Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.

<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gen...

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Autores principales:	Rémy A Bonnin, Laurent Poirel, Alessandra Carattoli, Patrice Nordmann
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2ca0dab02ba4dc793840608adadb6ae
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spelling	oai:doaj.org-article:e2ca0dab02ba4dc793840608adadb6ae2021-11-18T07:22:25ZCharacterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.1932-620310.1371/journal.pone.0034752https://doaj.org/article/e2ca0dab02ba4dc793840608adadb6ae2012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22511964/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed.<h4>Principal findings</h4>Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene.<h4>Significance</h4>This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids.Rémy A BonninLaurent PoirelAlessandra CarattoliPatrice NordmannPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 4, p e34752 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
description	<h4>Background</h4>The current spread of the gene encoding the metallo-ß-lactamase NDM-1 in Enterobacteriaceae is linked to a variety of surrounding genetic structures and plasmid scaffolds.<h4>Methodology</h4>The whole sequence of plasmid pGUE-NDM carrying the bla(NDM-1) gene was determined by high-density pyrosequencing and a genomic comparative analysis with other bla(NDM-1)-negative IncFII was performed.<h4>Principal findings</h4>Plasmid pGUE-NDM replicating in Escherichia coli confers resistance to many antibiotic molecules including β-lactams, aminoglycosides, trimethoprim, and sulfonamides. It is 87,022 bp in-size and carries the two β-lactamase genes bla(NDM-1) and bla(OXA-1), together with three aminoglycoside resistance genes aacA4, aadA2, and aacC2. Comparative analysis of the multidrug resistance locus contained a module encompassing the bla(NDM-1) gene that is actually conserved among different structures identified in other enterobacterial isolates. This module was constituted by the bla(NDM-1) gene, a fragment of insertion sequence ISAba125 and a bleomycin resistance encoding gene.<h4>Significance</h4>This is the first characterized bla(NDM-1)-carrying IncFII-type plasmid. Such association between the bla(NDM-1) gene and an IncFII-type plasmid backbone is extremely worrisome considering that this plasmid type is known to spread efficiently, as examplified with the worldwide dissemination of bla(CTX-M-15)-borne IncFII plasmids.
format	article
author	Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann
author_facet	Rémy A Bonnin Laurent Poirel Alessandra Carattoli Patrice Nordmann
author_sort	Rémy A Bonnin
title	Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
title_short	Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
title_full	Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
title_fullStr	Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
title_full_unstemmed	Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.
title_sort	characterization of an incfii plasmid encoding ndm-1 from escherichia coli st131.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/e2ca0dab02ba4dc793840608adadb6ae
work_keys_str_mv	AT remyabonnin characterizationofanincfiiplasmidencodingndm1fromescherichiacolist131 AT laurentpoirel characterizationofanincfiiplasmidencodingndm1fromescherichiacolist131 AT alessandracarattoli characterizationofanincfiiplasmidencodingndm1fromescherichiacolist131 AT patricenordmann characterizationofanincfiiplasmidencodingndm1fromescherichiacolist131
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Characterization of an IncFII plasmid encoding NDM-1 from Escherichia coli ST131.

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