Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.

During embryonic vasculogenesis, endothelial precursor cells of mesodermal origin known as angioblasts assemble into a characteristic network pattern. Although a considerable amount of markers and signals involved in this process have been identified, the mechanisms underlying the coalescence of ang...

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Autores principales:	Alvaro Köhn-Luque, Walter de Back, Jörn Starruss, Andrea Mattiotti, Andreas Deutsch, José María Pérez-Pomares, Miguel A Herrero
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2d3b5533aaa492e91ae6cdc8021063b
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spelling	oai:doaj.org-article:e2d3b5533aaa492e91ae6cdc8021063b2021-11-04T06:08:14ZEarly embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.1932-620310.1371/journal.pone.0024175https://doaj.org/article/e2d3b5533aaa492e91ae6cdc8021063b2011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/21949696/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203During embryonic vasculogenesis, endothelial precursor cells of mesodermal origin known as angioblasts assemble into a characteristic network pattern. Although a considerable amount of markers and signals involved in this process have been identified, the mechanisms underlying the coalescence of angioblasts into this reticular pattern remain unclear. Various recent studies hypothesize that autocrine regulation of the chemoattractant vascular endothelial growth factor (VEGF) is responsible for the formation of vascular networks in vitro. However, the autocrine regulation hypothesis does not fit well with reported data on in vivo early vascular development. In this study, we propose a mathematical model based on the alternative assumption that endodermal VEGF signalling activity, having a paracrine effect on adjacent angioblasts, is mediated by its binding to the extracellular matrix (ECM). Detailed morphometric analysis of simulated networks and images obtained from in vivo quail embryos reveals the model mimics the vascular patterns with high accuracy. These results show that paracrine signalling can result in the formation of fine-grained cellular networks when mediated by angioblast-produced ECM. This lends additional support to the theory that patterning during early vascular development in the vertebrate embryo is regulated by paracrine signalling.Alvaro Köhn-LuqueWalter de BackJörn StarrussAndrea MattiottiAndreas DeutschJosé María Pérez-PomaresMiguel A HerreroPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 9, p e24175 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Alvaro Köhn-Luque Walter de Back Jörn Starruss Andrea Mattiotti Andreas Deutsch José María Pérez-Pomares Miguel A Herrero Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
description	During embryonic vasculogenesis, endothelial precursor cells of mesodermal origin known as angioblasts assemble into a characteristic network pattern. Although a considerable amount of markers and signals involved in this process have been identified, the mechanisms underlying the coalescence of angioblasts into this reticular pattern remain unclear. Various recent studies hypothesize that autocrine regulation of the chemoattractant vascular endothelial growth factor (VEGF) is responsible for the formation of vascular networks in vitro. However, the autocrine regulation hypothesis does not fit well with reported data on in vivo early vascular development. In this study, we propose a mathematical model based on the alternative assumption that endodermal VEGF signalling activity, having a paracrine effect on adjacent angioblasts, is mediated by its binding to the extracellular matrix (ECM). Detailed morphometric analysis of simulated networks and images obtained from in vivo quail embryos reveals the model mimics the vascular patterns with high accuracy. These results show that paracrine signalling can result in the formation of fine-grained cellular networks when mediated by angioblast-produced ECM. This lends additional support to the theory that patterning during early vascular development in the vertebrate embryo is regulated by paracrine signalling.
format	article
author	Alvaro Köhn-Luque Walter de Back Jörn Starruss Andrea Mattiotti Andreas Deutsch José María Pérez-Pomares Miguel A Herrero
author_facet	Alvaro Köhn-Luque Walter de Back Jörn Starruss Andrea Mattiotti Andreas Deutsch José María Pérez-Pomares Miguel A Herrero
author_sort	Alvaro Köhn-Luque
title	Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
title_short	Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
title_full	Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
title_fullStr	Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
title_full_unstemmed	Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
title_sort	early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/e2d3b5533aaa492e91ae6cdc8021063b
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Early embryonic vascular patterning by matrix-mediated paracrine signalling: a mathematical model study.

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