Generation of functional ciliated cholangiocytes from human pluripotent stem cells

Current protocols to generate cholangiocytes from human pluripotent cells produce immature cells. Here the authors identify retinoic acid, BMP, cAMP and Rho kinase pathways as regulators of cholangiocyte maturation, and generate ciliated cholangiocytes expressing high levels of CFTR that form ductal...

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Autores principales:	Mina Ogawa, Jia-Xin Jiang, Sunny Xia, Donghe Yang, Avrilynn Ding, Onofrio Laselva, Marcela Hernandez, Changyi Cui, Yuichiro Higuchi, Hiroshi Suemizu, Craig Dorrell, Markus Grompe, Christine E. Bear, Shinichiro Ogawa
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/e2c37e554f8c4d829b4039f62abf625f
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spelling	oai:doaj.org-article:e2c37e554f8c4d829b4039f62abf625f2021-11-14T12:34:07ZGeneration of functional ciliated cholangiocytes from human pluripotent stem cells10.1038/s41467-021-26764-02041-1723https://doaj.org/article/e2c37e554f8c4d829b4039f62abf625f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-26764-0https://doaj.org/toc/2041-1723Current protocols to generate cholangiocytes from human pluripotent cells produce immature cells. Here the authors identify retinoic acid, BMP, cAMP and Rho kinase pathways as regulators of cholangiocyte maturation, and generate ciliated cholangiocytes expressing high levels of CFTR that form ductal structures in vivo.Mina OgawaJia-Xin JiangSunny XiaDonghe YangAvrilynn DingOnofrio LaselvaMarcela HernandezChangyi CuiYuichiro HiguchiHiroshi SuemizuCraig DorrellMarkus GrompeChristine E. BearShinichiro OgawaNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-19 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Mina Ogawa Jia-Xin Jiang Sunny Xia Donghe Yang Avrilynn Ding Onofrio Laselva Marcela Hernandez Changyi Cui Yuichiro Higuchi Hiroshi Suemizu Craig Dorrell Markus Grompe Christine E. Bear Shinichiro Ogawa Generation of functional ciliated cholangiocytes from human pluripotent stem cells
description	Current protocols to generate cholangiocytes from human pluripotent cells produce immature cells. Here the authors identify retinoic acid, BMP, cAMP and Rho kinase pathways as regulators of cholangiocyte maturation, and generate ciliated cholangiocytes expressing high levels of CFTR that form ductal structures in vivo.
format	article
author	Mina Ogawa Jia-Xin Jiang Sunny Xia Donghe Yang Avrilynn Ding Onofrio Laselva Marcela Hernandez Changyi Cui Yuichiro Higuchi Hiroshi Suemizu Craig Dorrell Markus Grompe Christine E. Bear Shinichiro Ogawa
author_facet	Mina Ogawa Jia-Xin Jiang Sunny Xia Donghe Yang Avrilynn Ding Onofrio Laselva Marcela Hernandez Changyi Cui Yuichiro Higuchi Hiroshi Suemizu Craig Dorrell Markus Grompe Christine E. Bear Shinichiro Ogawa
author_sort	Mina Ogawa
title	Generation of functional ciliated cholangiocytes from human pluripotent stem cells
title_short	Generation of functional ciliated cholangiocytes from human pluripotent stem cells
title_full	Generation of functional ciliated cholangiocytes from human pluripotent stem cells
title_fullStr	Generation of functional ciliated cholangiocytes from human pluripotent stem cells
title_full_unstemmed	Generation of functional ciliated cholangiocytes from human pluripotent stem cells
title_sort	generation of functional ciliated cholangiocytes from human pluripotent stem cells
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/e2c37e554f8c4d829b4039f62abf625f
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Generation of functional ciliated cholangiocytes from human pluripotent stem cells

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