Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia

Abstract Preeclampsia (PE) is a prevalent pregnancy disorder that leads to high maternal and fetal morbidity and mortality. While defective vascular development and angiogenesis in placenta are known as crucial pathological findings, its pathophysiological mechanism remains elusive. To better unders...

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Autores principales:	Seonggeon Cho, Young-Doug Sohn, Sangsung Kim, Augustine Rajakumar, Martina L. Badell, Neil Sidell, Young-sup Yoon
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2c02648246749118589b17baac795b3
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spelling	oai:doaj.org-article:e2c02648246749118589b17baac795b32021-12-02T13:30:34ZReduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia10.1038/s41598-021-83146-82045-2322https://doaj.org/article/e2c02648246749118589b17baac795b32021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-83146-8https://doaj.org/toc/2045-2322Abstract Preeclampsia (PE) is a prevalent pregnancy disorder that leads to high maternal and fetal morbidity and mortality. While defective vascular development and angiogenesis in placenta are known as crucial pathological findings, its pathophysiological mechanism remains elusive. To better understand the effects of PE on angio-vasculogenesis and inflammatory networks in the fetus and to identify their biological signatures, we investigated the quantitative and functional characteristics of cord blood-derived mononuclear cells (CB-MNCs) and CD31-positive MNCs. Flow cytometry analysis demonstrated that the CB-MNCs from the severe PE group had significantly decreased number of cells expressing CD3, CD11b, CD14, CD19, KDR, and CD31 compared with the normal group. Quantitative real time PCR (qRT-PCR) shows down-regulation of the major angiogenic factor VEGFA in MNCs and CD31+ MNCs in severe PE. The major inflammatory cytokines IL1 was highly upregulated in CD31+ CB-MNCs in the severe PE patients. Mild PE patients, however, did not display any significant difference in expression of all measured angiogenic genes and most inflammatory genes. These findings show distinct angiogenic and inflammatory signatures from severe PE, and they may play a significant role in the pathogenesis of vascular defects in placenta of severe PE.Seonggeon ChoYoung-Doug SohnSangsung KimAugustine RajakumarMartina L. BadellNeil SidellYoung-sup YoonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Seonggeon Cho Young-Doug Sohn Sangsung Kim Augustine Rajakumar Martina L. Badell Neil Sidell Young-sup Yoon Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
description	Abstract Preeclampsia (PE) is a prevalent pregnancy disorder that leads to high maternal and fetal morbidity and mortality. While defective vascular development and angiogenesis in placenta are known as crucial pathological findings, its pathophysiological mechanism remains elusive. To better understand the effects of PE on angio-vasculogenesis and inflammatory networks in the fetus and to identify their biological signatures, we investigated the quantitative and functional characteristics of cord blood-derived mononuclear cells (CB-MNCs) and CD31-positive MNCs. Flow cytometry analysis demonstrated that the CB-MNCs from the severe PE group had significantly decreased number of cells expressing CD3, CD11b, CD14, CD19, KDR, and CD31 compared with the normal group. Quantitative real time PCR (qRT-PCR) shows down-regulation of the major angiogenic factor VEGFA in MNCs and CD31+ MNCs in severe PE. The major inflammatory cytokines IL1 was highly upregulated in CD31+ CB-MNCs in the severe PE patients. Mild PE patients, however, did not display any significant difference in expression of all measured angiogenic genes and most inflammatory genes. These findings show distinct angiogenic and inflammatory signatures from severe PE, and they may play a significant role in the pathogenesis of vascular defects in placenta of severe PE.
format	article
author	Seonggeon Cho Young-Doug Sohn Sangsung Kim Augustine Rajakumar Martina L. Badell Neil Sidell Young-sup Yoon
author_facet	Seonggeon Cho Young-Doug Sohn Sangsung Kim Augustine Rajakumar Martina L. Badell Neil Sidell Young-sup Yoon
author_sort	Seonggeon Cho
title	Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
title_short	Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
title_full	Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
title_fullStr	Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
title_full_unstemmed	Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
title_sort	reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e2c02648246749118589b17baac795b3
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Reduced angiovasculogenic and increased inflammatory profiles of cord blood cells in severe but not mild preeclampsia

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