Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies

Shih-Jer Huang,1 Tzu-Pin Wang,1 Sheng-I Lue,2 Li-Fang Wang11Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan; 2Department of Physiology, Kaohsiung Medical University, Kaohsiung, TaiwanAbstract: Cationic polymers are one of the major nonviral gene deliver...

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Autores principales:	Huang SJ, Wang TP, Lue SI, Wang LF
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2013
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/e2d4a02d5525482ca303e777e9baa9f8
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spelling	oai:doaj.org-article:e2d4a02d5525482ca303e777e9baa9f82021-12-02T05:00:26ZPentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies1176-91141178-2013https://doaj.org/article/e2d4a02d5525482ca303e777e9baa9f82013-05-01T00:00:00Zhttp://www.dovepress.com/pentablock-copolymers-of-pluronic-f127-and-modified-poly2-dimethyl-ami-a13168https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Shih-Jer Huang,1 Tzu-Pin Wang,1 Sheng-I Lue,2 Li-Fang Wang11Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan; 2Department of Physiology, Kaohsiung Medical University, Kaohsiung, TaiwanAbstract: Cationic polymers are one of the major nonviral gene delivery vectors investigated in the past decade. In this study, we synthesized several cationic copolymers using atom transfer radical polymerization (ATRP) for gene delivery vectors: pluronic F127-poly(dimethylaminoethyl methacrylate) (PF127-pDMAEMA), pluronic F127-poly (dimethylaminoethyl methacrylate-tert-butyl acrylate) (PF127-p(DMAEMA-tBA)), and pluronic F127-poly(dimethylaminoethyl methacrylate-acrylic acid) (PF127-p(DMAEMA-AA)). The copolymers showed high buffering capacity and efficiently complexed with plasmid deoxyribonucleic acid (pDNA) to form nanoparticles 80–180 nm in diameter and with positive zeta potentials. In the absence of 10% fetal bovine serum, PF127-p(DMAEMA-AA) showed the highest gene expression and the lowest cytotoxicity in 293T cells. After acrylic acid groups had been linked with a fluorescent dye, the confocal laser scanning microscopic image showed that PF127-p(DMAEMA-AA)/pDNA could efficiently enter the cells. Both clathrin-mediated and caveolae-mediated endocytosis mechanisms were involved. Our results showed that PF127-p(DMAEMA-AA) has great potential to be a gene delivery vector.Keywords: nonviral vector, pluronic F127, dimethylaminoethyl methacrylate, copolymer, atom transfer radical polymerizationHuang SJWang TPLue SIWang LFDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2013, Iss default, Pp 2011-2027 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Huang SJ Wang TP Lue SI Wang LF Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
description	Shih-Jer Huang,1 Tzu-Pin Wang,1 Sheng-I Lue,2 Li-Fang Wang11Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, Taiwan; 2Department of Physiology, Kaohsiung Medical University, Kaohsiung, TaiwanAbstract: Cationic polymers are one of the major nonviral gene delivery vectors investigated in the past decade. In this study, we synthesized several cationic copolymers using atom transfer radical polymerization (ATRP) for gene delivery vectors: pluronic F127-poly(dimethylaminoethyl methacrylate) (PF127-pDMAEMA), pluronic F127-poly (dimethylaminoethyl methacrylate-tert-butyl acrylate) (PF127-p(DMAEMA-tBA)), and pluronic F127-poly(dimethylaminoethyl methacrylate-acrylic acid) (PF127-p(DMAEMA-AA)). The copolymers showed high buffering capacity and efficiently complexed with plasmid deoxyribonucleic acid (pDNA) to form nanoparticles 80–180 nm in diameter and with positive zeta potentials. In the absence of 10% fetal bovine serum, PF127-p(DMAEMA-AA) showed the highest gene expression and the lowest cytotoxicity in 293T cells. After acrylic acid groups had been linked with a fluorescent dye, the confocal laser scanning microscopic image showed that PF127-p(DMAEMA-AA)/pDNA could efficiently enter the cells. Both clathrin-mediated and caveolae-mediated endocytosis mechanisms were involved. Our results showed that PF127-p(DMAEMA-AA) has great potential to be a gene delivery vector.Keywords: nonviral vector, pluronic F127, dimethylaminoethyl methacrylate, copolymer, atom transfer radical polymerization
format	article
author	Huang SJ Wang TP Lue SI Wang LF
author_facet	Huang SJ Wang TP Lue SI Wang LF
author_sort	Huang SJ
title	Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
title_short	Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
title_full	Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
title_fullStr	Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
title_full_unstemmed	Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
title_sort	pentablock copolymers of pluronic f127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies
publisher	Dove Medical Press
publishDate	2013
url	https://doaj.org/article/e2d4a02d5525482ca303e777e9baa9f8
work_keys_str_mv	AT huangsj pentablockcopolymersofpluronicf127andmodifiedpoly2dimethylaminoethylmethacrylateforinternalizationmechanismandgenetransfectionstudies AT wangtp pentablockcopolymersofpluronicf127andmodifiedpoly2dimethylaminoethylmethacrylateforinternalizationmechanismandgenetransfectionstudies AT luesi pentablockcopolymersofpluronicf127andmodifiedpoly2dimethylaminoethylmethacrylateforinternalizationmechanismandgenetransfectionstudies AT wanglf pentablockcopolymersofpluronicf127andmodifiedpoly2dimethylaminoethylmethacrylateforinternalizationmechanismandgenetransfectionstudies
_version_	1718400861837721600

Pentablock copolymers of pluronic F127 and modified poly(2-dimethyl amino)ethyl methacrylate for internalization mechanism and gene transfection studies

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