper sprays in Psa and other pathovars have led to investigation of alternative management strategies. Phage therapy may be a useful alternative to conventional treatments for controlling Psa infections. Although the efficacy of bacteriophage φ6 was evaluated for the control of Psa, the characteristics of other DNA bacteriophages infecting Psa remain unclear. In this study, the PHB09 lytic bacteriophage specific to Psa was isolated from kiwifruit orchard soil. Extensive host range testing using Psa isolated from kiwifruit orchards and other <i>Pseudomonas</i> strains showed PHB09 has a narrow host range. It remained stable over a wide range of temperatures (4–50 °C) and pH values (pH 3–11) and maintained stability for 50 min under ultraviolet irradiation. Complete genome sequence analysis indicated PHB09 might belong to a new myovirus genus in <i>Caudoviricetes</i>. Its genome contains a total of 94,844 bp and 186 predicted genes associated with phage structure, packaging, host lysis, DNA manipulation, transcription, and additional functions. The isolation and identification of PHB09 enrich the research on <i>Pseudomonas</i> phages and provide a promising biocontrol agent against kiwifruit bacterial canker.
format	article
author	Yanxi Liu Mengjiao Liu Ran Hu Jun Bai Xiaoqing He Yi Jin
author_facet	Yanxi Liu Mengjiao Liu Ran Hu Jun Bai Xiaoqing He Yi Jin
author_sort	Yanxi Liu
title	Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
title_short	Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
title_full	Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
title_fullStr	Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
title_full_unstemmed	Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>
title_sort	isolation of the novel phage phb09 and its potential use against the plant pathogen <i>pseudomonas syringae</i> pv. <i>actinidiae</i>
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e2a8e32730d74dbe92eb657a555c9a6f
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Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen <i>Pseudomonas syringae</i> pv. <i>actinidiae</i>

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