GGNBP2 is necessary for testis morphology and sperm development

Abstract Gametogenetin Binding Protein 2 (GGNBP2) was identified as a tumor suppressor and verified as such by several studies. GGNBP2 has also been reported to be essential for pregnancy maintenance via regulation of trophoblast stem cells. Gametogenetin (GGN) is a testicular germ cell-specific gen...

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Autores principales:	Anqi Chen, Jixi Li, Lesheng Song, Chaoneng Ji, Marion Böing, Jinzhong Chen, Beate Brand-Saberi
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/e2d42553b2484de9afe4c1ea8a7c7426
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spelling	oai:doaj.org-article:e2d42553b2484de9afe4c1ea8a7c74262021-12-02T12:31:59ZGGNBP2 is necessary for testis morphology and sperm development10.1038/s41598-017-03193-y2045-2322https://doaj.org/article/e2d42553b2484de9afe4c1ea8a7c74262017-06-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-03193-yhttps://doaj.org/toc/2045-2322Abstract Gametogenetin Binding Protein 2 (GGNBP2) was identified as a tumor suppressor and verified as such by several studies. GGNBP2 has also been reported to be essential for pregnancy maintenance via regulation of trophoblast stem cells. Gametogenetin (GGN) is a testicular germ cell-specific gene expressed in adult testes. As a potential GGN1-interacting protein, the role of GGNBP2 in spermatogenesis has not yet been clarified. We generated heterozygous GGNBP2 knockout mice and bred them by intercrossing. We found that among the offspring, homozygous GGNBP2 knockout (KO) mice were present in severely reduced numbers. The GGNBP2 KO pups developed normally, but the male siblings showed dramatically reduced fertility. In these male homozygous GGNBP2 KO mice, the only pathological finding was abnormal morphology of the testes and absence of spermatozoa. In addition, increased apoptosis was observed in the testes of GGNBP2 KO mice. SOX9 staining revealed that SOX9-positive Sertoli cells were absent in the seminiferous tubules. In homozygous mice, proliferating cell nuclear antigen (PCNA)-positive cells were localized in the lumen of the convoluted seminiferous tubules. These results suggest that GGNBP2 plays a key role in spermatogenesis by affecting the morphology and function of SOX9-positive Sertoli cells.Anqi ChenJixi LiLesheng SongChaoneng JiMarion BöingJinzhong ChenBeate Brand-SaberiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-8 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Anqi Chen Jixi Li Lesheng Song Chaoneng Ji Marion Böing Jinzhong Chen Beate Brand-Saberi GGNBP2 is necessary for testis morphology and sperm development
description	Abstract Gametogenetin Binding Protein 2 (GGNBP2) was identified as a tumor suppressor and verified as such by several studies. GGNBP2 has also been reported to be essential for pregnancy maintenance via regulation of trophoblast stem cells. Gametogenetin (GGN) is a testicular germ cell-specific gene expressed in adult testes. As a potential GGN1-interacting protein, the role of GGNBP2 in spermatogenesis has not yet been clarified. We generated heterozygous GGNBP2 knockout mice and bred them by intercrossing. We found that among the offspring, homozygous GGNBP2 knockout (KO) mice were present in severely reduced numbers. The GGNBP2 KO pups developed normally, but the male siblings showed dramatically reduced fertility. In these male homozygous GGNBP2 KO mice, the only pathological finding was abnormal morphology of the testes and absence of spermatozoa. In addition, increased apoptosis was observed in the testes of GGNBP2 KO mice. SOX9 staining revealed that SOX9-positive Sertoli cells were absent in the seminiferous tubules. In homozygous mice, proliferating cell nuclear antigen (PCNA)-positive cells were localized in the lumen of the convoluted seminiferous tubules. These results suggest that GGNBP2 plays a key role in spermatogenesis by affecting the morphology and function of SOX9-positive Sertoli cells.
format	article
author	Anqi Chen Jixi Li Lesheng Song Chaoneng Ji Marion Böing Jinzhong Chen Beate Brand-Saberi
author_facet	Anqi Chen Jixi Li Lesheng Song Chaoneng Ji Marion Böing Jinzhong Chen Beate Brand-Saberi
author_sort	Anqi Chen
title	GGNBP2 is necessary for testis morphology and sperm development
title_short	GGNBP2 is necessary for testis morphology and sperm development
title_full	GGNBP2 is necessary for testis morphology and sperm development
title_fullStr	GGNBP2 is necessary for testis morphology and sperm development
title_full_unstemmed	GGNBP2 is necessary for testis morphology and sperm development
title_sort	ggnbp2 is necessary for testis morphology and sperm development
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/e2d42553b2484de9afe4c1ea8a7c7426
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GGNBP2 is necessary for testis morphology and sperm development

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