Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

Autologous tumor cells and cell-derived secretions (CDS) can induce antitumor immune responses. The conditions in which cells are cultured and treated impact CDS, and cellular insults alter their composition and function. In this study, we generated CDS from tumor cells exposed to normal culture con...

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Autores principales:	Yuhan Zhou, Ting Ye, Chengzhi Ye, Chao Wan, Siyue Yuan, Yushuai Liu, Tianyu Li, Fagang Jiang, Jonathan F. Lovell, Honglin Jin, Jing Chen
Formato:	article
Lenguaje:	EN
Publicado:	KeAi Communications Co., Ltd. 2022
Materias:	Cancer immunotherapy Hydrogel Melittin Anti-PD-1 Cell-derived secretions Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/e2b468649da94e4c8b60ddbf32767fd5
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spelling	oai:doaj.org-article:e2b468649da94e4c8b60ddbf32767fd52021-11-10T04:30:43ZSecretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy2452-199X10.1016/j.bioactmat.2021.07.019https://doaj.org/article/e2b468649da94e4c8b60ddbf32767fd52022-03-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2452199X21003510https://doaj.org/toc/2452-199XAutologous tumor cells and cell-derived secretions (CDS) can induce antitumor immune responses. The conditions in which cells are cultured and treated impact CDS, and cellular insults alter their composition and function. In this study, we generated CDS from tumor cells exposed to normal culture conditions, hypoxia, cisplatin, radiotherapy, photodynamic therapy, or hypochlorous acid (HOCl). In vitro HOCl-CDS showed the strongest stimulatory effects on dendritic cells and macrophages compared to CDS generated by hypoxia, cisplatin, radiotherapy or photodynamic therapy. To improve HOCl-CDS activity at the tumor site, we loaded HOCl-CDS into a melittin-encapsulated hydrogel scaffold. When injected intratumorally, the HOCl-CDS hydrogel promoted tumor cell death, cytotoxic T lymphocyte infiltration, and tumor-associated macrophage reprogramming towards an M1 phenotype. The hydrogel inhibited tumor growth and prolonged the survival of mice bearing B16–F10 melanoma. Furthermore, hydrogel-delivered HOCl-CDS augmented the antitumor effects of immune checkpoint blockade. These results underscore the importance of the CDS generation method and delivery approach for improving cancer immunotherapy.Yuhan ZhouTing YeChengzhi YeChao WanSiyue YuanYushuai LiuTianyu LiFagang JiangJonathan F. LovellHonglin JinJing ChenKeAi Communications Co., Ltd.articleCancer immunotherapyHydrogelMelittinAnti-PD-1Cell-derived secretionsMaterials of engineering and construction. Mechanics of materialsTA401-492Biology (General)QH301-705.5ENBioactive Materials, Vol 9, Iss , Pp 541-553 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cancer immunotherapy Hydrogel Melittin Anti-PD-1 Cell-derived secretions Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5
spellingShingle	Cancer immunotherapy Hydrogel Melittin Anti-PD-1 Cell-derived secretions Materials of engineering and construction. Mechanics of materials TA401-492 Biology (General) QH301-705.5 Yuhan Zhou Ting Ye Chengzhi Ye Chao Wan Siyue Yuan Yushuai Liu Tianyu Li Fagang Jiang Jonathan F. Lovell Honglin Jin Jing Chen Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
description	Autologous tumor cells and cell-derived secretions (CDS) can induce antitumor immune responses. The conditions in which cells are cultured and treated impact CDS, and cellular insults alter their composition and function. In this study, we generated CDS from tumor cells exposed to normal culture conditions, hypoxia, cisplatin, radiotherapy, photodynamic therapy, or hypochlorous acid (HOCl). In vitro HOCl-CDS showed the strongest stimulatory effects on dendritic cells and macrophages compared to CDS generated by hypoxia, cisplatin, radiotherapy or photodynamic therapy. To improve HOCl-CDS activity at the tumor site, we loaded HOCl-CDS into a melittin-encapsulated hydrogel scaffold. When injected intratumorally, the HOCl-CDS hydrogel promoted tumor cell death, cytotoxic T lymphocyte infiltration, and tumor-associated macrophage reprogramming towards an M1 phenotype. The hydrogel inhibited tumor growth and prolonged the survival of mice bearing B16–F10 melanoma. Furthermore, hydrogel-delivered HOCl-CDS augmented the antitumor effects of immune checkpoint blockade. These results underscore the importance of the CDS generation method and delivery approach for improving cancer immunotherapy.
format	article
author	Yuhan Zhou Ting Ye Chengzhi Ye Chao Wan Siyue Yuan Yushuai Liu Tianyu Li Fagang Jiang Jonathan F. Lovell Honglin Jin Jing Chen
author_facet	Yuhan Zhou Ting Ye Chengzhi Ye Chao Wan Siyue Yuan Yushuai Liu Tianyu Li Fagang Jiang Jonathan F. Lovell Honglin Jin Jing Chen
author_sort	Yuhan Zhou
title	Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
title_short	Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
title_full	Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
title_fullStr	Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
title_full_unstemmed	Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
title_sort	secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy
publisher	KeAi Communications Co., Ltd.
publishDate	2022
url	https://doaj.org/article/e2b468649da94e4c8b60ddbf32767fd5
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Secretions from hypochlorous acid-treated tumor cells delivered in a melittin hydrogel potentiate cancer immunotherapy

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