Persistence and accumulation of environmental DNA from an endangered dragonfly

Abstract Detection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, most studies in this field favor vertebrate target species. Environmental DNA protocols can be especially useful...

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Autores principales:	Kristie J. Schmidt, Daniel A. Soluk, Sarah E. Mays Maestas, Hugh B. Britten
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2ac96b30ab04bb785219a369d1c37e2
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spelling	oai:doaj.org-article:e2ac96b30ab04bb785219a369d1c37e22021-12-02T18:14:39ZPersistence and accumulation of environmental DNA from an endangered dragonfly10.1038/s41598-021-98099-12045-2322https://doaj.org/article/e2ac96b30ab04bb785219a369d1c37e22021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-98099-1https://doaj.org/toc/2045-2322Abstract Detection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, most studies in this field favor vertebrate target species. Environmental DNA protocols can be especially useful for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is to evaluate the dynamics of eDNA concentration under controlled conditions. We used the quantitative polymerase chain reaction (qPCR) to examine seasonal shifts in the persistence and net-accumulation of eDNA from captive S. hineana larvae in experimental mesocosms at temperatures corresponding with their overwintering (5.0 °C) and active (16.0 °C) seasons. Environmental DNA persisted longer at 5.0 °C but accumulated more readily at 16.0 °C. Differences in the accumulation and persistence of eDNA reflect differences in the longevity of eDNA at different temperatures and seasonal differences in larval S. hineana behavior. This study highlights the importance of considering how seasonal changes in temperature influence not only the speed of eDNA degradation but also the target species’ eDNA shedding rates.Kristie J. SchmidtDaniel A. SolukSarah E. Mays MaestasHugh B. BrittenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Kristie J. Schmidt Daniel A. Soluk Sarah E. Mays Maestas Hugh B. Britten Persistence and accumulation of environmental DNA from an endangered dragonfly
description	Abstract Detection of environmental DNA (eDNA) has become a commonly used surveillance method for threatened or invasive vertebrates in both aquatic and terrestrial environments. However, most studies in this field favor vertebrate target species. Environmental DNA protocols can be especially useful for endangered invertebrates such as the Hine’s emerald dragonfly (Somatochlora hineana) where conservation efforts have been greatly hindered by training, time, overall costs, and environmental impacts associated with conducting surveys in the calcareous fens occupied by this species. An essential step in developing such a protocol is to evaluate the dynamics of eDNA concentration under controlled conditions. We used the quantitative polymerase chain reaction (qPCR) to examine seasonal shifts in the persistence and net-accumulation of eDNA from captive S. hineana larvae in experimental mesocosms at temperatures corresponding with their overwintering (5.0 °C) and active (16.0 °C) seasons. Environmental DNA persisted longer at 5.0 °C but accumulated more readily at 16.0 °C. Differences in the accumulation and persistence of eDNA reflect differences in the longevity of eDNA at different temperatures and seasonal differences in larval S. hineana behavior. This study highlights the importance of considering how seasonal changes in temperature influence not only the speed of eDNA degradation but also the target species’ eDNA shedding rates.
format	article
author	Kristie J. Schmidt Daniel A. Soluk Sarah E. Mays Maestas Hugh B. Britten
author_facet	Kristie J. Schmidt Daniel A. Soluk Sarah E. Mays Maestas Hugh B. Britten
author_sort	Kristie J. Schmidt
title	Persistence and accumulation of environmental DNA from an endangered dragonfly
title_short	Persistence and accumulation of environmental DNA from an endangered dragonfly
title_full	Persistence and accumulation of environmental DNA from an endangered dragonfly
title_fullStr	Persistence and accumulation of environmental DNA from an endangered dragonfly
title_full_unstemmed	Persistence and accumulation of environmental DNA from an endangered dragonfly
title_sort	persistence and accumulation of environmental dna from an endangered dragonfly
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e2ac96b30ab04bb785219a369d1c37e2
work_keys_str_mv	AT kristiejschmidt persistenceandaccumulationofenvironmentaldnafromanendangereddragonfly AT danielasoluk persistenceandaccumulationofenvironmentaldnafromanendangereddragonfly AT sarahemaysmaestas persistenceandaccumulationofenvironmentaldnafromanendangereddragonfly AT hughbbritten persistenceandaccumulationofenvironmentaldnafromanendangereddragonfly
_version_	1718378427315126272

Persistence and accumulation of environmental DNA from an endangered dragonfly

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