Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.

Lepidopteran insect pests are the main class of pests causing significant damage to crop plant yields. Insecticidal scorpion peptides exhibit toxicity specific for insects. Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xiuzi Tianpei, Yingguo Zhu, Shaoqing Li
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2aa3510d8a04ff39719a39e39171b12
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e2aa3510d8a04ff39719a39e39171b12
record_format	dspace
spelling	oai:doaj.org-article:e2aa3510d8a04ff39719a39e39171b122021-11-11T08:21:34ZOptimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.1932-620310.1371/journal.pone.0100232https://doaj.org/article/e2aa3510d8a04ff39719a39e39171b122014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24964088/?tool=EBIhttps://doaj.org/toc/1932-6203Lepidopteran insect pests are the main class of pests causing significant damage to crop plant yields. Insecticidal scorpion peptides exhibit toxicity specific for insects. Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activity against rice leaf folder in vitro and in planta. Oral ingestion of LMX protein led to a significant decrease in feeding on rice leaves, repression of larval growth and development, delay in molting, and increase in larval lethality. Compared with LqhIT2 protein, the stability and insecticidal efficacy of LMX was better. Meanwhile, biochemical analysis showed that LMX protein ingestion dramatically decreased ecdysone content in rice leaf folder larvae, and down-regulated enzymatic activities of the detoxification system (α-naphthyl acetate esterase and glutathione S-transferase), the digestive system (tryptase and chymotrypsin), and the antioxidant system (catalase). These changes were tightly correlated with the dosage of LMX protein. Transgene analysis showed that the rate of leaf damage, and the number of damaged tillers and leaves in the transgenic line were greatly reduced relative to wild type plants and empty vector plants. Based on these observations, we propose that the insect-specific scorpion neurotoxin peptide LMX is an attractive and effective alternative molecule for the protection of rice from rice leaf folder.Xiuzi TianpeiYingguo ZhuShaoqing LiPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100232 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Xiuzi Tianpei Yingguo Zhu Shaoqing Li Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
description	Lepidopteran insect pests are the main class of pests causing significant damage to crop plant yields. Insecticidal scorpion peptides exhibit toxicity specific for insects. Here, we report that a peptide LMX, optimized from the insect-specific scorpion neurotoxin LqhIT2, showed high levels of activity against rice leaf folder in vitro and in planta. Oral ingestion of LMX protein led to a significant decrease in feeding on rice leaves, repression of larval growth and development, delay in molting, and increase in larval lethality. Compared with LqhIT2 protein, the stability and insecticidal efficacy of LMX was better. Meanwhile, biochemical analysis showed that LMX protein ingestion dramatically decreased ecdysone content in rice leaf folder larvae, and down-regulated enzymatic activities of the detoxification system (α-naphthyl acetate esterase and glutathione S-transferase), the digestive system (tryptase and chymotrypsin), and the antioxidant system (catalase). These changes were tightly correlated with the dosage of LMX protein. Transgene analysis showed that the rate of leaf damage, and the number of damaged tillers and leaves in the transgenic line were greatly reduced relative to wild type plants and empty vector plants. Based on these observations, we propose that the insect-specific scorpion neurotoxin peptide LMX is an attractive and effective alternative molecule for the protection of rice from rice leaf folder.
format	article
author	Xiuzi Tianpei Yingguo Zhu Shaoqing Li
author_facet	Xiuzi Tianpei Yingguo Zhu Shaoqing Li
author_sort	Xiuzi Tianpei
title	Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
title_short	Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
title_full	Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
title_fullStr	Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
title_full_unstemmed	Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.
title_sort	optimized scorpion polypeptide lmx: a pest control protein effective against rice leaf folder.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/e2aa3510d8a04ff39719a39e39171b12
work_keys_str_mv	AT xiuzitianpei optimizedscorpionpolypeptidelmxapestcontrolproteineffectiveagainstriceleaffolder AT yingguozhu optimizedscorpionpolypeptidelmxapestcontrolproteineffectiveagainstriceleaffolder AT shaoqingli optimizedscorpionpolypeptidelmxapestcontrolproteineffectiveagainstriceleaffolder
_version_	1718439358648811520

Optimized scorpion polypeptide LMX: a pest control protein effective against rice leaf folder.

Ejemplares similares