Expression of genes of the Pho regulon is altered in Streptomyces coelicolor

Abstract Most currently used antibiotics originate from Streptomycetes and phosphate limitation is an important trigger of their biosynthesis. Understanding the molecular processes underpinning such regulation is of crucial importance to exploit the great metabolic diversity of these bacteria and ge...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Aaron Millan-Oropeza, Céline Henry, Clara Lejeune, Michelle David, Marie-Joelle Virolle
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2c0106a316a46dd93eab1c581e541f1
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:e2c0106a316a46dd93eab1c581e541f1
record_format	dspace
spelling	oai:doaj.org-article:e2c0106a316a46dd93eab1c581e541f12021-12-02T14:58:45ZExpression of genes of the Pho regulon is altered in Streptomyces coelicolor10.1038/s41598-020-65087-w2045-2322https://doaj.org/article/e2c0106a316a46dd93eab1c581e541f12020-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-65087-whttps://doaj.org/toc/2045-2322Abstract Most currently used antibiotics originate from Streptomycetes and phosphate limitation is an important trigger of their biosynthesis. Understanding the molecular processes underpinning such regulation is of crucial importance to exploit the great metabolic diversity of these bacteria and get a better understanding of the role of these molecules in the physiology of the producing bacteria. To contribute to this field, a comparative proteomic analysis of two closely related model strains, Streptomyces lividans and Streptomyces coelicolor was carried out. These strains possess identical biosynthetic pathways directing the synthesis of three well-characterized antibiotics (CDA, RED and ACT) but only S. coelicolor expresses them at a high level. Previous studies established that the antibiotic producer, S. coelicolor, is characterized by an oxidative metabolism and a reduced triacylglycerol content compared to the none producer, S. lividans, characterized by a glycolytic metabolism. Our proteomic data support these findings and reveal that these drastically different metabolic features could, at least in part, due to the weaker abundance of proteins of the two component system PhoR/PhoP in S. coelicolor compared to S. lividans. In condition of phosphate limitation, PhoR/PhoP is known to control positively and negatively, respectively, phosphate and nitrogen assimilation and our study revealed that it might also control the expression of some genes of central carbon metabolism. The tuning down of the regulatory role of PhoR/PhoP in S. coelicolor is thus expected to be correlated with low and high phosphate and nitrogen availability, respectively and with changes in central carbon metabolic features. These changes are likely to be responsible for the observed differences between S. coelicolor and S. lividans concerning energetic metabolism, triacylglycerol biosynthesis and antibiotic production. Furthermore, a novel view of the contribution of the bio-active molecules produced in this context, to the regulation of the energetic metabolism of the producing bacteria, is proposed and discussed.Aaron Millan-OropezaCéline HenryClara LejeuneMichelle DavidMarie-Joelle VirolleNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 10, Iss 1, Pp 1-21 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Aaron Millan-Oropeza Céline Henry Clara Lejeune Michelle David Marie-Joelle Virolle Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
description	Abstract Most currently used antibiotics originate from Streptomycetes and phosphate limitation is an important trigger of their biosynthesis. Understanding the molecular processes underpinning such regulation is of crucial importance to exploit the great metabolic diversity of these bacteria and get a better understanding of the role of these molecules in the physiology of the producing bacteria. To contribute to this field, a comparative proteomic analysis of two closely related model strains, Streptomyces lividans and Streptomyces coelicolor was carried out. These strains possess identical biosynthetic pathways directing the synthesis of three well-characterized antibiotics (CDA, RED and ACT) but only S. coelicolor expresses them at a high level. Previous studies established that the antibiotic producer, S. coelicolor, is characterized by an oxidative metabolism and a reduced triacylglycerol content compared to the none producer, S. lividans, characterized by a glycolytic metabolism. Our proteomic data support these findings and reveal that these drastically different metabolic features could, at least in part, due to the weaker abundance of proteins of the two component system PhoR/PhoP in S. coelicolor compared to S. lividans. In condition of phosphate limitation, PhoR/PhoP is known to control positively and negatively, respectively, phosphate and nitrogen assimilation and our study revealed that it might also control the expression of some genes of central carbon metabolism. The tuning down of the regulatory role of PhoR/PhoP in S. coelicolor is thus expected to be correlated with low and high phosphate and nitrogen availability, respectively and with changes in central carbon metabolic features. These changes are likely to be responsible for the observed differences between S. coelicolor and S. lividans concerning energetic metabolism, triacylglycerol biosynthesis and antibiotic production. Furthermore, a novel view of the contribution of the bio-active molecules produced in this context, to the regulation of the energetic metabolism of the producing bacteria, is proposed and discussed.
format	article
author	Aaron Millan-Oropeza Céline Henry Clara Lejeune Michelle David Marie-Joelle Virolle
author_facet	Aaron Millan-Oropeza Céline Henry Clara Lejeune Michelle David Marie-Joelle Virolle
author_sort	Aaron Millan-Oropeza
title	Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
title_short	Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
title_full	Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
title_fullStr	Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
title_full_unstemmed	Expression of genes of the Pho regulon is altered in Streptomyces coelicolor
title_sort	expression of genes of the pho regulon is altered in streptomyces coelicolor
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/e2c0106a316a46dd93eab1c581e541f1
work_keys_str_mv	AT aaronmillanoropeza expressionofgenesofthephoregulonisalteredinstreptomycescoelicolor AT celinehenry expressionofgenesofthephoregulonisalteredinstreptomycescoelicolor AT claralejeune expressionofgenesofthephoregulonisalteredinstreptomycescoelicolor AT michelledavid expressionofgenesofthephoregulonisalteredinstreptomycescoelicolor AT mariejoellevirolle expressionofgenesofthephoregulonisalteredinstreptomycescoelicolor
_version_	1718389296432414720

Expression of genes of the Pho regulon is altered in Streptomyces coelicolor

Ejemplares similares