Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma

Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer. Approximately 80% of patients initially diagnosed with locally advanced or metastatic disease survive only 4–11 months after diagnosis. Tremendous efforts have been made toward understanding the biology of PDAC. Results...

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Autores principales:	Hongjin Wu, Weiwei Tian, Xiang Tai, Xuanpeng Li, Ziwei Li, Jing Shui, Juehua Yu, Zhihua Wang, Xiaosong Zhu
Formato:	article
Lenguaje:	EN
Publicado:	BMC 2021
Materias:	Pancreatic ductal adenocarcinoma (PDAC) Oncogene DDX60L MYEOV CXCL2 Biotechnology TP248.13-248.65 Genetics QH426-470
Acceso en línea:	https://doaj.org/article/e2ad0849a2ad476fb109ff4b4278cb8f
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spelling	oai:doaj.org-article:e2ad0849a2ad476fb109ff4b4278cb8f2021-11-21T12:26:29ZIdentification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma10.1186/s12864-021-08137-51471-2164https://doaj.org/article/e2ad0849a2ad476fb109ff4b4278cb8f2021-11-01T00:00:00Zhttps://doi.org/10.1186/s12864-021-08137-5https://doaj.org/toc/1471-2164Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer. Approximately 80% of patients initially diagnosed with locally advanced or metastatic disease survive only 4–11 months after diagnosis. Tremendous efforts have been made toward understanding the biology of PDAC. Results In this study, we first utilized next-generation sequencing technique and existing microarray datasets to identify significant differentially expressed genes between PDAC and non-tumor adjacent tissue. By comparing top significant survival genes in PDAC Gene Expression Profiling Interactive Analysis database and PDAC transcriptome data from patients, our integrated analysis discovered five potential central genes (i.e., MYEOV, KCNN4, FAM83A, S100A16, and DDX60L). Subsequently, we analyzed the cellular functions of the potential novel oncogenes MYEOV and DDX60L, which are highly expressed in PDAC cells. Notably, the knockdown of MYEOV and DDX60L significantly inhibited the metastasis of cancer cells and induced apoptosis. Further RNA sequencing analyses showed that massive signaling pathways, particularly the TNF signaling pathway and nuclear factor-kappa B (NF-κB) signaling pathway, were affected in siRNA-treated cancer cells. The siDDX60L and siMYEOV significantly inhibited the expression of chemokine CXCL2, which may potentially affect the tumor microenvironment in PDAC tissues. Conclusions The present findings identified the novel oncogene DDX60L, which was highly expressed in PDAC. Transcriptome profiling through siRNA knockdown of DDX60L uncovered its functional roles in the PDAC in humans.Hongjin WuWeiwei TianXiang TaiXuanpeng LiZiwei LiJing ShuiJuehua YuZhihua WangXiaosong ZhuBMCarticlePancreatic ductal adenocarcinoma (PDAC)OncogeneDDX60LMYEOVCXCL2BiotechnologyTP248.13-248.65GeneticsQH426-470ENBMC Genomics, Vol 22, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Pancreatic ductal adenocarcinoma (PDAC) Oncogene DDX60L MYEOV CXCL2 Biotechnology TP248.13-248.65 Genetics QH426-470
spellingShingle	Pancreatic ductal adenocarcinoma (PDAC) Oncogene DDX60L MYEOV CXCL2 Biotechnology TP248.13-248.65 Genetics QH426-470 Hongjin Wu Weiwei Tian Xiang Tai Xuanpeng Li Ziwei Li Jing Shui Juehua Yu Zhihua Wang Xiaosong Zhu Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
description	Abstract Background Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer. Approximately 80% of patients initially diagnosed with locally advanced or metastatic disease survive only 4–11 months after diagnosis. Tremendous efforts have been made toward understanding the biology of PDAC. Results In this study, we first utilized next-generation sequencing technique and existing microarray datasets to identify significant differentially expressed genes between PDAC and non-tumor adjacent tissue. By comparing top significant survival genes in PDAC Gene Expression Profiling Interactive Analysis database and PDAC transcriptome data from patients, our integrated analysis discovered five potential central genes (i.e., MYEOV, KCNN4, FAM83A, S100A16, and DDX60L). Subsequently, we analyzed the cellular functions of the potential novel oncogenes MYEOV and DDX60L, which are highly expressed in PDAC cells. Notably, the knockdown of MYEOV and DDX60L significantly inhibited the metastasis of cancer cells and induced apoptosis. Further RNA sequencing analyses showed that massive signaling pathways, particularly the TNF signaling pathway and nuclear factor-kappa B (NF-κB) signaling pathway, were affected in siRNA-treated cancer cells. The siDDX60L and siMYEOV significantly inhibited the expression of chemokine CXCL2, which may potentially affect the tumor microenvironment in PDAC tissues. Conclusions The present findings identified the novel oncogene DDX60L, which was highly expressed in PDAC. Transcriptome profiling through siRNA knockdown of DDX60L uncovered its functional roles in the PDAC in humans.
format	article
author	Hongjin Wu Weiwei Tian Xiang Tai Xuanpeng Li Ziwei Li Jing Shui Juehua Yu Zhihua Wang Xiaosong Zhu
author_facet	Hongjin Wu Weiwei Tian Xiang Tai Xuanpeng Li Ziwei Li Jing Shui Juehua Yu Zhihua Wang Xiaosong Zhu
author_sort	Hongjin Wu
title	Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
title_short	Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
title_full	Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
title_fullStr	Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
title_full_unstemmed	Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma
title_sort	identification and functional analysis of novel oncogene ddx60l in pancreatic ductal adenocarcinoma
publisher	BMC
publishDate	2021
url	https://doaj.org/article/e2ad0849a2ad476fb109ff4b4278cb8f
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_version_	1718419036514025472

Identification and functional analysis of novel oncogene DDX60L in pancreatic ductal adenocarcinoma

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