Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins

Ribosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-f...

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Autores principales:	Bernardo Bonilauri, Fabiola Barbieri Holetz, Bruno Dallagiovanna
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	lncRNA ribosome smORF microprotein translation stem cells Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/e2b71f07175c4e8ea4c0a12f0a7da7d9
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spelling	oai:doaj.org-article:e2b71f07175c4e8ea4c0a12f0a7da7d92021-11-25T16:53:38ZLong Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins10.3390/biom111116732218-273Xhttps://doaj.org/article/e2b71f07175c4e8ea4c0a12f0a7da7d92021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1673https://doaj.org/toc/2218-273XRibosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-fide translated smORFs is a constant challenge in experimental and bioinformatics fields due to their unconventional characteristics. This motivated us to isolate human adipose-derived stem cells (hASC) from adipose tissue and perform a ribosome profiling followed by bioinformatics analysis of transcriptome, translatome, and ribosome-protected fragments of lncRNAs. Here, we demonstrated that 222 lncRNAs were associated with the translational machinery in hASC, including the already demonstrated lncRNAs coding microproteins. The ribosomal occupancy of some transcripts was consistent with the translation of smORFs. In conclusion, we were able to identify a subset of 15 lncRNAs containing 35 smORFs that likely encode functional microproteins, including four previously demonstrated smORF-derived microproteins, suggesting a possible dual role of these lncRNAs in hASC self-renewal.Bernardo BonilauriFabiola Barbieri HoletzBruno DallagiovannaMDPI AGarticlelncRNAribosomesmORFmicroproteintranslationstem cellsMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1673, p 1673 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	lncRNA ribosome smORF microprotein translation stem cells Microbiology QR1-502
spellingShingle	lncRNA ribosome smORF microprotein translation stem cells Microbiology QR1-502 Bernardo Bonilauri Fabiola Barbieri Holetz Bruno Dallagiovanna Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
description	Ribosome profiling reveals the translational dynamics of mRNAs by capturing a ribosomal footprint snapshot. Growing evidence shows that several long non-coding RNAs (lncRNAs) contain small open reading frames (smORFs) that are translated into functional peptides. The difficulty in identifying bona-fide translated smORFs is a constant challenge in experimental and bioinformatics fields due to their unconventional characteristics. This motivated us to isolate human adipose-derived stem cells (hASC) from adipose tissue and perform a ribosome profiling followed by bioinformatics analysis of transcriptome, translatome, and ribosome-protected fragments of lncRNAs. Here, we demonstrated that 222 lncRNAs were associated with the translational machinery in hASC, including the already demonstrated lncRNAs coding microproteins. The ribosomal occupancy of some transcripts was consistent with the translation of smORFs. In conclusion, we were able to identify a subset of 15 lncRNAs containing 35 smORFs that likely encode functional microproteins, including four previously demonstrated smORF-derived microproteins, suggesting a possible dual role of these lncRNAs in hASC self-renewal.
format	article
author	Bernardo Bonilauri Fabiola Barbieri Holetz Bruno Dallagiovanna
author_facet	Bernardo Bonilauri Fabiola Barbieri Holetz Bruno Dallagiovanna
author_sort	Bernardo Bonilauri
title	Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
title_short	Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
title_full	Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
title_fullStr	Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
title_full_unstemmed	Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins
title_sort	long non-coding rnas associated with ribosomes in human adipose-derived stem cells: from rnas to microproteins
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/e2b71f07175c4e8ea4c0a12f0a7da7d9
work_keys_str_mv	AT bernardobonilauri longnoncodingrnasassociatedwithribosomesinhumanadiposederivedstemcellsfromrnastomicroproteins AT fabiolabarbieriholetz longnoncodingrnasassociatedwithribosomesinhumanadiposederivedstemcellsfromrnastomicroproteins AT brunodallagiovanna longnoncodingrnasassociatedwithribosomesinhumanadiposederivedstemcellsfromrnastomicroproteins
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Long Non-Coding RNAs Associated with Ribosomes in Human Adipose-Derived Stem Cells: From RNAs to Microproteins

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