Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions

Summary: Tight coordination between transcription and translation has long been recognized as the hallmark of gene expression in bacteria. In Escherichia coli cells, disruption of the transcription-translation coordination leads to the loss of transcription processivity via triggering Rho-mediated p...

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Autores principales:	Manlu Zhu, Haoyan Mu, Fei Han, Qian Wang, Xiongfeng Dai
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Biochemistry Bacteriology Synthetic biology Science Q
Acceso en línea:	https://doaj.org/article/e2c562a7311244b38fbd39391db36dea
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spelling	oai:doaj.org-article:e2c562a7311244b38fbd39391db36dea2021-11-20T05:10:04ZQuantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions2589-004210.1016/j.isci.2021.103333https://doaj.org/article/e2c562a7311244b38fbd39391db36dea2021-11-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S258900422101302Xhttps://doaj.org/toc/2589-0042Summary: Tight coordination between transcription and translation has long been recognized as the hallmark of gene expression in bacteria. In Escherichia coli cells, disruption of the transcription-translation coordination leads to the loss of transcription processivity via triggering Rho-mediated premature transcription termination. Here we quantitatively characterize the transcription and translation kinetics in Gram-positive model bacterium Bacillus subtilis. We found that the speed of transcription elongation is much faster than that of translation elongation in B. subtilis under various growth conditions. Moreover, a Rho-independent loss of transcription processivity occurs constitutively in several genes/operons but is not subject to translational control. When the transcription elongation is decelerated under poor nutrients, low temperature, or nucleotide depletion, the loss of transcription processivity is strongly enhanced, suggesting that its degree is modulated by the speed of transcription elongation. Our study reveals distinct design principles of gene expression in E. coli and B. subtilis.Manlu ZhuHaoyan MuFei HanQian WangXiongfeng DaiElsevierarticleBiochemistryBacteriologySynthetic biologyScienceQENiScience, Vol 24, Iss 11, Pp 103333- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Biochemistry Bacteriology Synthetic biology Science Q
spellingShingle	Biochemistry Bacteriology Synthetic biology Science Q Manlu Zhu Haoyan Mu Fei Han Qian Wang Xiongfeng Dai Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
description	Summary: Tight coordination between transcription and translation has long been recognized as the hallmark of gene expression in bacteria. In Escherichia coli cells, disruption of the transcription-translation coordination leads to the loss of transcription processivity via triggering Rho-mediated premature transcription termination. Here we quantitatively characterize the transcription and translation kinetics in Gram-positive model bacterium Bacillus subtilis. We found that the speed of transcription elongation is much faster than that of translation elongation in B. subtilis under various growth conditions. Moreover, a Rho-independent loss of transcription processivity occurs constitutively in several genes/operons but is not subject to translational control. When the transcription elongation is decelerated under poor nutrients, low temperature, or nucleotide depletion, the loss of transcription processivity is strongly enhanced, suggesting that its degree is modulated by the speed of transcription elongation. Our study reveals distinct design principles of gene expression in E. coli and B. subtilis.
format	article
author	Manlu Zhu Haoyan Mu Fei Han Qian Wang Xiongfeng Dai
author_facet	Manlu Zhu Haoyan Mu Fei Han Qian Wang Xiongfeng Dai
author_sort	Manlu Zhu
title	Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
title_short	Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
title_full	Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
title_fullStr	Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
title_full_unstemmed	Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions
title_sort	quantitative analysis of asynchronous transcription-translation and transcription processivity in bacillus subtilis under various growth conditions
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/e2c562a7311244b38fbd39391db36dea
work_keys_str_mv	AT manluzhu quantitativeanalysisofasynchronoustranscriptiontranslationandtranscriptionprocessivityinbacillussubtilisundervariousgrowthconditions AT haoyanmu quantitativeanalysisofasynchronoustranscriptiontranslationandtranscriptionprocessivityinbacillussubtilisundervariousgrowthconditions AT feihan quantitativeanalysisofasynchronoustranscriptiontranslationandtranscriptionprocessivityinbacillussubtilisundervariousgrowthconditions AT qianwang quantitativeanalysisofasynchronoustranscriptiontranslationandtranscriptionprocessivityinbacillussubtilisundervariousgrowthconditions AT xiongfengdai quantitativeanalysisofasynchronoustranscriptiontranslationandtranscriptionprocessivityinbacillussubtilisundervariousgrowthconditions
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Quantitative analysis of asynchronous transcription-translation and transcription processivity in Bacillus subtilis under various growth conditions

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